Interactions of methylmercury with rat primary astrocyte cultures: inhibition of rubidium and glutamate uptake and induction of swelling

Michael Aschner, N. B. Eberle, K. Miller, H. K. Kimelberg

Research output: Contribution to journalArticle

57 Citations (Scopus)

Abstract

The ability of astrocytes to sequester MeHG may indicate an astrocyte-mediated role in MeHg's neurotoxicity. Hence, studies were undertaken to assess the effects of MeHg on metabolic functions in cultured astrocytes. MeHg (10-5 M) significantly inhibited the initial rate (5 min) of uptake of 86RbCl, used as a tracer for K+. 86RbCl uptake was also sensitive to the omission of medium Na+. MeHg (10-5 M) also markedly inhibited the initial rate of uptake (1 min) of the Na+-dependent uptake of [3H]l-glutamate. A second neurotoxin, MnCl2 (0-5 × 10-4 M), did not alter [3H]glutamate or 86RbCl uptake. MeHg, but not MnCl2, also stimulated the release of intracellular 86Rb+ in a dose-dependent fashion. This effect could be prevented by the administration of MeHg as the glutathione conjugate. These observations support the hypothesis that the astrocyte plasma membrane is an important target for MeHg's toxic effect and specifically that small concentrations of this organometal inhibit the ability of astrocytes to maintain a transmembrane K+ gradient. This would be expected to compromise the ability of astrocytes to control extracellular K+ either by spatial buffering or active uptake, resulting in cellular swelling. We therefore studied volume changes in astrocytes using uptake of [14C]3-O-methyl-d-glucose, in attached cells in response to exposure to MeHg. Exposure to MeHg (0-5 × 10-4 M) caused a marked increase in the cell volume that was proportional to concentrations of MeHg.

Original languageEnglish (US)
Pages (from-to)245-250
Number of pages6
JournalBrain Research
Volume530
Issue number2
DOIs
StatePublished - Oct 22 1990
Externally publishedYes

Fingerprint

Rubidium
Astrocytes
Glutamic Acid
3-O-Methylglucose
Poisons
Neurotoxins
Cell Size
Glutathione
Cell Membrane

Keywords

  • Astrocyte
  • Glutamate
  • Methylmercury
  • Na, K-ATPase
  • Rubidium

ASJC Scopus subject areas

  • Developmental Biology
  • Molecular Biology
  • Clinical Neurology
  • Neuroscience(all)

Cite this

Interactions of methylmercury with rat primary astrocyte cultures : inhibition of rubidium and glutamate uptake and induction of swelling. / Aschner, Michael; Eberle, N. B.; Miller, K.; Kimelberg, H. K.

In: Brain Research, Vol. 530, No. 2, 22.10.1990, p. 245-250.

Research output: Contribution to journalArticle

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