Insulin-treated 3T3-L1 adipocytes and cell-free extracts derived from them incorporate 32P into ribsomal protein S6

C. J. Smith, C. S. Rubin, O. Mendelsohn Rosen

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

Differentiated 3T3-L1 preadipocytes preincubated for 60 min with 32Pi incorporate 32P into ribosomal protein S6 within 5 min after exposure to 0.1-1.0 nM insulin. Undifferentiated 3T3-L1 cells, which possess only 3-5% of the high-affinity cell surface insulin receptors present on the differentiated cells, are less sensitive to this stimulation. Under the same conditions used for insulin, epidermal growth factor, antibody to the insulin receptor, and a combination of isoproterenol and 1-methyl-3-isobutylxanthine also promote 32P incorporation into S6 in the differentiated cells although less effectively than insulin. Cell-free extracts derived from cells treated for 5-10 min with either physiological concentrations of insulin or epidermal growth factor (0.1 μg/ml) reflect intact cells and catalyze the incorporation of 32P from exogenous [γ-32P]ATP into ribosomal protein S6.

Original languageEnglish (US)
Pages (from-to)2641-2645
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume77
Issue number5 I
StatePublished - 1980

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3T3-L1 Cells
S 6
Cell Extracts
Adipocytes
Ribosomal Protein S6
Insulin
Insulin Receptor
Epidermal Growth Factor
Proteins
1-Methyl-3-isobutylxanthine
Cell Surface Receptors
Isoproterenol
Adenosine Triphosphate
Antibodies

ASJC Scopus subject areas

  • General
  • Genetics

Cite this

Insulin-treated 3T3-L1 adipocytes and cell-free extracts derived from them incorporate 32P into ribsomal protein S6. / Smith, C. J.; Rubin, C. S.; Mendelsohn Rosen, O.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 77, No. 5 I, 1980, p. 2641-2645.

Research output: Contribution to journalArticle

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