Interactions between proteins bound to the duck β^A-globin gene promoter and enhancer detected by the DNaseI footprinting

The duck β^A-globin (β^AGLB) enhancer was closely linked to the duck β^A-GLB promoter, and the construct was used to study binding of nuclear proteins to specific sites of these regulatory elements. DNaseI-footprint analysis showed that the presence of the enhancer induced binding of proteins to additional sites on the promoter. The results are consistent with the looping-out model, based on specific interactions of enhancer-bound and promoter bound-proteins.