Insulin-stimulated sodium transport in toad urinary bladder

Melanie H. Cobb, Chia-Ping H. Yang, James A. Brown, Walter N. Scott

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Mammalian and teleost insulins increase active sodium transport by the toad urinary bladder at subnanomolar concentrations. This stimulation is evident within 15 min and persists for hours. Porcine proinsulin and a cross-linked derivative of bovine insulin are less effective than porcine insulin in stimulating the short-circuit current (SCC), indicating the specificity appropriate for activation of sodium transport through an insulin receptor. The initial stimulation by insulin of the SCC is not blocked by pretreatment with actinomycin D, puromycin, cycloheximide, or tunicamycin. However, in the presence of any one of these inhibitors the sustained increase in SCC is blocked and the rise is short-lived, lasting only 45 to 90 min. In amphotericin-treated bladders, the addition of insulin did not further stimulate SCC.

Original languageEnglish (US)
Pages (from-to)123-129
Number of pages7
JournalBBA - Biomembranes
Volume856
Issue number1
DOIs
StatePublished - Mar 27 1986
Externally publishedYes

Fingerprint

Short circuit currents
Anura
Urinary Bladder
Sodium
Insulin
Insulin, Regular, Pork
Insulins
Proinsulin
Tunicamycin
Puromycin
Active Biological Transport
Insulin Receptor
Dactinomycin
Amphotericin B
Cycloheximide
Swine
Chemical activation
Derivatives

Keywords

  • (Toad urinary bladder)
  • Insulin
  • Na transport
  • Protein synthesis

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology

Cite this

Insulin-stimulated sodium transport in toad urinary bladder. / Cobb, Melanie H.; Yang, Chia-Ping H.; Brown, James A.; Scott, Walter N.

In: BBA - Biomembranes, Vol. 856, No. 1, 27.03.1986, p. 123-129.

Research output: Contribution to journalArticle

Cobb, Melanie H. ; Yang, Chia-Ping H. ; Brown, James A. ; Scott, Walter N. / Insulin-stimulated sodium transport in toad urinary bladder. In: BBA - Biomembranes. 1986 ; Vol. 856, No. 1. pp. 123-129.
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