Interaction between a complex of RNA polymerase III subunits and the 70-kDa component of transcription factor IIIB

Michel Werner, Nathalie Chaussivert, Ian M. Willis, André Sentenac

Research output: Contribution to journalArticle

102 Citations (Scopus)

Abstract

A system that detects the formation of complexes between different proteins by linking them to separate domains of the GAL4 transcription activator protein has been used to study protein-protein interactions between four essential and unique subunits of yeast RNA polymerase III (C82, C53, C34 and C31), the 70-kDa component of the initiation transcription factor IIIB (TFIIIB70) and the TATA-binding protein. We found that C82, C34, and C31 are able to combine with each other in vivo and that C34 interacts with TFIIIB70. These results suggest that C34 and TFIIIB70 are specificity determinants of the RNA polymerase III-TFIIIB interaction.

Original languageEnglish (US)
Pages (from-to)20721-20724
Number of pages4
JournalJournal of Biological Chemistry
Volume268
Issue number28
StatePublished - Oct 5 1993

Fingerprint

Transcription Factor TFIIIB
RNA Polymerase III
Proteins
TATA-Box Binding Protein
Peptide Initiation Factors
Transcription
Yeast
Yeasts

ASJC Scopus subject areas

  • Biochemistry

Cite this

Interaction between a complex of RNA polymerase III subunits and the 70-kDa component of transcription factor IIIB. / Werner, Michel; Chaussivert, Nathalie; Willis, Ian M.; Sentenac, André.

In: Journal of Biological Chemistry, Vol. 268, No. 28, 05.10.1993, p. 20721-20724.

Research output: Contribution to journalArticle

Werner, Michel ; Chaussivert, Nathalie ; Willis, Ian M. ; Sentenac, André. / Interaction between a complex of RNA polymerase III subunits and the 70-kDa component of transcription factor IIIB. In: Journal of Biological Chemistry. 1993 ; Vol. 268, No. 28. pp. 20721-20724.
@article{6df5a0a56b6d4a20a01900e01d7b0132,
title = "Interaction between a complex of RNA polymerase III subunits and the 70-kDa component of transcription factor IIIB",
abstract = "A system that detects the formation of complexes between different proteins by linking them to separate domains of the GAL4 transcription activator protein has been used to study protein-protein interactions between four essential and unique subunits of yeast RNA polymerase III (C82, C53, C34 and C31), the 70-kDa component of the initiation transcription factor IIIB (TFIIIB70) and the TATA-binding protein. We found that C82, C34, and C31 are able to combine with each other in vivo and that C34 interacts with TFIIIB70. These results suggest that C34 and TFIIIB70 are specificity determinants of the RNA polymerase III-TFIIIB interaction.",
author = "Michel Werner and Nathalie Chaussivert and Willis, {Ian M.} and Andr{\'e} Sentenac",
year = "1993",
month = "10",
day = "5",
language = "English (US)",
volume = "268",
pages = "20721--20724",
journal = "Journal of Biological Chemistry",
issn = "0021-9258",
publisher = "American Society for Biochemistry and Molecular Biology Inc.",
number = "28",

}

TY - JOUR

T1 - Interaction between a complex of RNA polymerase III subunits and the 70-kDa component of transcription factor IIIB

AU - Werner, Michel

AU - Chaussivert, Nathalie

AU - Willis, Ian M.

AU - Sentenac, André

PY - 1993/10/5

Y1 - 1993/10/5

N2 - A system that detects the formation of complexes between different proteins by linking them to separate domains of the GAL4 transcription activator protein has been used to study protein-protein interactions between four essential and unique subunits of yeast RNA polymerase III (C82, C53, C34 and C31), the 70-kDa component of the initiation transcription factor IIIB (TFIIIB70) and the TATA-binding protein. We found that C82, C34, and C31 are able to combine with each other in vivo and that C34 interacts with TFIIIB70. These results suggest that C34 and TFIIIB70 are specificity determinants of the RNA polymerase III-TFIIIB interaction.

AB - A system that detects the formation of complexes between different proteins by linking them to separate domains of the GAL4 transcription activator protein has been used to study protein-protein interactions between four essential and unique subunits of yeast RNA polymerase III (C82, C53, C34 and C31), the 70-kDa component of the initiation transcription factor IIIB (TFIIIB70) and the TATA-binding protein. We found that C82, C34, and C31 are able to combine with each other in vivo and that C34 interacts with TFIIIB70. These results suggest that C34 and TFIIIB70 are specificity determinants of the RNA polymerase III-TFIIIB interaction.

UR - http://www.scopus.com/inward/record.url?scp=0027485935&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027485935&partnerID=8YFLogxK

M3 - Article

VL - 268

SP - 20721

EP - 20724

JO - Journal of Biological Chemistry

JF - Journal of Biological Chemistry

SN - 0021-9258

IS - 28

ER -