Insertion of an intracisternal A particle within the 5′-regulatory region of a gene encoding folate-binding protein in L1210 leukemia cells in response to low folate selection: Association with increased protein expression

Kevin E. Brigle, Eric H. Westin, Micah T. Houghton, I. David Goldman

Research output: Contribution to journalArticlepeer-review

23 Scopus citations

Abstract

We have previously described a subline of L1210 murine leukemia cells (LL1) selected in a low level of 5-formyltetrahydrofolate which overexpresses a membrane-bound folate-binding protein (FBP1) and exhibits a rearrangement at the locus encoding this protein Genomic clones containing the entire FBP1-encoding DNA from both L1210 and LL1 were isolated and characterized. Sequence analysis indicates that, with exception of the 5′-region, the FBP1-encoding locus in both cell lines is identical. The rearrangement in LL1 results from the insertion of an intracisternal A particle (IAP) in the head-to-head (antisense) orientation 72 base pairs (bp) upstream of the FBP1 ATG start codon. The IAP likely provides an alternative promoter for FBP1 expression which may produce a novel transcript with enhanced stability. Presence of the IAP appears to inactivate or relocate normal cis-acting regulatory sequences as expression of the FBP1 transcript in LL1 is not regulated by the folate status of the cell.

Original languageEnglish (US)
Pages (from-to)22351-22355
Number of pages5
JournalJournal of Biological Chemistry
Volume267
Issue number31
StatePublished - Nov 5 1992
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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