Interaction of diphtheria toxin B subunit with sensitive and insensitive mammalian cells

A. J. Labyntsev, N. V. Korotkevich, Andrii Kaberniuk, S. I. Romaniuk, D. V. Kolibo, S. V. Komisarenko

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The recombinant fluorescent derivative of diphtheria toxin (EGFP-SbB) obtained by the replacement of toxin A subunit by enhanced green fluorescent protein (EGFP) has been used for visualization of the interaction of diphtheria toxin (DT) with sensitive and insensitive cells. It was shown that EGFP-SbB could interact with cell surface of both toxin-sensitive monkey cells (Vero cell line) and toxin-resistant mouse cells (3T3 cell line). The affinity of this protein for receptors of Vero cells was three times higher as compared with 3T3 cells. It was demonstrated that fluorescent derivate was able to interact with receptors of both cell lines and to internalize into these cells. Internalization of EGFP-SbB into the cells was inhibited by endocytosis inhibitor phenyl arsine oxide. We suppose that diverse sensitivity to DT of monkey and mouse cells can be explained not only by differences in their receptor affinity for DT but also by the processes that occur after internalization of the toxin into the cells.

Original languageEnglish (US)
Pages (from-to)65-75
Number of pages11
JournalUkrain'skyi Biokhimichnyi Zhurnal
Volume82
Issue number6
StatePublished - 2010
Externally publishedYes

Fingerprint

Diphtheria Toxin
Cells
3T3 Cells
Vero Cells
Cell Line
Oxides
Haplorhini
Visualization
Shiga Toxins
Derivatives
enhanced green fluorescent protein
Endocytosis
Proteins

Keywords

  • B subunit of diphtheria toxin
  • Endocytosis
  • Fluorescent derivates
  • Receptor binding
  • Toxin-sensitive and toxinresistant cells

ASJC Scopus subject areas

  • Biochemistry

Cite this

Labyntsev, A. J., Korotkevich, N. V., Kaberniuk, A., Romaniuk, S. I., Kolibo, D. V., & Komisarenko, S. V. (2010). Interaction of diphtheria toxin B subunit with sensitive and insensitive mammalian cells. Ukrain'skyi Biokhimichnyi Zhurnal, 82(6), 65-75.

Interaction of diphtheria toxin B subunit with sensitive and insensitive mammalian cells. / Labyntsev, A. J.; Korotkevich, N. V.; Kaberniuk, Andrii; Romaniuk, S. I.; Kolibo, D. V.; Komisarenko, S. V.

In: Ukrain'skyi Biokhimichnyi Zhurnal, Vol. 82, No. 6, 2010, p. 65-75.

Research output: Contribution to journalArticle

Labyntsev, AJ, Korotkevich, NV, Kaberniuk, A, Romaniuk, SI, Kolibo, DV & Komisarenko, SV 2010, 'Interaction of diphtheria toxin B subunit with sensitive and insensitive mammalian cells', Ukrain'skyi Biokhimichnyi Zhurnal, vol. 82, no. 6, pp. 65-75.
Labyntsev AJ, Korotkevich NV, Kaberniuk A, Romaniuk SI, Kolibo DV, Komisarenko SV. Interaction of diphtheria toxin B subunit with sensitive and insensitive mammalian cells. Ukrain'skyi Biokhimichnyi Zhurnal. 2010;82(6):65-75.
Labyntsev, A. J. ; Korotkevich, N. V. ; Kaberniuk, Andrii ; Romaniuk, S. I. ; Kolibo, D. V. ; Komisarenko, S. V. / Interaction of diphtheria toxin B subunit with sensitive and insensitive mammalian cells. In: Ukrain'skyi Biokhimichnyi Zhurnal. 2010 ; Vol. 82, No. 6. pp. 65-75.
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