Intercellular adhesion molecule-1 (ICAM-1) is upregulated in aggressive papillary thyroid carcinoma

D. Buitrago, X. M. Keutgen, M. Crowley, F. Filicori, H. Aldailami, R. Hoda, Yi Fang Liu, R. S. Hoda, T. Scognamiglio, M. Jin, T. J. Fahey, R. Zarnegar

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Background. Intercellular adhesion molecule-1 (ICAM-1) is implicated in carcinogenesis. In this study we examined the expression of ICAM-1 in papillary thyroid cancer (PTC). We hypothesized that ICAM-1 correlates with indicators of tumor aggressiveness in PTC. Methods. Thirty-five primary and metastatic PTCs, five follicular adenomas, five Hashimoto thyroiditis, five nodular hyperplasia, and eight normal thyroid tissue samples were analyzed for ICAM-1 gene expression using quantitative reverse-transcription polymerase chain reaction (RT-PCR). ICAM-1 gene expression was analyzed at protein level by immunohistochemistry (IHC) using a semiquantitative score. Gene expression and intensity levels were correlated with markers of tumor aggressiveness including BRAF V600E mutation, tumor size, extrathyroidal extension (ETE), angiolymphatic invasion, and lymph node metastasis. Results. ICAM-1 gene expression was higher in PTC (p = 0.01) and lymph node metastases (p = 0.03) when compared with benign tumors and Hashimoto's. Furthermore, PTCs exhibiting BRAF V600E mutation (p = 0.01), ETE (p\0.01), and lymph node metastasis (p = 0.02) were associated with higher ICAM-1 levels. Gene expression correlated with protein levels on IHC. Additionally, poorly differentiated thyroid carcinoma had a higher ICAM-1 intensity score compared with well-differentiated carcinoma (p = 0.03). Conclusions. ICAM-1 expression is upregulated in papillary thyroid carcinoma. Furthermore, ICAM-1 upregulation correlated with aggressive tumor features such as BRAF V600E mutation, ETE, and lymph node metastasis, suggesting that ICAM-1 plays a role in thyroid cancer progression.

Original languageEnglish (US)
Pages (from-to)973-980
Number of pages8
JournalAnnals of Surgical Oncology
Volume19
Issue number3
DOIs
StatePublished - Mar 1 2012
Externally publishedYes

Fingerprint

Intercellular Adhesion Molecule-1
Gene Expression
Lymph Nodes
Neoplasm Metastasis
Factor IX
Thyroid Neoplasms
Mutation
Neoplasms
Papillary Thyroid cancer
Immunohistochemistry
Hashimoto Disease
Tumor Biomarkers
Adenoma
Reverse Transcription
Hyperplasia
Thyroid Gland
Carcinogenesis
Proteins
Up-Regulation
Carcinoma

ASJC Scopus subject areas

  • Surgery
  • Oncology

Cite this

Intercellular adhesion molecule-1 (ICAM-1) is upregulated in aggressive papillary thyroid carcinoma. / Buitrago, D.; Keutgen, X. M.; Crowley, M.; Filicori, F.; Aldailami, H.; Hoda, R.; Liu, Yi Fang; Hoda, R. S.; Scognamiglio, T.; Jin, M.; Fahey, T. J.; Zarnegar, R.

In: Annals of Surgical Oncology, Vol. 19, No. 3, 01.03.2012, p. 973-980.

Research output: Contribution to journalArticle

Buitrago, D, Keutgen, XM, Crowley, M, Filicori, F, Aldailami, H, Hoda, R, Liu, YF, Hoda, RS, Scognamiglio, T, Jin, M, Fahey, TJ & Zarnegar, R 2012, 'Intercellular adhesion molecule-1 (ICAM-1) is upregulated in aggressive papillary thyroid carcinoma', Annals of Surgical Oncology, vol. 19, no. 3, pp. 973-980. https://doi.org/10.1245/s10434-011-2029-0
Buitrago, D. ; Keutgen, X. M. ; Crowley, M. ; Filicori, F. ; Aldailami, H. ; Hoda, R. ; Liu, Yi Fang ; Hoda, R. S. ; Scognamiglio, T. ; Jin, M. ; Fahey, T. J. ; Zarnegar, R. / Intercellular adhesion molecule-1 (ICAM-1) is upregulated in aggressive papillary thyroid carcinoma. In: Annals of Surgical Oncology. 2012 ; Vol. 19, No. 3. pp. 973-980.
@article{ed4c8418dcaa4740aace72f4880d2fc4,
title = "Intercellular adhesion molecule-1 (ICAM-1) is upregulated in aggressive papillary thyroid carcinoma",
abstract = "Background. Intercellular adhesion molecule-1 (ICAM-1) is implicated in carcinogenesis. In this study we examined the expression of ICAM-1 in papillary thyroid cancer (PTC). We hypothesized that ICAM-1 correlates with indicators of tumor aggressiveness in PTC. Methods. Thirty-five primary and metastatic PTCs, five follicular adenomas, five Hashimoto thyroiditis, five nodular hyperplasia, and eight normal thyroid tissue samples were analyzed for ICAM-1 gene expression using quantitative reverse-transcription polymerase chain reaction (RT-PCR). ICAM-1 gene expression was analyzed at protein level by immunohistochemistry (IHC) using a semiquantitative score. Gene expression and intensity levels were correlated with markers of tumor aggressiveness including BRAF V600E mutation, tumor size, extrathyroidal extension (ETE), angiolymphatic invasion, and lymph node metastasis. Results. ICAM-1 gene expression was higher in PTC (p = 0.01) and lymph node metastases (p = 0.03) when compared with benign tumors and Hashimoto's. Furthermore, PTCs exhibiting BRAF V600E mutation (p = 0.01), ETE (p\0.01), and lymph node metastasis (p = 0.02) were associated with higher ICAM-1 levels. Gene expression correlated with protein levels on IHC. Additionally, poorly differentiated thyroid carcinoma had a higher ICAM-1 intensity score compared with well-differentiated carcinoma (p = 0.03). Conclusions. ICAM-1 expression is upregulated in papillary thyroid carcinoma. Furthermore, ICAM-1 upregulation correlated with aggressive tumor features such as BRAF V600E mutation, ETE, and lymph node metastasis, suggesting that ICAM-1 plays a role in thyroid cancer progression.",
author = "D. Buitrago and Keutgen, {X. M.} and M. Crowley and F. Filicori and H. Aldailami and R. Hoda and Liu, {Yi Fang} and Hoda, {R. S.} and T. Scognamiglio and M. Jin and Fahey, {T. J.} and R. Zarnegar",
year = "2012",
month = "3",
day = "1",
doi = "10.1245/s10434-011-2029-0",
language = "English (US)",
volume = "19",
pages = "973--980",
journal = "Annals of Surgical Oncology",
issn = "1068-9265",
publisher = "Springer New York",
number = "3",

