Instability of immunoglobulin genes in S107 cell line

Seung Uon Shin, Ronald DePinho, Donald J. Zack, Stuart Rudikoff, Matthew D. Scharff

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

Somatic mutation occurs frequently in rearranged and expressed immunoglobulin variable region genes in vivo. In contrast, V region hypermutation seldom occurs in antibody-forming cells in culture. The S107 mouse myeloma cell line is one of the few cell lines that has been observed to generate V region mutations frequently and spontaneously in vitro. Detailed examination reveals that both the S107 tumor and the cell line derived from it contain and express a duplicated heavy-chain gene. In culture, only one of the two heavy-chain genes undergoes both V and C region mutation, and variants with complex phenotypes and genotypes arise as a result of mutation and segregation of these duplicated genes.

Original languageEnglish (US)
Pages (from-to)259-276
Number of pages18
JournalSomatic Cell and Molecular Genetics
Volume17
Issue number3
DOIs
Publication statusPublished - May 1 1991

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ASJC Scopus subject areas

  • Genetics
  • Cell Biology

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