Inhibition of Serratia marcescens nuclease secretion by a truncated nuclease peptide

Yi Chi Chen, Yousin Suh, Erik Riise, Bo Kartman, Shida Jin, Michael J. Benedik

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The production of extracellular nuclease (Nuc) from the Serratia marcescens nucA chromosomal locus is inhibited in cells producing the N-terminal portion of Nuc from a multicopy plasmid. This inhibition in trans is not at the level of nucA expression, but rather at the level of secretion of the Nuc protein. Production of the periplasmic protein β-lactamase (B1a) does not inhibit Nuc production unless fused to the nucA signal peptide and expressed from nucAp. Inhibition by either the truncated Nuc peptide (ΔNuc) or a B1a fusion protein is promoter specific and observed when expressed from nucAp; little inhibition is observed when the same protein is expressed from the lacZpo promoter-operator. This promoter specificity is also true for the secretion of Nuc itself.

Original languageEnglish (US)
Pages (from-to)9-16
Number of pages8
JournalGene
Volume172
Issue number1
DOIs
StatePublished - Jun 12 1996
Externally publishedYes

Fingerprint

Serratia marcescens nuclease
Peptides
Periplasmic Proteins
Proteins
Protein Sorting Signals
Plasmids

Keywords

  • Dominant mutation
  • Extracellular proteins
  • Fusion protein
  • Gene deletion

ASJC Scopus subject areas

  • Genetics

Cite this

Inhibition of Serratia marcescens nuclease secretion by a truncated nuclease peptide. / Chen, Yi Chi; Suh, Yousin; Riise, Erik; Kartman, Bo; Jin, Shida; Benedik, Michael J.

In: Gene, Vol. 172, No. 1, 12.06.1996, p. 9-16.

Research output: Contribution to journalArticle

Chen, Yi Chi ; Suh, Yousin ; Riise, Erik ; Kartman, Bo ; Jin, Shida ; Benedik, Michael J. / Inhibition of Serratia marcescens nuclease secretion by a truncated nuclease peptide. In: Gene. 1996 ; Vol. 172, No. 1. pp. 9-16.
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AU - Benedik, Michael J.

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