Initial association of NR2E1 with bipolar disorder and identification of candidate mutations in bipolar disorder, schizophrenia, and aggression through resequencing

Ravinesh A. Kumar, Kevin A. McGhee, Stephen Leach, Russell Bonaguro, Alan Maclean, Rosalia Aguirre-Hernandez, Brett S. Abrahams, Emil F. Coccaro, Sheilagh Hodgins, Gustavo Turecki, Anne Condon, Walter J. Muir, Angela R. Brooks-Wilson, Douglas H. Blackwood, Elizabeth M. Simpson

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Nuclear receptor 2E1 gene (NR2E1) resides within a 6q21-22 locus for bipolar disorder and schizophrenia. Mice deleted for Nr2e1 show altered neurogenesis, cortical and limbic abnormalities, aggression, hyperexcitability, and cognitive impairment. NR2E1 is therefore a positional and functional candidate for involvement in mental illness. We performed association analyses in 394 patients with bipolar disorder, 396 with schizophrenia, and 479 controls using six common markers and haplotypes. We also performed a comprehensive mutation screen of NR2E1, resequencing its entire coding region, complete 5′ and 3′ untranslated regions, consensus splice-sites, and evolutionarily conserved regions in 126 humans with bipolar disorder, schizophrenia, or aggressive disorders. NR2E1 was associated with bipolar disorder I and II [odds ratio (OR = 0.77, P = 0.013), bipolar disorder I (OR = 0.77, P = 0.015), bipolar disorder in females (OR = 0.72, P = 0.009), and with age at onset ≤25 years (OR = 0.67, P = 0.006)], all of which remained significant after correcting for multiple comparisons. We identified eight novel candidate mutations that were absent in 325 controls; four of these were predicted to alter known neural transcription factor binding sites. Analyses of NR2E1 mRNA in human brain revealed forebrain-specific transcription. The data presented support the hypothesis that genetic variation at NR2E1 may be associated with susceptibility to brain-behavior disorders.

Original languageEnglish (US)
Pages (from-to)880-889
Number of pages10
JournalAmerican Journal of Medical Genetics, Part B: Neuropsychiatric Genetics
Volume147
Issue number6
DOIs
StatePublished - Sep 5 2008
Externally publishedYes

Fingerprint

Cytoplasmic and Nuclear Receptors
Aggression
Bipolar Disorder
Schizophrenia
Mutation
Genes
5' Untranslated Regions
Neurogenesis
Brain Diseases
3' Untranslated Regions
Prosencephalon
Age of Onset
Mental Disorders
Haplotypes
Consensus
Transcription Factors
Odds Ratio
Binding Sites
Messenger RNA
Brain

Keywords

  • "Fierce" mice
  • Brain
  • Mental illness
  • Nuclear receptor
  • Polymorphisms

ASJC Scopus subject areas

  • Genetics(clinical)
  • Neuropsychology and Physiological Psychology
  • Neuroscience(all)

Cite this

Initial association of NR2E1 with bipolar disorder and identification of candidate mutations in bipolar disorder, schizophrenia, and aggression through resequencing. / Kumar, Ravinesh A.; McGhee, Kevin A.; Leach, Stephen; Bonaguro, Russell; Maclean, Alan; Aguirre-Hernandez, Rosalia; Abrahams, Brett S.; Coccaro, Emil F.; Hodgins, Sheilagh; Turecki, Gustavo; Condon, Anne; Muir, Walter J.; Brooks-Wilson, Angela R.; Blackwood, Douglas H.; Simpson, Elizabeth M.

In: American Journal of Medical Genetics, Part B: Neuropsychiatric Genetics, Vol. 147, No. 6, 05.09.2008, p. 880-889.

