Inhibitory effects of human amniotic mesenchyme stem cells on C6 glioma cells in tumor-bearing mice

Yuan Li, Hong Liang Jiao, Fang Xia Guan, Lai Jun Song, Bo Yang, Xiang Hu, Ying Du, Hui Xiang Li, Xia Pang, Fu Sheng Liu, Jing Li Xue

Research output: Contribution to journalArticlepeer-review

Abstract

Background: Umbilical cord blood or amniotic mesenchymal stem cells are characterized by affinity tumor and inhibiting tumor. Objective: To explore inhibitory effects of the human amniotic mesenchyme stem cells on C6 glioma cells. Design, time and setting: The cytological in vivo controlled experiment was performed at the Department of Microbiology and Immunology, Zhengzhou University from June to September 2008. Materials: The amnion membrane was harvested from healthy puerperants under strict sterile conditions at the First Affiliated Hospital, Zhengzhou University. C6 glioma cells were presented by Beijing Neurosurgery Institute. Ten BALR/c nude mice were bought from Shanghai Silaike Experimental Animal Co., Ltd. Methods: Placenta was sterily obtained to separate some amniotic membrane. Human amniotic mesenchymal stem cells were harvested in vitro. At the third passage, cells were used for this study. DMEM/F-12 culture-medium with 3×106 C6 glioma cells was infused into two sides of subcutaneous back of ten nude mice to induce models of mice with tumor. Following model establishment, 50 μ L of DMEM/F-12 was inoculated into the mouse left side of subcutaneous tissues in the model control group. An equal volume of DMEM/F-12 containing 2×106 amniotic mesenchymal stem cells was injected into the right side of subcutaneous tissues in the cell transplantation group. Main outcome measures: Growth of the glioma, infiltration of surrounding tissues and neovascularization were measured. Cyclin D1 expression in tumor was detected using immumohistochemistry. Results: The tumor volume was significantly smaller in the cell transplantation group than in the model control group at days 14 and 21 following cell transplantation (F=54.127, P < 0.05). In the model control group, the tumor was nodular growth, necrotic in the center, with the presence of neovascularization and obvious infiltration in the muscle and skin, even in the peritoneum. In the cell transplantation group, few sublobe slightly skin infiltration were found, but no neovascularization or muscular layer infiltration. Cyclin D1 protein was positively expressed in the model control group, whereas expressed with low positive rate in the cell transplantation group. Conclusion: Human amniotic mesenchymal stem cells can dramatically inhibit the growth of C6 glioma cells and the expression of Cyclin D1.

Original languageEnglish (US)
Pages (from-to)10099-10103
Number of pages5
JournalJournal of Clinical Rehabilitative Tissue Engineering Research
Volume12
Issue number51
StatePublished - Dec 16 2008

ASJC Scopus subject areas

  • Orthopedics and Sports Medicine
  • Biomedical Engineering
  • Clinical Biochemistry

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