Interacting QTLs for cholesterol gallstones and gallbladder mucin in AKR and SWR strains of mice.

Henning Wittenburg, Frank Lammert, David Q.H. Wang, Gary A. Churchill, Renhua Li, Guylaine Bouchard, Martin C. Carey, Beverly Paigen

Research output: Contribution to journalArticle

Abstract

We employed quantitative trait locus (QTL) mapping in a backcross between gallstone-susceptible SWR/J and gallstone-resistant AKR/J inbred mice to identify additional susceptibility loci for cholesterol gallstone formation. After 12 wk of feeding the mice a lithogenic diet, we phenotyped 330 backcross progeny for gallstones, gallbladder mucin accumulation, liver weight, and body weight. Marker-based regression analysis revealed significant single QTLs associated with gallstone formation on chromosome 9 and the liver weight/body weight ratio on chromosomes 5 and X. A search for gene pairs detected significant gene-gene interactions for mucin accumulation between loci on chromosomes 5 and 11 and suggestive gene-gene interactions linked to gallstone formation between the QTL on chromosome 9 and loci on chromosomes 6 and 15. These findings uncover new QTLs for cholesterol gallstones, reveal independent loci for mucin accumulation, and demonstrate the importance of considering gene-gene interactions in cholesterol cholelithiasis. According to standard nomenclature, the gallstone QTL on chromosome 9 is named Lith5.

Original languageEnglish (US)
Pages (from-to)67-77
Number of pages11
JournalPhysiological Genomics
Volume8
Issue number1
DOIs
StatePublished - Jan 1 2002
Externally publishedYes

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Gallstones
Mucins
Gallbladder
Cholesterol
Chromosomes, Human, Pair 9
Quantitative Trait Loci
Genes
Chromosomes, Human, Pair 5
Body Weight
Inbred AKR Mouse
Chromosomes, Human, Pair 15
Weights and Measures
Chromosomes, Human, Pair 11
Chromosomes, Human, Pair 6
Cholelithiasis
Liver
Terminology
Regression Analysis
Diet

ASJC Scopus subject areas

  • Physiology
  • Genetics

Cite this

Interacting QTLs for cholesterol gallstones and gallbladder mucin in AKR and SWR strains of mice. / Wittenburg, Henning; Lammert, Frank; Wang, David Q.H.; Churchill, Gary A.; Li, Renhua; Bouchard, Guylaine; Carey, Martin C.; Paigen, Beverly.

In: Physiological Genomics, Vol. 8, No. 1, 01.01.2002, p. 67-77.

Research output: Contribution to journalArticle

Wittenburg, H, Lammert, F, Wang, DQH, Churchill, GA, Li, R, Bouchard, G, Carey, MC & Paigen, B 2002, 'Interacting QTLs for cholesterol gallstones and gallbladder mucin in AKR and SWR strains of mice.', Physiological Genomics, vol. 8, no. 1, pp. 67-77. https://doi.org/10.1152/physiolgenomics.00097.2001
Wittenburg, Henning ; Lammert, Frank ; Wang, David Q.H. ; Churchill, Gary A. ; Li, Renhua ; Bouchard, Guylaine ; Carey, Martin C. ; Paigen, Beverly. / Interacting QTLs for cholesterol gallstones and gallbladder mucin in AKR and SWR strains of mice. In: Physiological Genomics. 2002 ; Vol. 8, No. 1. pp. 67-77.
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