Interaction of the glycocalyx with the actin cytoskeleton

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Citation (Scopus)

Abstract

In this chapter, we summarize and describe various methods that have been used to detect glycocalyx and to study the interactions between glycocalyx and actin cytoskeleton with the emphasis in the nervous tissue. We highlight two methodologies to analyze the direct interaction between glycocalyx and actin cytoskeleton: one method focusing on visualization using immunostaining and confocal microscopy techniques and the other focusing on direct protein-protein interactions using co-immunoprecipitation techniques.

Original languageEnglish (US)
Title of host publicationNeuromethods
Pages43-62
Number of pages20
Volume79
DOIs
StatePublished - 2013

Publication series

NameNeuromethods
Volume79
ISSN (Print)08932336
ISSN (Electronic)19406045

Fingerprint

Glycocalyx
Actin Cytoskeleton
Actins
Confocal microscopy
Nerve Tissue
Proteins
Visualization
Tissue
Immunoprecipitation
Confocal Microscopy

Keywords

  • Actin cytoskeleton
  • Astrocyte
  • Chondroitin sulfate
  • Heparan sulfate
  • Hyaluronic acid

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Pharmacology, Toxicology and Pharmaceutics(all)
  • Neuroscience(all)
  • Psychiatry and Mental health

Cite this

Interaction of the glycocalyx with the actin cytoskeleton. / Thi, Mia M.; Ebong, Eno E.; Spray, David C.; Suadicani, Sylvia O.

Neuromethods. Vol. 79 2013. p. 43-62 (Neuromethods; Vol. 79).

Research output: Chapter in Book/Report/Conference proceedingChapter

Thi, Mia M. ; Ebong, Eno E. ; Spray, David C. ; Suadicani, Sylvia O. / Interaction of the glycocalyx with the actin cytoskeleton. Neuromethods. Vol. 79 2013. pp. 43-62 (Neuromethods).
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