Interaction of extracellular matrix and activin-A in the initiation of follicle growth in the mouse ovary

K. Oktay, G. Karlikaya, O. Akman, G. K. Ojakian, Maja H. Oktay

Research output: Contribution to journalArticle

52 Citations (Scopus)

Abstract

The precise mechanism for the initiation of follicle growth and progression through the earliest stages of follicle development remains largely unknown. Activins play a role during early follicle development, and evidence suggests that the extracellular matrix plays a role during later stages of follicular growth. We investigated the role of activin-A and extracellular matrix in follicle growth initiation and early follicular development in the mouse ovary. Ovaries were collected from 5-day-old mice and cultured for 10 days on polylysine, collagen, or laminin in the presence or absence of recombinant human activin-A. Follicle density, indices of follicle growth initiation (primary:primordial follicle [PY:PD] and primary:total follicle [PY:TF] ratios), ratios of multilayer follicle:total follicle (ML:TF), and follicle growth rates were compared between groups. Follicle densities were significantly higher in the extracellular matrix treatment group compared with the polylysine group (P < 0.01). Also, compared with polylysine, both collagen and laminin significantly increased indices of follicle growth initiation (PY:PD ratio: P < 0.001, odds ratio of 3.3; PY:TF ratio: P < 0.001, odds ratio of 2.5), and these were not altered by activin treatment. In the absence of activin-A, exposure to neither collagen nor laminin had an effect on multilayer follicle development. When activin-A was added, collagen and laminin had opposing effects on multilayer follicle development. Activin-A stimulated multilayer follicle development in the presence of laminin (ML:TF ratio: P = 0.01, odds ratio of 10.8), whereas it suppressed follicle growth in collagen (P = 0.01). Activin-A did not affect the ML:TF Ratio in the polylysine-treated groups. These results strongly suggest that extracellular matrix components and activin-A interact with each other, and that they regulate follicle growth initiation and multilayer follicle development.

Original languageEnglish (US)
Pages (from-to)457-461
Number of pages5
JournalBiology of Reproduction
Volume63
Issue number2
StatePublished - 2000
Externally publishedYes

Fingerprint

Extracellular Matrix
Ovary
Laminin
Polylysine
Growth
Collagen
Activins
Odds Ratio
activin A

Keywords

  • Activin
  • Follicle
  • Follicular development
  • FSH

ASJC Scopus subject areas

  • Cell Biology
  • Developmental Biology
  • Embryology

Cite this

Interaction of extracellular matrix and activin-A in the initiation of follicle growth in the mouse ovary. / Oktay, K.; Karlikaya, G.; Akman, O.; Ojakian, G. K.; Oktay, Maja H.

In: Biology of Reproduction, Vol. 63, No. 2, 2000, p. 457-461.

Research output: Contribution to journalArticle

Oktay, K. ; Karlikaya, G. ; Akman, O. ; Ojakian, G. K. ; Oktay, Maja H. / Interaction of extracellular matrix and activin-A in the initiation of follicle growth in the mouse ovary. In: Biology of Reproduction. 2000 ; Vol. 63, No. 2. pp. 457-461.
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