Interferon-beta upregulates interleukin-1 receptor antagonist in human microglia

J. S H Liu, T. D. Amaral, C. F. Brosnan, S. C. Lee

Research output: Contribution to journalArticle

Abstract

Interleukin-1 is a cytokine implicated in the initiation of proinflammatory activities in a number of central nervous system diseases. The activities of IL-1 are regulated, in part, by a specific receptor antagonist, IL-1ra. In this study we assessed the effect of cytokines on IL-1β and IL-1ra mRNA and protein expression by human fetal glia. In microglia, both LPS and IL-1α were potent inducers of IL-1β, but only LPS co-induced IL-Ira. In contrast, IL-4 and IFNβ did not induce IL-1β production, but were potent inducers of IL-1ra and markedly down-regulated both LPS and IL-1α induced IL-1β production. In addition, when given together with LPS, IL-4 and IFNβ enhanced the production of IL-1ra. IL-10, TGFβ and IL-6 did not induce IL-1β, but showed variable induction of IL-1ra. IL-10 and TGFβ inhibited IL-1β expression induced by LPS and IL-1α, respectively. IL-1α/β did not affect the production of IL-1ra by any of the cytokines tested. Interestingly, IFNγ markedly inhibited both LPS and IL-1α induced IL-1β as well as LPS and IFNβ induced IL-1ra. This effect was not due to a generalized inhibition of transcription since LPS induction of TNFα was unaffected by IFNγ. Human fetal astrocytes could not be induced to express either IL-1α or IL-1ra. These results suggest that the production of IL-1ra by IFNβ and IL-4 contribute to the down-regulatory effect of these cytokines on inflammation in the CNS, but that IFNγ may have either pro-or anti-inflammatory activity depending upon the nature of the other cytokines present in the inflammatory microenvironment.

Original languageEnglish (US)
JournalFASEB Journal
Volume12
Issue number5
StatePublished - Mar 20 1998

Fingerprint

interferon-beta
Interleukin-1 Receptors
Interferon-beta
interleukin-1
neuroglia
Microglia
Interleukin-1
antagonists
Interleukin 1 Receptor Antagonist Protein
Up-Regulation
receptors
cytokines
Cytokines
interleukin-4
Interleukin-4
interleukin-10
Interleukin-10
central nervous system diseases
astrocytes
Central Nervous System Diseases

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

Cite this

Liu, J. S. H., Amaral, T. D., Brosnan, C. F., & Lee, S. C. (1998). Interferon-beta upregulates interleukin-1 receptor antagonist in human microglia. FASEB Journal, 12(5).

Interferon-beta upregulates interleukin-1 receptor antagonist in human microglia. / Liu, J. S H; Amaral, T. D.; Brosnan, C. F.; Lee, S. C.

In: FASEB Journal, Vol. 12, No. 5, 20.03.1998.

Research output: Contribution to journalArticle

Liu, JSH, Amaral, TD, Brosnan, CF & Lee, SC 1998, 'Interferon-beta upregulates interleukin-1 receptor antagonist in human microglia', FASEB Journal, vol. 12, no. 5.
Liu JSH, Amaral TD, Brosnan CF, Lee SC. Interferon-beta upregulates interleukin-1 receptor antagonist in human microglia. FASEB Journal. 1998 Mar 20;12(5).
Liu, J. S H ; Amaral, T. D. ; Brosnan, C. F. ; Lee, S. C. / Interferon-beta upregulates interleukin-1 receptor antagonist in human microglia. In: FASEB Journal. 1998 ; Vol. 12, No. 5.
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