Interleukin 10 responses are associated with sustained CD4 T-Cell counts in treated HIV infection

Maria C. Villacres, Naoko Kono, Wendy J. MacK, Marek J. Nowicki, Kathryn Anastos, Michael Augenbraun, Chenglong Liu, Alan Landay, Ruth M. Greenblatt, Stephen J. Gange, Alexandra M. Levine

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Abstract

Background.Inflammation persists in treated human immunodeficiency virus (HIV) infection and may contribute to an increased risk for non-AIDS-related pathologies. We investigated the correlation of cytokine responses with changes in CD4 T-cell levels and coinfection with hepatitis C virus (HCV) during highly active antiretroviral treatment (HAART).Methods.A total of 383 participants in the Women's Interagency HIV Study (212 with HIV monoinfection, 56 with HCV monoinfection, and 115 with HIV/HCV coinfection) were studied. HIV-infected women had <1000 HIV RNA copies/mL, 99.7 had >200 CD4 T cells/L; 98 were receiving HAART at baseline. Changes in CD4 T-cell count between baseline and 2-4 years later were calculated. Peripheral blood mononuclear cells (PBMCs) obtained at baseline were used to measure interleukin 1 (IL-1), interleukin 6 (IL-6), interleukin 10 (IL-10), interleukin 12 (IL-12), and tumor necrosis factor (TNF-) responses to Toll-like receptor (TLR) 3 and TLR4 stimulation.Results.Undetectable HIV RNA (<80 copies/mL) at baseline and secretion of IL-10 by PBMCs were positively associated with gains in CD4 T-cell counts at follow-up. Inflammatory cytokines (IL-1, IL-6, IL-12, and TNF-) were also produced in TLR-stimulated cultures, but only IL-10 was significantly associated with sustained increases in CD4 T-cell levels. This association was significant only in women with HIV monoinfection, indicating that HCV coinfection is an important factor limiting gains in CD4 T-cell counts, possibly by contributing to unbalanced persistent inflammation.Conclusions.Secreted IL-10 from PBMCs may balance the inflammatory environment of HIV, resulting in CD4 T-cell stability.

Original languageEnglish (US)
Pages (from-to)780-789
Number of pages10
JournalJournal of Infectious Diseases
Volume206
Issue number5
DOIs
StatePublished - Sep 1 2012

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Virus Diseases
CD4 Lymphocyte Count
Interleukin-10
HIV
T-Lymphocytes
Hepacivirus
Coinfection
Blood Cells
Interleukin-12
Interleukin-1
Interleukin-6
Toll-Like Receptor 3
Cytokines
Inflammation
Toll-Like Receptors
Tumor Necrosis Factor-alpha
RNA
Pathology
Therapeutics

ASJC Scopus subject areas

  • Infectious Diseases
  • Immunology and Allergy

Cite this

Villacres, M. C., Kono, N., MacK, W. J., Nowicki, M. J., Anastos, K., Augenbraun, M., ... Levine, A. M. (2012). Interleukin 10 responses are associated with sustained CD4 T-Cell counts in treated HIV infection. Journal of Infectious Diseases, 206(5), 780-789. https://doi.org/10.1093/infdis/jis380

Interleukin 10 responses are associated with sustained CD4 T-Cell counts in treated HIV infection. / Villacres, Maria C.; Kono, Naoko; MacK, Wendy J.; Nowicki, Marek J.; Anastos, Kathryn; Augenbraun, Michael; Liu, Chenglong; Landay, Alan; Greenblatt, Ruth M.; Gange, Stephen J.; Levine, Alexandra M.

In: Journal of Infectious Diseases, Vol. 206, No. 5, 01.09.2012, p. 780-789.

Research output: Contribution to journalArticle

Villacres, MC, Kono, N, MacK, WJ, Nowicki, MJ, Anastos, K, Augenbraun, M, Liu, C, Landay, A, Greenblatt, RM, Gange, SJ & Levine, AM 2012, 'Interleukin 10 responses are associated with sustained CD4 T-Cell counts in treated HIV infection', Journal of Infectious Diseases, vol. 206, no. 5, pp. 780-789. https://doi.org/10.1093/infdis/jis380
Villacres, Maria C. ; Kono, Naoko ; MacK, Wendy J. ; Nowicki, Marek J. ; Anastos, Kathryn ; Augenbraun, Michael ; Liu, Chenglong ; Landay, Alan ; Greenblatt, Ruth M. ; Gange, Stephen J. ; Levine, Alexandra M. / Interleukin 10 responses are associated with sustained CD4 T-Cell counts in treated HIV infection. In: Journal of Infectious Diseases. 2012 ; Vol. 206, No. 5. pp. 780-789.
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abstract = "Background.Inflammation persists in treated human immunodeficiency virus (HIV) infection and may contribute to an increased risk for non-AIDS-related pathologies. We investigated the correlation of cytokine responses with changes in CD4 T-cell levels and coinfection with hepatitis C virus (HCV) during highly active antiretroviral treatment (HAART).Methods.A total of 383 participants in the Women's Interagency HIV Study (212 with HIV monoinfection, 56 with HCV monoinfection, and 115 with HIV/HCV coinfection) were studied. HIV-infected women had <1000 HIV RNA copies/mL, 99.7 had >200 CD4 T cells/L; 98 were receiving HAART at baseline. Changes in CD4 T-cell count between baseline and 2-4 years later were calculated. Peripheral blood mononuclear cells (PBMCs) obtained at baseline were used to measure interleukin 1 (IL-1), interleukin 6 (IL-6), interleukin 10 (IL-10), interleukin 12 (IL-12), and tumor necrosis factor (TNF-) responses to Toll-like receptor (TLR) 3 and TLR4 stimulation.Results.Undetectable HIV RNA (<80 copies/mL) at baseline and secretion of IL-10 by PBMCs were positively associated with gains in CD4 T-cell counts at follow-up. Inflammatory cytokines (IL-1, IL-6, IL-12, and TNF-) were also produced in TLR-stimulated cultures, but only IL-10 was significantly associated with sustained increases in CD4 T-cell levels. This association was significant only in women with HIV monoinfection, indicating that HCV coinfection is an important factor limiting gains in CD4 T-cell counts, possibly by contributing to unbalanced persistent inflammation.Conclusions.Secreted IL-10 from PBMCs may balance the inflammatory environment of HIV, resulting in CD4 T-cell stability.",
author = "Villacres, {Maria C.} and Naoko Kono and MacK, {Wendy J.} and Nowicki, {Marek J.} and Kathryn Anastos and Michael Augenbraun and Chenglong Liu and Alan Landay and Greenblatt, {Ruth M.} and Gange, {Stephen J.} and Levine, {Alexandra M.}",
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AU - Villacres, Maria C.

