Interleukin-17A as a Biomarker for Bovine Tuberculosis

W. Ray Waters, Mayara F. Maggioli, Mitchell V. Palmer, Tyler C. Thacker, Jodi L. McGill, H. Martin Vordermeier, Linda Berney-Meyer, William R. Jacobs, Michelle H. Larsen

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

T helper 17 (Th17)-associated cytokines are integral to the immune responses to tuberculosis, initiating both protective and harmful inflammatory responses. The aim of the present study was to evaluate applied aspects of interleukin-17 (IL-17) biology in the context of Mycobacterium bovis infection of cattle. Using transcriptome sequencing (RNA-Seq), numerous Th17-associated cytokine genes (including IL-17A, IL-17F, IL-22, IL-19, and IL-27) were upregulated>9-fold in response to purified protein derivative stimulation of peripheral blood mononuclear cells from experimentally M. bovis-infected cattle. Protective vaccines elicited IL-17A, IL-17F, IL-22, and IL-27 responses. Reduced IL-17A responses by vaccine recipients, compared to nonvaccinated animals, at 2.5 weeks after M. bovis challenge correlated with reduced disease burdens. Additionally, IL-17A and interferon gamma (IFN-γ) responses were highly correlated and exhibited similar diagnostic capacities. The present findings support the use of Th17-associated cytokines as biomarkers of infection and protection in the immune responses to bovine tuberculosis.

Original languageEnglish (US)
Pages (from-to)168-180
Number of pages13
JournalClinical and Vaccine Immunology
Volume23
Issue number2
DOIs
StatePublished - Feb 1 2016

Fingerprint

Bovine Tuberculosis
Interleukin-17
Biomarkers
Mycobacterium bovis
Interleukin-27
Cytokines
Vaccines
RNA Sequence Analysis
Mycobacterium Infections
Transcriptome
Interferon-gamma
Blood Cells
Animals
Tuberculosis
Blood
Genes
RNA
Derivatives
Infection

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Immunology
  • Immunology and Allergy
  • Microbiology (medical)

Cite this

Waters, W. R., Maggioli, M. F., Palmer, M. V., Thacker, T. C., McGill, J. L., Vordermeier, H. M., ... Larsen, M. H. (2016). Interleukin-17A as a Biomarker for Bovine Tuberculosis. Clinical and Vaccine Immunology, 23(2), 168-180. https://doi.org/10.1128/CVI.00637-15

Interleukin-17A as a Biomarker for Bovine Tuberculosis. / Waters, W. Ray; Maggioli, Mayara F.; Palmer, Mitchell V.; Thacker, Tyler C.; McGill, Jodi L.; Vordermeier, H. Martin; Berney-Meyer, Linda; Jacobs, William R.; Larsen, Michelle H.

In: Clinical and Vaccine Immunology, Vol. 23, No. 2, 01.02.2016, p. 168-180.

Research output: Contribution to journalArticle

Waters, WR, Maggioli, MF, Palmer, MV, Thacker, TC, McGill, JL, Vordermeier, HM, Berney-Meyer, L, Jacobs, WR & Larsen, MH 2016, 'Interleukin-17A as a Biomarker for Bovine Tuberculosis', Clinical and Vaccine Immunology, vol. 23, no. 2, pp. 168-180. https://doi.org/10.1128/CVI.00637-15
Waters WR, Maggioli MF, Palmer MV, Thacker TC, McGill JL, Vordermeier HM et al. Interleukin-17A as a Biomarker for Bovine Tuberculosis. Clinical and Vaccine Immunology. 2016 Feb 1;23(2):168-180. https://doi.org/10.1128/CVI.00637-15
Waters, W. Ray ; Maggioli, Mayara F. ; Palmer, Mitchell V. ; Thacker, Tyler C. ; McGill, Jodi L. ; Vordermeier, H. Martin ; Berney-Meyer, Linda ; Jacobs, William R. ; Larsen, Michelle H. / Interleukin-17A as a Biomarker for Bovine Tuberculosis. In: Clinical and Vaccine Immunology. 2016 ; Vol. 23, No. 2. pp. 168-180.
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