Interactions between sickle hemoglobin fibers

Christopher W. Jones, Jiang Cheng Wang, Frank A. Ferrone, Robin W. Briehl, Matthew S. Turner

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

We report on observations of "zippering" that occurs when two sickle hemoglobin fibers come together side by side. A transient Y-shaped object is formed which "zips" closed. We have been able to show how the strength of the interactions that drive this may be estimated by studying the frustrated structures sometimes formed between several fibers. Our measurements, when combined with mechanical constants determined by an analysis of bending fluctuations, allow us to make the first estimate of the magnitude of these interactions, of the order of 7kBT μm-1. Hemoglobin volume fractions of tens of % lead to significant depletion forces. We estimate the magnitude of both the depletion and Van der Waals forces between pairs of single fibers. We study how these are effected by the helical nature of the fibers and renormalised by bending fluctuations, calculations that could have wider applications beyond sickle hemoglobin fibers. Our theoretical analysis of single fibers is in encouraging, although not fully quantitative, agreement with our measurements. We conclude that the physics and rheology of the hemoglobin gel, as well as the pathology of sickle cell anemia itself, may be influenced by depletion interactions.

Original languageEnglish (US)
Pages (from-to)221-236
Number of pages16
JournalFaraday Discussions
Volume123
StatePublished - 2003

Fingerprint

Sickle Hemoglobin
hemoglobin
fibers
Fibers
depletion
interactions
Hemoglobins
anemias
Van der Waals forces
pathology
Pathology
estimates
Rheology
rheology
Volume fraction
Physics
Gels
gels
physics
cells

ASJC Scopus subject areas

  • Physical and Theoretical Chemistry

Cite this

Jones, C. W., Wang, J. C., Ferrone, F. A., Briehl, R. W., & Turner, M. S. (2003). Interactions between sickle hemoglobin fibers. Faraday Discussions, 123, 221-236.

Interactions between sickle hemoglobin fibers. / Jones, Christopher W.; Wang, Jiang Cheng; Ferrone, Frank A.; Briehl, Robin W.; Turner, Matthew S.

In: Faraday Discussions, Vol. 123, 2003, p. 221-236.

Research output: Contribution to journalArticle

Jones, CW, Wang, JC, Ferrone, FA, Briehl, RW & Turner, MS 2003, 'Interactions between sickle hemoglobin fibers', Faraday Discussions, vol. 123, pp. 221-236.
Jones CW, Wang JC, Ferrone FA, Briehl RW, Turner MS. Interactions between sickle hemoglobin fibers. Faraday Discussions. 2003;123:221-236.
Jones, Christopher W. ; Wang, Jiang Cheng ; Ferrone, Frank A. ; Briehl, Robin W. ; Turner, Matthew S. / Interactions between sickle hemoglobin fibers. In: Faraday Discussions. 2003 ; Vol. 123. pp. 221-236.
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