Interferon activation of the transcription factor Stat91 involves dimerization through SH2-phosphotyrosyl peptide interactions

Ke Shuai, Curt M. Horvath, Linda H Tsai Huang, Sajjad A. Qureshi, David Cowburn, James E. Darnell

Research output: Contribution to journalArticle

630 Citations (Scopus)

Abstract

Stat91 (a 91 kd protein that acts as a signal transducer and activator of transcription) is inactive in the cytoplasm of untreated cells but is activated by phosphorylation on tyrosine in response to a number of polypeptide ligands, including interferon α (IFN-α) and IFN-γ. We report here that the inactive Stat91 in the cytoplasm of untreated cells is a monomer and that upon IFN-γ-induced phosphorylation it forms a stable homodimer. Only the dimer is capable of binding to a specific DNA sequence directing transcription. Through dissociation and reassociation assays, we show that dimerization of Stat91 is mediated through SH2-phosphotyrosyl peptide interactions. Dimerization involving SH2 recognition of specific phosphotyrosyl peptides may well provide a prototype for interactions among family members of STAT proteins to form different transcription complexes.

Original languageEnglish (US)
Pages (from-to)821-828
Number of pages8
JournalCell
Volume76
Issue number5
DOIs
StatePublished - Mar 11 1994
Externally publishedYes

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Dimerization
Transcription
Interferons
Phosphorylation
Transcription Factors
Chemical activation
Peptides
Cytoplasm
DNA sequences
Transducers
Dimers
Tyrosine
Assays
Proteins
Monomers
Ligands

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology

Cite this

Interferon activation of the transcription factor Stat91 involves dimerization through SH2-phosphotyrosyl peptide interactions. / Shuai, Ke; Horvath, Curt M.; Huang, Linda H Tsai; Qureshi, Sajjad A.; Cowburn, David; Darnell, James E.

In: Cell, Vol. 76, No. 5, 11.03.1994, p. 821-828.

Research output: Contribution to journalArticle

Shuai, Ke ; Horvath, Curt M. ; Huang, Linda H Tsai ; Qureshi, Sajjad A. ; Cowburn, David ; Darnell, James E. / Interferon activation of the transcription factor Stat91 involves dimerization through SH2-phosphotyrosyl peptide interactions. In: Cell. 1994 ; Vol. 76, No. 5. pp. 821-828.
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