Interlaboratory reproducibility of female genital tract cytokine measurements by Luminex: Implications for microbicide safety studies

Mark E. Scott, Sarah S. Wilson, Lisa A. Cosentino, Barbra A. Richardson, Anna Barbara Moscicki, Sharon L. Hillier, Betsy C. Herold

Research output: Contribution to journalArticle

11 Scopus citations

Abstract

The interlaboratory reproducibility of cytokine measurements from cervicovaginal samples by Luminex has not been reported. Using cervicovaginal lavage specimens collected on three study days from 12 women participating in a Phase I microbicide study, we measured a panel of eight cytokines in three independent laboratories. Four (IFN-γ, IL-10, IL-17, and TNF) were below the limit of detection in the majority (85%) of samples in either two or all three laboratories, an observation that may guide analyte selection for future studies. Good interlaboratory agreement (intraclass correlation coefficient, r>0.7) in absolute levels was observed for IL-1β, IL-6, and IL-8, while poor agreement was seen for IFN-α2 (r=0.47). When considering within-subject change from baseline (pre-product, at study-day 0) to either post-product visit (study-days 7 and 14), IL-1β and IL-6 exhibited good interlaboratory agreement (r>0.7), while IFN-α2 and IL-8 did not. Future studies addressing the clinical utility of specific biomarkers of inflammation for microbicide trials should consider reproducibility in the context of defining biologically meaningful thresholds of change for candidate biomarkers, ensuring that such change can be reliably distinguished from background variability.

Original languageEnglish (US)
Pages (from-to)430-434
Number of pages5
JournalCytokine
Volume56
Issue number2
DOIs
StatePublished - Nov 1 2011

Keywords

  • Cervicovaginal secretions
  • Cytokine measurement
  • Microbicide studies
  • Multiplex methods
  • Reproducibility

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Biochemistry
  • Hematology
  • Molecular Biology

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