Interaction of outer envelope proteins of Chlamydia psittaci GPIC with the HeLa cell surface

L. M. Ting, R. C. Hsia, C. G. Haidaris, P. M. Bavoil

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

The chlamydial life cycle involves the intimate interaction of components of the infectious elementary body (EB) surface with receptors on the susceptible eukaryotic cell plasma membrane. We have developed an in vitro ligand binding assay system for the identification and characterization of detergent-extracted EB envelope proteins capable of binding to glutaraldehyde-fixed HeLa cell surfaces. With this assay, the developmentally regulated cysteine-rich envelope protein Omp2 of Chlamydia psittaci strain guinea pig inclusion conjunctivitis was shown to bind specifically to HeLa cells. HeLa cells bound Omp2 selectively over other cell wall-associated proteins, including the major outer membrane protein, and the binding of Omp2 was abolished under conditions which alter its conformation. Furthermore, trypsin treatment, which reduces EB adherence, resulted in the proteolytic removal of a small terminal peptide of Omp2 at the EB surface and inactivated Omp2 in the ligand binding assay, while having a negligible effect on the major outer membrane protein. Collectively, our results suggest that Omp2 possesses the capacity to engage in a specific interaction with the host eukaryotic cell. We speculate that, since Omp2 is present only in the infectious EB form, the observed in vitro interaction may be representative of a determining step of the chlamydial pathogenic process.

Original languageEnglish (US)
Pages (from-to)3600-3608
Number of pages9
JournalInfection and Immunity
Volume63
Issue number9
StatePublished - 1995
Externally publishedYes

Fingerprint

Chlamydophila psittaci
HeLa Cells
Eukaryotic Cells
Protein Binding
Inclusion Conjunctivitis
Membrane Proteins
Cell Membrane
Ligands
Proteins
Glutaral
Life Cycle Stages
Detergents
Trypsin
Cell Wall
Cysteine
Guinea Pigs
Peptides
In Vitro Techniques

ASJC Scopus subject areas

  • Immunology

Cite this

Ting, L. M., Hsia, R. C., Haidaris, C. G., & Bavoil, P. M. (1995). Interaction of outer envelope proteins of Chlamydia psittaci GPIC with the HeLa cell surface. Infection and Immunity, 63(9), 3600-3608.

Interaction of outer envelope proteins of Chlamydia psittaci GPIC with the HeLa cell surface. / Ting, L. M.; Hsia, R. C.; Haidaris, C. G.; Bavoil, P. M.

In: Infection and Immunity, Vol. 63, No. 9, 1995, p. 3600-3608.

Research output: Contribution to journalArticle

Ting, LM, Hsia, RC, Haidaris, CG & Bavoil, PM 1995, 'Interaction of outer envelope proteins of Chlamydia psittaci GPIC with the HeLa cell surface', Infection and Immunity, vol. 63, no. 9, pp. 3600-3608.
Ting, L. M. ; Hsia, R. C. ; Haidaris, C. G. ; Bavoil, P. M. / Interaction of outer envelope proteins of Chlamydia psittaci GPIC with the HeLa cell surface. In: Infection and Immunity. 1995 ; Vol. 63, No. 9. pp. 3600-3608.
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