Interaction of the microtubule cytoskeleton with endocytic vesicles and cytoplasmic dynein in cultured rat hepatocytes

H. Oda, R. J. Stockert, C. Collins, H. Wang, P. M. Novikoff, P. Satir, Allan W. Wolkoff

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Abstract

In a recent study (Goltz, J. S., Wolkoff, A. W., Novikoff, P. M., Stockert, R. J., and Satir, P. (1992) Proc. Natl. Acad. Sci. U. S. A. 89, 7026-7030), we found that ligand- and receptor-containing endocytic vesicles bind to endogenous microtubules in vitro after 60 min of receptor-mediated endocytosis of asialo-orosomucoid. In the presence of ATP, ligand-containing endocytic vesicles are released from microtubules, while those containing receptor are not. We hypothesized that cytoplasmic dynein may associate with ligand-containing, but not receptor-containing, domains of endocytic vesicles and might be involved in the movement of ligand-containing vesicles along microtubules during sorting of ligand from receptor. Direct evidence in support of this hypothesis has been obtained in the present study. Binding of ligand-containing vesicles to microtubules correlates highly (p < 0.001) with binding of dynein, but not kinesin, under a variety of conditions. Binding of receptor-containing vesicles to microtubules is independent of both cytoplasmic dynein and kinesin binding. Tight association of cytoplasmic dynein with a population of ligand-containing vesicles is seen directly by immunoprecipitation. These results support the view that in receptor-mediated endocytosis, ligand-containing vesicles become bound to microtubules by cytoplasmic dynein. While receptor domains of endosomes remain attached to microtubules in an ATP-independent manner, ligand-containing domains might be moved away toward pericentrosomal lysosomes by this motor molecule.

Original languageEnglish (US)
Pages (from-to)15242-15249
Number of pages8
JournalJournal of Biological Chemistry
Volume270
Issue number25
DOIs
StatePublished - 1995

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Cytoplasmic Dyneins
Transport Vesicles
Cytoskeleton
Microtubules
Rats
Hepatocytes
Ligands
Kinesin
Endocytosis
Adenosine Triphosphate
Dyneins
Endosomes
Lysosomes
Sorting
Immunoprecipitation

ASJC Scopus subject areas

  • Biochemistry

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Interaction of the microtubule cytoskeleton with endocytic vesicles and cytoplasmic dynein in cultured rat hepatocytes. / Oda, H.; Stockert, R. J.; Collins, C.; Wang, H.; Novikoff, P. M.; Satir, P.; Wolkoff, Allan W.

In: Journal of Biological Chemistry, Vol. 270, No. 25, 1995, p. 15242-15249.

Research output: Contribution to journalArticle

Oda, H. ; Stockert, R. J. ; Collins, C. ; Wang, H. ; Novikoff, P. M. ; Satir, P. ; Wolkoff, Allan W. / Interaction of the microtubule cytoskeleton with endocytic vesicles and cytoplasmic dynein in cultured rat hepatocytes. In: Journal of Biological Chemistry. 1995 ; Vol. 270, No. 25. pp. 15242-15249.
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