Research output: Contribution to journalArticle

Kumar, RA, McGhee, KA, Leach, S, Bonaguro, R, Maclean, A, Aguirre-Hernandez, R, Abrahams, BS, Coccaro, EF, Hodgins, S, Turecki, G, Condon, A, Muir, WJ, Brooks-Wilson, AR, Blackwood, DH & Simpson, EM 2008, 'Initial association of NR2E1 with bipolar disorder and identification of candidate mutations in bipolar disorder, schizophrenia, and aggression through resequencing', American Journal of Medical Genetics, Part B: Neuropsychiatric Genetics, vol. 147, no. 6, pp. 880-889. https://doi.org/10.1002/ajmg.b.30696
Kumar, Ravinesh A. ; McGhee, Kevin A. ; Leach, Stephen ; Bonaguro, Russell ; Maclean, Alan ; Aguirre-Hernandez, Rosalia ; Abrahams, Brett S. ; Coccaro, Emil F. ; Hodgins, Sheilagh ; Turecki, Gustavo ; Condon, Anne ; Muir, Walter J. ; Brooks-Wilson, Angela R. ; Blackwood, Douglas H. ; Simpson, Elizabeth M. / Initial association of NR2E1 with bipolar disorder and identification of candidate mutations in bipolar disorder, schizophrenia, and aggression through resequencing. In: American Journal of Medical Genetics, Part B: Neuropsychiatric Genetics. 2008 ; Vol. 147, No. 6. pp. 880-889.
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abstract = "Nuclear receptor 2E1 gene (NR2E1) resides within a 6q21-22 locus for bipolar disorder and schizophrenia. Mice deleted for Nr2e1 show altered neurogenesis, cortical and limbic abnormalities, aggression, hyperexcitability, and cognitive impairment. NR2E1 is therefore a positional and functional candidate for involvement in mental illness. We performed association analyses in 394 patients with bipolar disorder, 396 with schizophrenia, and 479 controls using six common markers and haplotypes. We also performed a comprehensive mutation screen of NR2E1, resequencing its entire coding region, complete 5′ and 3′ untranslated regions, consensus splice-sites, and evolutionarily conserved regions in 126 humans with bipolar disorder, schizophrenia, or aggressive disorders. NR2E1 was associated with bipolar disorder I and II [odds ratio (OR = 0.77, P = 0.013), bipolar disorder I (OR = 0.77, P = 0.015), bipolar disorder in females (OR = 0.72, P = 0.009), and with age at onset ≤25 years (OR = 0.67, P = 0.006)], all of which remained significant after correcting for multiple comparisons. We identified eight novel candidate mutations that were absent in 325 controls; four of these were predicted to alter known neural transcription factor binding sites. Analyses of NR2E1 mRNA in human brain revealed forebrain-specific transcription. The data presented support the hypothesis that genetic variation at NR2E1 may be associated with susceptibility to brain-behavior disorders.",
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AU - McGhee, Kevin A.

AU - Leach, Stephen

AU - Bonaguro, Russell

AU - Maclean, Alan

AU - Aguirre-Hernandez, Rosalia

AU - Abrahams, Brett S.

AU - Coccaro, Emil F.

AU - Hodgins, Sheilagh

AU - Turecki, Gustavo

AU - Condon, Anne

AU - Muir, Walter J.

AU - Brooks-Wilson, Angela R.

AU - Blackwood, Douglas H.

AU - Simpson, Elizabeth M.

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N2 - Nuclear receptor 2E1 gene (NR2E1) resides within a 6q21-22 locus for bipolar disorder and schizophrenia. Mice deleted for Nr2e1 show altered neurogenesis, cortical and limbic abnormalities, aggression, hyperexcitability, and cognitive impairment. NR2E1 is therefore a positional and functional candidate for involvement in mental illness. We performed association analyses in 394 patients with bipolar disorder, 396 with schizophrenia, and 479 controls using six common markers and haplotypes. We also performed a comprehensive mutation screen of NR2E1, resequencing its entire coding region, complete 5′ and 3′ untranslated regions, consensus splice-sites, and evolutionarily conserved regions in 126 humans with bipolar disorder, schizophrenia, or aggressive disorders. NR2E1 was associated with bipolar disorder I and II [odds ratio (OR = 0.77, P = 0.013), bipolar disorder I (OR = 0.77, P = 0.015), bipolar disorder in females (OR = 0.72, P = 0.009), and with age at onset ≤25 years (OR = 0.67, P = 0.006)], all of which remained significant after correcting for multiple comparisons. We identified eight novel candidate mutations that were absent in 325 controls; four of these were predicted to alter known neural transcription factor binding sites. Analyses of NR2E1 mRNA in human brain revealed forebrain-specific transcription. The data presented support the hypothesis that genetic variation at NR2E1 may be associated with susceptibility to brain-behavior disorders.

AB - Nuclear receptor 2E1 gene (NR2E1) resides within a 6q21-22 locus for bipolar disorder and schizophrenia. Mice deleted for Nr2e1 show altered neurogenesis, cortical and limbic abnormalities, aggression, hyperexcitability, and cognitive impairment. NR2E1 is therefore a positional and functional candidate for involvement in mental illness. We performed association analyses in 394 patients with bipolar disorder, 396 with schizophrenia, and 479 controls using six common markers and haplotypes. We also performed a comprehensive mutation screen of NR2E1, resequencing its entire coding region, complete 5′ and 3′ untranslated regions, consensus splice-sites, and evolutionarily conserved regions in 126 humans with bipolar disorder, schizophrenia, or aggressive disorders. NR2E1 was associated with bipolar disorder I and II [odds ratio (OR = 0.77, P = 0.013), bipolar disorder I (OR = 0.77, P = 0.015), bipolar disorder in females (OR = 0.72, P = 0.009), and with age at onset ≤25 years (OR = 0.67, P = 0.006)], all of which remained significant after correcting for multiple comparisons. We identified eight novel candidate mutations that were absent in 325 controls; four of these were predicted to alter known neural transcription factor binding sites. Analyses of NR2E1 mRNA in human brain revealed forebrain-specific transcription. The data presented support the hypothesis that genetic variation at NR2E1 may be associated with susceptibility to brain-behavior disorders.

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KW - Mental illness

KW - Nuclear receptor

KW - Polymorphisms

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