}

TY - JOUR

T1 - Intercellular adhesion molecule-1 (ICAM-1) is upregulated in aggressive papillary thyroid carcinoma

AU - Buitrago, D.

AU - Keutgen, X. M.

AU - Crowley, M.

AU - Filicori, F.

AU - Aldailami, H.

AU - Hoda, R.

AU - Liu, Yi Fang

AU - Hoda, R. S.

AU - Scognamiglio, T.

AU - Jin, M.

AU - Fahey, T. J.

AU - Zarnegar, R.

PY - 2012/3/1

Y1 - 2012/3/1

N2 - Background. Intercellular adhesion molecule-1 (ICAM-1) is implicated in carcinogenesis. In this study we examined the expression of ICAM-1 in papillary thyroid cancer (PTC). We hypothesized that ICAM-1 correlates with indicators of tumor aggressiveness in PTC. Methods. Thirty-five primary and metastatic PTCs, five follicular adenomas, five Hashimoto thyroiditis, five nodular hyperplasia, and eight normal thyroid tissue samples were analyzed for ICAM-1 gene expression using quantitative reverse-transcription polymerase chain reaction (RT-PCR). ICAM-1 gene expression was analyzed at protein level by immunohistochemistry (IHC) using a semiquantitative score. Gene expression and intensity levels were correlated with markers of tumor aggressiveness including BRAF V600E mutation, tumor size, extrathyroidal extension (ETE), angiolymphatic invasion, and lymph node metastasis. Results. ICAM-1 gene expression was higher in PTC (p = 0.01) and lymph node metastases (p = 0.03) when compared with benign tumors and Hashimoto's. Furthermore, PTCs exhibiting BRAF V600E mutation (p = 0.01), ETE (p\0.01), and lymph node metastasis (p = 0.02) were associated with higher ICAM-1 levels. Gene expression correlated with protein levels on IHC. Additionally, poorly differentiated thyroid carcinoma had a higher ICAM-1 intensity score compared with well-differentiated carcinoma (p = 0.03). Conclusions. ICAM-1 expression is upregulated in papillary thyroid carcinoma. Furthermore, ICAM-1 upregulation correlated with aggressive tumor features such as BRAF V600E mutation, ETE, and lymph node metastasis, suggesting that ICAM-1 plays a role in thyroid cancer progression.

AB - Background. Intercellular adhesion molecule-1 (ICAM-1) is implicated in carcinogenesis. In this study we examined the expression of ICAM-1 in papillary thyroid cancer (PTC). We hypothesized that ICAM-1 correlates with indicators of tumor aggressiveness in PTC. Methods. Thirty-five primary and metastatic PTCs, five follicular adenomas, five Hashimoto thyroiditis, five nodular hyperplasia, and eight normal thyroid tissue samples were analyzed for ICAM-1 gene expression using quantitative reverse-transcription polymerase chain reaction (RT-PCR). ICAM-1 gene expression was analyzed at protein level by immunohistochemistry (IHC) using a semiquantitative score. Gene expression and intensity levels were correlated with markers of tumor aggressiveness including BRAF V600E mutation, tumor size, extrathyroidal extension (ETE), angiolymphatic invasion, and lymph node metastasis. Results. ICAM-1 gene expression was higher in PTC (p = 0.01) and lymph node metastases (p = 0.03) when compared with benign tumors and Hashimoto's. Furthermore, PTCs exhibiting BRAF V600E mutation (p = 0.01), ETE (p\0.01), and lymph node metastasis (p = 0.02) were associated with higher ICAM-1 levels. Gene expression correlated with protein levels on IHC. Additionally, poorly differentiated thyroid carcinoma had a higher ICAM-1 intensity score compared with well-differentiated carcinoma (p = 0.03). Conclusions. ICAM-1 expression is upregulated in papillary thyroid carcinoma. Furthermore, ICAM-1 upregulation correlated with aggressive tumor features such as BRAF V600E mutation, ETE, and lymph node metastasis, suggesting that ICAM-1 plays a role in thyroid cancer progression.

UR - http://www.scopus.com/inward/record.url?scp=84862247454&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84862247454&partnerID=8YFLogxK

U2 - 10.1245/s10434-011-2029-0

DO - 10.1245/s10434-011-2029-0

M3 - Article

C2 - 21879273

AN - SCOPUS:84862247454

VL - 19

SP - 973

EP - 980

JO - Annals of Surgical Oncology

JF - Annals of Surgical Oncology

SN - 1068-9265

IS - 3

ER -