TY - JOUR
T1 - Interaction between a complex of RNA polymerase III subunits and the 70-kDa component of transcription factor IIIB
AU - Werner, Michel
AU - Chaussivert, Nathalie
AU - Willis, Ian M.
AU - Sentenac, André
N1 - Copyright:
Copyright 2004 Elsevier B.V., All rights reserved.
PY - 1993/10/5
Y1 - 1993/10/5
N2 - A system that detects the formation of complexes between different proteins by linking them to separate domains of the GAL4 transcription activator protein has been used to study protein-protein interactions between four essential and unique subunits of yeast RNA polymerase III (C82, C53, C34 and C31), the 70-kDa component of the initiation transcription factor IIIB (TFIIIB70) and the TATA-binding protein. We found that C82, C34, and C31 are able to combine with each other in vivo and that C34 interacts with TFIIIB70. These results suggest that C34 and TFIIIB70 are specificity determinants of the RNA polymerase III-TFIIIB interaction.
AB - A system that detects the formation of complexes between different proteins by linking them to separate domains of the GAL4 transcription activator protein has been used to study protein-protein interactions between four essential and unique subunits of yeast RNA polymerase III (C82, C53, C34 and C31), the 70-kDa component of the initiation transcription factor IIIB (TFIIIB70) and the TATA-binding protein. We found that C82, C34, and C31 are able to combine with each other in vivo and that C34 interacts with TFIIIB70. These results suggest that C34 and TFIIIB70 are specificity determinants of the RNA polymerase III-TFIIIB interaction.
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M3 - Article
C2 - 8407894
AN - SCOPUS:0027485935
SN - 0021-9258
VL - 268
SP - 20721
EP - 20724
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
IS - 28
ER -