Insulin-stimulated sodium transport in toad urinary bladder

Melanie H. Cobb, Chia Ping H. Yang, James A. Brown, Walter N. Scott

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

Mammalian and teleost insulins increase active sodium transport by the toad urinary bladder at subnanomolar concentrations. This stimulation is evident within 15 min and persists for hours. Porcine proinsulin and a cross-linked derivative of bovine insulin are less effective than porcine insulin in stimulating the short-circuit current (SCC), indicating the specificity appropriate for activation of sodium transport through an insulin receptor. The initial stimulation by insulin of the SCC is not blocked by pretreatment with actinomycin D, puromycin, cycloheximide, or tunicamycin. However, in the presence of any one of these inhibitors the sustained increase in SCC is blocked and the rise is short-lived, lasting only 45 to 90 min. In amphotericin-treated bladders, the addition of insulin did not further stimulate SCC.

Original languageEnglish (US)
Pages (from-to)123-129
Number of pages7
JournalBBA - Biomembranes
Volume856
Issue number1
DOIs
StatePublished - Mar 27 1986
Externally publishedYes

Keywords

  • (Toad urinary bladder)
  • Insulin
  • Na transport
  • Protein synthesis

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Cell Biology

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