Interaction of hyperthermia and pentamidine in HeLa S-3 cells

S. H. Kim, S. S. Hong, A. A. Alfieri, J. H. Kim

Research output: Contribution to journalArticle

Abstract

Pentamidine is similar to rhodamine-123 in chemical structure and state of electron charge, and rhodamine-123 was previously shown to be a hyperthermic sensitizer under appropriate cell culture conditions. The present experiments were carried out to determine whether pentamidine would potentiate hyperthermic cell killing and, if so, under what cultural conditions. Exposure of HeLa cells to pentamidine (80 μM) up to 4 h was not cytotoxic in culture medium in the presence or absence of glucose at 37°C and pH 7.4. Cells in the glucose-deprived medium became progressively sensitive to killing as temperature and drug concentration were increased. On the other hand, there was a moderate level of enhanced cell killing in the glucose-fed medium at 42°C. The enhanced effects of heat by the drug were most pronounced under alkaline pH of the culture medium. The cell kill under acidic pH was far less than that observed at neutral or alkaline pH; these effects may be a result of decreased cellular uptake of pentamidine. Together with our previous results on rhodamine-123 and glycolytic inhibitors, the present data with pentamidine are consistent with the concept that deprivation of cellular energy increases sensitivity to cell killing by heat.

Original languageEnglish (US)
Pages (from-to)320-326
Number of pages7
JournalRadiation Research
Volume116
Issue number2
StatePublished - 1988
Externally publishedYes

Fingerprint

Pentamidine
hyperthermia
fever
Rhodamine 123
Fever
rhodamine
glucose
culture media
cells
drugs
Glucose
interactions
Culture Media
deprivation
heat
Hot Temperature
inhibitors
HeLa Cells
Pharmaceutical Preparations
chemical structure

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biophysics
  • Radiology Nuclear Medicine and imaging
  • Radiation

Cite this

Kim, S. H., Hong, S. S., Alfieri, A. A., & Kim, J. H. (1988). Interaction of hyperthermia and pentamidine in HeLa S-3 cells. Radiation Research, 116(2), 320-326.

Interaction of hyperthermia and pentamidine in HeLa S-3 cells. / Kim, S. H.; Hong, S. S.; Alfieri, A. A.; Kim, J. H.

In: Radiation Research, Vol. 116, No. 2, 1988, p. 320-326.

Research output: Contribution to journalArticle

Kim, SH, Hong, SS, Alfieri, AA & Kim, JH 1988, 'Interaction of hyperthermia and pentamidine in HeLa S-3 cells', Radiation Research, vol. 116, no. 2, pp. 320-326.
Kim SH, Hong SS, Alfieri AA, Kim JH. Interaction of hyperthermia and pentamidine in HeLa S-3 cells. Radiation Research. 1988;116(2):320-326.
Kim, S. H. ; Hong, S. S. ; Alfieri, A. A. ; Kim, J. H. / Interaction of hyperthermia and pentamidine in HeLa S-3 cells. In: Radiation Research. 1988 ; Vol. 116, No. 2. pp. 320-326.
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