Instability of immunoglobulin genes in S107 cell line

Seung Uon Shin, Ronald DePinho, Donald J. Zack, Stuart Rudikoff, Matthew D. Scharff

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Somatic mutation occurs frequently in rearranged and expressed immunoglobulin variable region genes in vivo. In contrast, V region hypermutation seldom occurs in antibody-forming cells in culture. The S107 mouse myeloma cell line is one of the few cell lines that has been observed to generate V region mutations frequently and spontaneously in vitro. Detailed examination reveals that both the S107 tumor and the cell line derived from it contain and express a duplicated heavy-chain gene. In culture, only one of the two heavy-chain genes undergoes both V and C region mutation, and variants with complex phenotypes and genotypes arise as a result of mutation and segregation of these duplicated genes.

Original languageEnglish (US)
Pages (from-to)259-276
Number of pages18
JournalSomatic Cell and Molecular Genetics
Volume17
Issue number3
DOIs
StatePublished - May 1991

Fingerprint

Immunoglobulin Genes
Cell Line
Mutation
Genes
Immunoglobulin Variable Region
Tumor Cell Line
Cell Culture Techniques
Genotype
Phenotype
Antibodies

ASJC Scopus subject areas

  • Genetics
  • Cell Biology

Cite this

Instability of immunoglobulin genes in S107 cell line. / Shin, Seung Uon; DePinho, Ronald; Zack, Donald J.; Rudikoff, Stuart; Scharff, Matthew D.

In: Somatic Cell and Molecular Genetics, Vol. 17, No. 3, 05.1991, p. 259-276.

Research output: Contribution to journalArticle

Shin, Seung Uon ; DePinho, Ronald ; Zack, Donald J. ; Rudikoff, Stuart ; Scharff, Matthew D. / Instability of immunoglobulin genes in S107 cell line. In: Somatic Cell and Molecular Genetics. 1991 ; Vol. 17, No. 3. pp. 259-276.
@article{ada78a53ad454eb885d35fcec447373f,
title = "Instability of immunoglobulin genes in S107 cell line",
abstract = "Somatic mutation occurs frequently in rearranged and expressed immunoglobulin variable region genes in vivo. In contrast, V region hypermutation seldom occurs in antibody-forming cells in culture. The S107 mouse myeloma cell line is one of the few cell lines that has been observed to generate V region mutations frequently and spontaneously in vitro. Detailed examination reveals that both the S107 tumor and the cell line derived from it contain and express a duplicated heavy-chain gene. In culture, only one of the two heavy-chain genes undergoes both V and C region mutation, and variants with complex phenotypes and genotypes arise as a result of mutation and segregation of these duplicated genes.",
author = "Shin, {Seung Uon} and Ronald DePinho and Zack, {Donald J.} and Stuart Rudikoff and Scharff, {Matthew D.}",
year = "1991",
month = "5",
doi = "10.1007/BF01232821",
language = "English (US)",
volume = "17",
pages = "259--276",
journal = "Somatic Cell and Molecular Genetics",
issn = "0740-7750",
publisher = "Springer GmbH & Co, Auslieferungs-Gesellschaf",
number = "3",

}

TY - JOUR

T1 - Instability of immunoglobulin genes in S107 cell line

AU - Shin, Seung Uon

AU - DePinho, Ronald

AU - Zack, Donald J.

AU - Rudikoff, Stuart

AU - Scharff, Matthew D.

PY - 1991/5

Y1 - 1991/5

N2 - Somatic mutation occurs frequently in rearranged and expressed immunoglobulin variable region genes in vivo. In contrast, V region hypermutation seldom occurs in antibody-forming cells in culture. The S107 mouse myeloma cell line is one of the few cell lines that has been observed to generate V region mutations frequently and spontaneously in vitro. Detailed examination reveals that both the S107 tumor and the cell line derived from it contain and express a duplicated heavy-chain gene. In culture, only one of the two heavy-chain genes undergoes both V and C region mutation, and variants with complex phenotypes and genotypes arise as a result of mutation and segregation of these duplicated genes.

AB - Somatic mutation occurs frequently in rearranged and expressed immunoglobulin variable region genes in vivo. In contrast, V region hypermutation seldom occurs in antibody-forming cells in culture. The S107 mouse myeloma cell line is one of the few cell lines that has been observed to generate V region mutations frequently and spontaneously in vitro. Detailed examination reveals that both the S107 tumor and the cell line derived from it contain and express a duplicated heavy-chain gene. In culture, only one of the two heavy-chain genes undergoes both V and C region mutation, and variants with complex phenotypes and genotypes arise as a result of mutation and segregation of these duplicated genes.

UR - http://www.scopus.com/inward/record.url?scp=0026332590&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026332590&partnerID=8YFLogxK

U2 - 10.1007/BF01232821

DO - 10.1007/BF01232821

M3 - Article

VL - 17

SP - 259

EP - 276

JO - Somatic Cell and Molecular Genetics

JF - Somatic Cell and Molecular Genetics

SN - 0740-7750

IS - 3

ER -