Interaction of the glycocalyx with the actin cytoskeleton

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Scopus citations

Abstract

In this chapter, we summarize and describe various methods that have been used to detect glycocalyx and to study the interactions between glycocalyx and actin cytoskeleton with the emphasis in the nervous tissue. We highlight two methodologies to analyze the direct interaction between glycocalyx and actin cytoskeleton: one method focusing on visualization using immunostaining and confocal microscopy techniques and the other focusing on direct protein-protein interactions using co-immunoprecipitation techniques.

Original languageEnglish (US)
Title of host publicationThe Cytoskeleton
Subtitle of host publicationImaging, Isolation, and Interaction
Pages43-62
Number of pages20
DOIs
StatePublished - Apr 2 2013

Publication series

NameNeuromethods
Volume79
ISSN (Print)0893-2336
ISSN (Electronic)1940-6045

Keywords

  • Actin cytoskeleton
  • Astrocyte
  • Chondroitin sulfate
  • Heparan sulfate
  • Hyaluronic acid

ASJC Scopus subject areas

  • Neuroscience(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Pharmacology, Toxicology and Pharmaceutics(all)
  • Psychiatry and Mental health

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  • Cite this

    Thi, M. M., Ebong, E. E., Spray, D. C., & Suadicani, S. O. (2013). Interaction of the glycocalyx with the actin cytoskeleton. In The Cytoskeleton: Imaging, Isolation, and Interaction (pp. 43-62). (Neuromethods; Vol. 79). https://doi.org/10.1007/978-1-62703-266-7-3