Insertional mutagenesis in mice deficient for p15Ink4b, p16 Ink4a, p21Cip1, and p27Kip1 reveals cancer gene interactions and correlations with tumor phenotypes

Jaap Kool, Anthony G. Uren, Carla P. Martins, Daoud Sie, Jeroen De Ridder, Geoffrey Turner, Miranda Van Uitert, Konstantin Matentzoglu, Wendy Lagcher, Paul Krimpenfort, Jules Gadiot, Colin Pritchard, Jack Lenz, Anders H. Lund, Jos Jonkers, Jane Rogers, David J. Adams, Lodewyk Wessels, Anton Berns, Maarten Van Lohuizen

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

The cyclin dependent kinase (CDK) inhibitors p15, p16, p21, and p27 are frequently deleted, silenced, or downregulated in many malignancies. Inactivation of CDK inhibitors predisposes mice to tumor development, showing that these genes function as tumor suppressors. Here, we describe high-throughput murine leukemia virus insertional mutagenesis screens in mice that are deficient for one or two CDK inhibitors. We retrieved 9,117 retroviral insertions from 476 lymphomas to define hundreds of loci that are mutated more frequently than expected by chance. Many of these loci are skewed toward a specific genetic context of predisposing germline and somatic mutations. We also found associations between these loci with gender, age of tumor onset, and lymphocyte lineage (B or T cell). Comparison of retroviral insertion sites with single nucleotide polymorphisms associated with chronic lymphocytic leukemia revealed a significant overlap between the datasets. Together, our findings highlight the importance of genetic context within large-scale mutation detection studies, and they show a novel use for insertional mutagenesis data in prioritizing disease-associated genes that emerge from genome-wide association studies.

Original languageEnglish (US)
Pages (from-to)530-531
Number of pages2
JournalCancer Research
Volume70
Issue number2
DOIs
StatePublished - Jan 15 2010

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Insertional Mutagenesis
Neoplasm Genes
Phenotype
Cyclin-Dependent Kinases
Cyclin-Dependent Kinase Inhibitor p15
Neoplasms
Cyclin-Dependent Kinase Inhibitor p16
Murine Leukemia Viruses
Germ-Line Mutation
Genome-Wide Association Study
B-Cell Chronic Lymphocytic Leukemia
Age of Onset
Genes
Single Nucleotide Polymorphism
Lymphoma
B-Lymphocytes
Down-Regulation
Lymphocytes
T-Lymphocytes
Mutation

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Insertional mutagenesis in mice deficient for p15Ink4b, p16 Ink4a, p21Cip1, and p27Kip1 reveals cancer gene interactions and correlations with tumor phenotypes. / Kool, Jaap; Uren, Anthony G.; Martins, Carla P.; Sie, Daoud; De Ridder, Jeroen; Turner, Geoffrey; Van Uitert, Miranda; Matentzoglu, Konstantin; Lagcher, Wendy; Krimpenfort, Paul; Gadiot, Jules; Pritchard, Colin; Lenz, Jack; Lund, Anders H.; Jonkers, Jos; Rogers, Jane; Adams, David J.; Wessels, Lodewyk; Berns, Anton; Van Lohuizen, Maarten.

In: Cancer Research, Vol. 70, No. 2, 15.01.2010, p. 530-531.

Research output: Contribution to journalArticle

Kool, J, Uren, AG, Martins, CP, Sie, D, De Ridder, J, Turner, G, Van Uitert, M, Matentzoglu, K, Lagcher, W, Krimpenfort, P, Gadiot, J, Pritchard, C, Lenz, J, Lund, AH, Jonkers, J, Rogers, J, Adams, DJ, Wessels, L, Berns, A & Van Lohuizen, M 2010, 'Insertional mutagenesis in mice deficient for p15Ink4b, p16 Ink4a, p21Cip1, and p27Kip1 reveals cancer gene interactions and correlations with tumor phenotypes', Cancer Research, vol. 70, no. 2, pp. 530-531. https://doi.org/10.1158/0008-5472.CAN-09-2736
Kool, Jaap ; Uren, Anthony G. ; Martins, Carla P. ; Sie, Daoud ; De Ridder, Jeroen ; Turner, Geoffrey ; Van Uitert, Miranda ; Matentzoglu, Konstantin ; Lagcher, Wendy ; Krimpenfort, Paul ; Gadiot, Jules ; Pritchard, Colin ; Lenz, Jack ; Lund, Anders H. ; Jonkers, Jos ; Rogers, Jane ; Adams, David J. ; Wessels, Lodewyk ; Berns, Anton ; Van Lohuizen, Maarten. / Insertional mutagenesis in mice deficient for p15Ink4b, p16 Ink4a, p21Cip1, and p27Kip1 reveals cancer gene interactions and correlations with tumor phenotypes. In: Cancer Research. 2010 ; Vol. 70, No. 2. pp. 530-531.
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