Initial mechanistic studies of antisense targeting in cells

Xinrong Liu, Kayoko Nakamura, Yi Wang, Yee Wang, Guozheng Liu, Jiang He, Hongliu Ding, Peiyuan Lu, Mary Rusckowski, Atsushi Kubo, Donald J. Hnatowich

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

The continued development of antisense targeting will require a better understanding of the mechanism. Methods: We performed initial studies of the mechanism of intracellular antisense targeting through measurements of in situ transcription, immunofluorescence, reverse transcription polymerase chain reaction (RT-PCR), 32P-labeled uridine-5′-triphosphate (α-32PUTP) incorporation, nuclear accumulations of 99mTc-labeled DNAs, and messenger RNA (mRNA) transcription rate. As reported earlier, an antisense DNA against the mdr1 mRNA coding for P-glycoprotein (Pgp) and its sense DNA control were used in KB-G2 (Pgp++) cells. Results: Definitive evidence for antisense targeting was obtained by in situ transcription showing complementary DNA elongation in cells exposed to antisense DNA, acting therefore as an intracellular PCR primer of mdr1 mRNA, but not in cells exposed to sense DNA. Immunofluorescence staining showed higher accumulations of antisense versus sense DNAs in KB-G2 cells. Transnuclear migration was con-firmed by higher accumulations in the nucleus compared with the cytoplasm in cells incubated with 99mTc-labeled antisense DNA. However, the observed specific accumulations of antisense DNAs of about 10 6 per cell over 10 h could not be explained by a feedback mechanism upregulating transcription in cells exposed to antisense DNA as no increase in mRNA levels was detected by both RT-PCR and 32P-UTP in these cells. To explore an alternative hypothesis, a novel approach using 99mTc-labeled antisense DNA as a probe of total mRNA from cells previously saturated with unlabeled antisense DNA was used to estimate the transcription rate. Compared with controls, mdr1 mRNA levels were found to be initially low after saturation and to recover at about 2,000 copies per minute per cell. If persistent, this transcription rate would provide 106 mRNAs in 10 h. Conclusion: The results of all studies are consistent with antisense as the mechanism of targeting. Though a feedback mechanism leading to upregulation of mRNA transcription is an unlikely explanation for the high specific accumulations, our results may be explained if antisense DNAs are targeting mdr1 mRNAs produced at high transcription rates. If the target is primarily pre-mRNA in the nucleus rather than mature mRNA in the cytoplasm, this would provide as well an explanation for the observed migration of 99mTc-labeled antisense DNA into the nucleus.

Original languageEnglish (US)
Pages (from-to)360-368
Number of pages9
JournalJournal of Nuclear Medicine
Volume47
Issue number2
StatePublished - Feb 1 2006
Externally publishedYes

Fingerprint

Antisense DNA
Messenger RNA
Uridine Triphosphate
DNA
P-Glycoprotein
Polymerase Chain Reaction
Reverse Transcription
Fluorescent Antibody Technique
Cytoplasm
KB Cells
Up-Regulation
Complementary DNA

Keywords

  • Antisense DNA
  • In situ transcription
  • mdr1 messenger RNA
  • Mechanism
  • Transcription rate

ASJC Scopus subject areas

  • Radiological and Ultrasound Technology

Cite this

Liu, X., Nakamura, K., Wang, Y., Wang, Y., Liu, G., He, J., ... Hnatowich, D. J. (2006). Initial mechanistic studies of antisense targeting in cells. Journal of Nuclear Medicine, 47(2), 360-368.

Initial mechanistic studies of antisense targeting in cells. / Liu, Xinrong; Nakamura, Kayoko; Wang, Yi; Wang, Yee; Liu, Guozheng; He, Jiang; Ding, Hongliu; Lu, Peiyuan; Rusckowski, Mary; Kubo, Atsushi; Hnatowich, Donald J.

In: Journal of Nuclear Medicine, Vol. 47, No. 2, 01.02.2006, p. 360-368.

Research output: Contribution to journalArticle

Liu, X, Nakamura, K, Wang, Y, Wang, Y, Liu, G, He, J, Ding, H, Lu, P, Rusckowski, M, Kubo, A & Hnatowich, DJ 2006, 'Initial mechanistic studies of antisense targeting in cells', Journal of Nuclear Medicine, vol. 47, no. 2, pp. 360-368.
Liu X, Nakamura K, Wang Y, Wang Y, Liu G, He J et al. Initial mechanistic studies of antisense targeting in cells. Journal of Nuclear Medicine. 2006 Feb 1;47(2):360-368.
Liu, Xinrong ; Nakamura, Kayoko ; Wang, Yi ; Wang, Yee ; Liu, Guozheng ; He, Jiang ; Ding, Hongliu ; Lu, Peiyuan ; Rusckowski, Mary ; Kubo, Atsushi ; Hnatowich, Donald J. / Initial mechanistic studies of antisense targeting in cells. In: Journal of Nuclear Medicine. 2006 ; Vol. 47, No. 2. pp. 360-368.
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