Intercalary heterochromatin in Drosophila melanogaster polytene chromosomes and the problem of genetic silencing

Igor F. Zhimulev; Elena S. Belyaeva; Igor V. Makunin; Vincenzo Pirrotta; Valery F. Semeshin; Artyom A. Alekseyenko; Stepan N. Belyakin; Elena I. Volkova; Dmitry E. Koryakov; Evgeniya N. Andreyeva; Olga V. Demakova; Irina V. Kotlikova; Tatyana D. Kolesnikova; Lidiya V. Boldyreva; Roman A. Nanayev

doi:10.1023/A:1022912716376

Intercalary heterochromatin in Drosophila melanogaster polytene chromosomes and the problem of genetic silencing

Igor F. Zhimulev, Elena S. Belyaeva, Igor V. Makunin, Vincenzo Pirrotta, Valery F. Semeshin, Artyom A. Alekseyenko, Stepan N. Belyakin, Elena I. Volkova, Dmitry E. Koryakov, Evgeniya N. Andreyeva, Olga V. Demakova, Irina V. Kotlikova, Tatyana D. Kolesnikova, Lidiya V. Boldyreva, Roman A. Nanayev

Research output: Contribution to journal › Review article › peer-review

16 Scopus citations

Abstract

The morphological characteristics of intercalary heterochromatin (IH) are compared with those of other types of silenced chromatin in the Drosophila melanogaster genome: pericentric heterochromatin (PH) and regions subject to position effect variegation (PEV). We conclude that IH regions in polytene chromosomes are binding sites of silencing complexes such as PcG complexes and of SuUR protein. Binding of these proteins results in the appearance of condensed chromatin and late replication of DNA, which in turn may result in DNA underreplication. IH and PH as well as regions subject to PEV have in common the condensed chromatin appearance, the localization of specific proteins, late replication, underreplication in polytene chromosomes, and ectopic pairing.

Original language	English (US)
Pages (from-to)	259-270
Number of pages	12
Journal	Genetica
Volume	117
Issue number	2-3
DOIs	https://doi.org/10.1023/A:1022912716376
State	Published - Mar 2003
Externally published	Yes

Keywords

Epigenetic silencing
Intercalary heterochromatin
Pericentric heterochromatin
Position effect variegation
SuUR

ASJC Scopus subject areas

Animal Science and Zoology
Genetics
Plant Science
Insect Science

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Zhimulev, I. F., Belyaeva, E. S., Makunin, I. V., Pirrotta, V., Semeshin, V. F., Alekseyenko, A. A., Belyakin, S. N., Volkova, E. I., Koryakov, D. E., Andreyeva, E. N., Demakova, O. V., Kotlikova, I. V., Kolesnikova, T. D., Boldyreva, L. V., & Nanayev, R. A. (2003). Intercalary heterochromatin in Drosophila melanogaster polytene chromosomes and the problem of genetic silencing. Genetica, 117(2-3), 259-270. https://doi.org/10.1023/A:1022912716376

Zhimulev, IF, Belyaeva, ES, Makunin, IV, Pirrotta, V, Semeshin, VF, Alekseyenko, AA, Belyakin, SN, Volkova, EI, Koryakov, DE, Andreyeva, EN, Demakova, OV, Kotlikova, IV, Kolesnikova, TD, Boldyreva, LV & Nanayev, RA 2003, 'Intercalary heterochromatin in Drosophila melanogaster polytene chromosomes and the problem of genetic silencing', Genetica, vol. 117, no. 2-3, pp. 259-270. https://doi.org/10.1023/A:1022912716376

