Insulin and nonhydrolyzable GTP analogs induce translocation of GLUT 4 to the plasma membrane in α-toxin-permeabilized rat adipose cells

Giulia Baldini, Robert Hohman, Maureen J. Charron, Harvey F. Lodish

Research output: Contribution to journalArticle

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Abstract

Rat adipose cells treated with Staphylococcus aureus α-toxin are permeable and retain their ability to respond to insulin after hormone treatment. The GLUT 4 glucose transporter isoform, specific to fat and muscle cells, is translocated normally from low density microsomes to the plasma membrane in permeabilized cells. Addition of guanosine 5′-O-(3-thiotriphosphate), guanylyl imidodiphosphate, or guanylyl β,γ-methylenediphosphate to permeabilized adipocytes induces an insulin-like translocation of GLUT 4 to the plasma membrane; GTP or adenosine 5′-(β,γ-imino)triphosphate has no effect. No translocation of GLUT 4 is observed when GTP analogs are added to intact adipocytes. These results suggest the involvement of a GTP-binding protein in insulin-triggered recruitment of GLUT 4 to the cell surface.

Original languageEnglish (US)
Pages (from-to)4037-4040
Number of pages4
JournalJournal of Biological Chemistry
Volume266
Issue number7
StatePublished - Mar 5 1991

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Cell membranes
Guanosine Triphosphate
Adipocytes
Rats
Cell Membrane
Insulin
Guanylyl Imidodiphosphate
Guanosine 5'-O-(3-Thiotriphosphate)
Facilitative Glucose Transport Proteins
Microsomes
GTP-Binding Proteins
Adenosine
Muscle Cells
Muscle
Staphylococcus aureus
Protein Isoforms
Fats
Cells
Hormones

ASJC Scopus subject areas

  • Biochemistry

Cite this

Insulin and nonhydrolyzable GTP analogs induce translocation of GLUT 4 to the plasma membrane in α-toxin-permeabilized rat adipose cells. / Baldini, Giulia; Hohman, Robert; Charron, Maureen J.; Lodish, Harvey F.

In: Journal of Biological Chemistry, Vol. 266, No. 7, 05.03.1991, p. 4037-4040.

Research output: Contribution to journalArticle

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