AU - Kono, Naoko

AU - MacK, Wendy J.

AU - Nowicki, Marek J.

AU - Anastos, Kathryn

AU - Augenbraun, Michael

AU - Liu, Chenglong

AU - Landay, Alan

AU - Greenblatt, Ruth M.

AU - Gange, Stephen J.

AU - Levine, Alexandra M.

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N2 - Background.Inflammation persists in treated human immunodeficiency virus (HIV) infection and may contribute to an increased risk for non-AIDS-related pathologies. We investigated the correlation of cytokine responses with changes in CD4 T-cell levels and coinfection with hepatitis C virus (HCV) during highly active antiretroviral treatment (HAART).Methods.A total of 383 participants in the Women's Interagency HIV Study (212 with HIV monoinfection, 56 with HCV monoinfection, and 115 with HIV/HCV coinfection) were studied. HIV-infected women had <1000 HIV RNA copies/mL, 99.7 had >200 CD4 T cells/L; 98 were receiving HAART at baseline. Changes in CD4 T-cell count between baseline and 2-4 years later were calculated. Peripheral blood mononuclear cells (PBMCs) obtained at baseline were used to measure interleukin 1 (IL-1), interleukin 6 (IL-6), interleukin 10 (IL-10), interleukin 12 (IL-12), and tumor necrosis factor (TNF-) responses to Toll-like receptor (TLR) 3 and TLR4 stimulation.Results.Undetectable HIV RNA (<80 copies/mL) at baseline and secretion of IL-10 by PBMCs were positively associated with gains in CD4 T-cell counts at follow-up. Inflammatory cytokines (IL-1, IL-6, IL-12, and TNF-) were also produced in TLR-stimulated cultures, but only IL-10 was significantly associated with sustained increases in CD4 T-cell levels. This association was significant only in women with HIV monoinfection, indicating that HCV coinfection is an important factor limiting gains in CD4 T-cell counts, possibly by contributing to unbalanced persistent inflammation.Conclusions.Secreted IL-10 from PBMCs may balance the inflammatory environment of HIV, resulting in CD4 T-cell stability.

AB - Background.Inflammation persists in treated human immunodeficiency virus (HIV) infection and may contribute to an increased risk for non-AIDS-related pathologies. We investigated the correlation of cytokine responses with changes in CD4 T-cell levels and coinfection with hepatitis C virus (HCV) during highly active antiretroviral treatment (HAART).Methods.A total of 383 participants in the Women's Interagency HIV Study (212 with HIV monoinfection, 56 with HCV monoinfection, and 115 with HIV/HCV coinfection) were studied. HIV-infected women had <1000 HIV RNA copies/mL, 99.7 had >200 CD4 T cells/L; 98 were receiving HAART at baseline. Changes in CD4 T-cell count between baseline and 2-4 years later were calculated. Peripheral blood mononuclear cells (PBMCs) obtained at baseline were used to measure interleukin 1 (IL-1), interleukin 6 (IL-6), interleukin 10 (IL-10), interleukin 12 (IL-12), and tumor necrosis factor (TNF-) responses to Toll-like receptor (TLR) 3 and TLR4 stimulation.Results.Undetectable HIV RNA (<80 copies/mL) at baseline and secretion of IL-10 by PBMCs were positively associated with gains in CD4 T-cell counts at follow-up. Inflammatory cytokines (IL-1, IL-6, IL-12, and TNF-) were also produced in TLR-stimulated cultures, but only IL-10 was significantly associated with sustained increases in CD4 T-cell levels. This association was significant only in women with HIV monoinfection, indicating that HCV coinfection is an important factor limiting gains in CD4 T-cell counts, possibly by contributing to unbalanced persistent inflammation.Conclusions.Secreted IL-10 from PBMCs may balance the inflammatory environment of HIV, resulting in CD4 T-cell stability.

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