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title = "Intercalary heterochromatin in Drosophila melanogaster polytene chromosomes and the problem of genetic silencing",

abstract = "The morphological characteristics of intercalary heterochromatin (IH) are compared with those of other types of silenced chromatin in the Drosophila melanogaster genome: pericentric heterochromatin (PH) and regions subject to position effect variegation (PEV). We conclude that IH regions in polytene chromosomes are binding sites of silencing complexes such as PcG complexes and of SuUR protein. Binding of these proteins results in the appearance of condensed chromatin and late replication of DNA, which in turn may result in DNA underreplication. IH and PH as well as regions subject to PEV have in common the condensed chromatin appearance, the localization of specific proteins, late replication, underreplication in polytene chromosomes, and ectopic pairing.",

keywords = "Epigenetic silencing, Intercalary heterochromatin, Pericentric heterochromatin, Position effect variegation, SuUR",

author = "Zhimulev, {Igor F.} and Belyaeva, {Elena S.} and Makunin, {Igor V.} and Vincenzo Pirrotta and Semeshin, {Valery F.} and Alekseyenko, {Artyom A.} and Belyakin, {Stepan N.} and Volkova, {Elena I.} and Koryakov, {Dmitry E.} and Andreyeva, {Evgeniya N.} and Demakova, {Olga V.} and Kotlikova, {Irina V.} and Kolesnikova, {Tatyana D.} and Boldyreva, {Lidiya V.} and Nanayev, {Roman A.}",

note = "Funding Information: The work was supported by grants from the program “Frontiers in Genetics of the Russian Federation” 99-2-020, the RFBR program N99-04-49270 and 00-15-97984, INTAS grant N99-1088, The Institutional Partnership program of the Swiss National Science Foundation (SCOPES) and from the University of Geneva.",

year = "2003",

month = mar,

doi = "10.1023/A:1022912716376",

language = "English (US)",

volume = "117",

pages = "259--270",

journal = "Genetica",

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T1 - Intercalary heterochromatin in Drosophila melanogaster polytene chromosomes and the problem of genetic silencing

AU - Zhimulev, Igor F.

AU - Belyaeva, Elena S.

AU - Makunin, Igor V.

AU - Pirrotta, Vincenzo

AU - Semeshin, Valery F.

AU - Alekseyenko, Artyom A.

AU - Belyakin, Stepan N.

AU - Volkova, Elena I.

AU - Koryakov, Dmitry E.

AU - Andreyeva, Evgeniya N.

AU - Demakova, Olga V.

AU - Kotlikova, Irina V.

AU - Kolesnikova, Tatyana D.

AU - Boldyreva, Lidiya V.

AU - Nanayev, Roman A.

N1 - Funding Information: The work was supported by grants from the program “Frontiers in Genetics of the Russian Federation” 99-2-020, the RFBR program N99-04-49270 and 00-15-97984, INTAS grant N99-1088, The Institutional Partnership program of the Swiss National Science Foundation (SCOPES) and from the University of Geneva.

PY - 2003/3

Y1 - 2003/3

N2 - The morphological characteristics of intercalary heterochromatin (IH) are compared with those of other types of silenced chromatin in the Drosophila melanogaster genome: pericentric heterochromatin (PH) and regions subject to position effect variegation (PEV). We conclude that IH regions in polytene chromosomes are binding sites of silencing complexes such as PcG complexes and of SuUR protein. Binding of these proteins results in the appearance of condensed chromatin and late replication of DNA, which in turn may result in DNA underreplication. IH and PH as well as regions subject to PEV have in common the condensed chromatin appearance, the localization of specific proteins, late replication, underreplication in polytene chromosomes, and ectopic pairing.

AB - The morphological characteristics of intercalary heterochromatin (IH) are compared with those of other types of silenced chromatin in the Drosophila melanogaster genome: pericentric heterochromatin (PH) and regions subject to position effect variegation (PEV). We conclude that IH regions in polytene chromosomes are binding sites of silencing complexes such as PcG complexes and of SuUR protein. Binding of these proteins results in the appearance of condensed chromatin and late replication of DNA, which in turn may result in DNA underreplication. IH and PH as well as regions subject to PEV have in common the condensed chromatin appearance, the localization of specific proteins, late replication, underreplication in polytene chromosomes, and ectopic pairing.

KW - Epigenetic silencing

KW - Intercalary heterochromatin

KW - Pericentric heterochromatin

KW - Position effect variegation

KW - SuUR

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U2 - 10.1023/A:1022912716376

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SN - 0016-6707

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JO - Genetica

JF - Genetica

IS - 2-3

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Intercalary heterochromatin in Drosophila melanogaster polytene chromosomes and the problem of genetic silencing

Abstract

Keywords

ASJC Scopus subject areas

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