Inhibition of Serratia marcescens nuclease secretion by a truncated nuclease peptide

Yi Chi Chen; Yousin Suh; Erik Riise; Bo Kartman; Shida Jin; Michael J. Benedik

doi:10.1016/0378-1119(96)00187-4

Inhibition of Serratia marcescens nuclease secretion by a truncated nuclease peptide

Yi Chi Chen, Yousin Suh, Erik Riise, Bo Kartman, Shida Jin, Michael J. Benedik

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

The production of extracellular nuclease (Nuc) from the Serratia marcescens nucA chromosomal locus is inhibited in cells producing the N-terminal portion of Nuc from a multicopy plasmid. This inhibition in trans is not at the level of nucA expression, but rather at the level of secretion of the Nuc protein. Production of the periplasmic protein β-lactamase (B1a) does not inhibit Nuc production unless fused to the nucA signal peptide and expressed from nucAp. Inhibition by either the truncated Nuc peptide (ΔNuc) or a B1a fusion protein is promoter specific and observed when expressed from nucAp; little inhibition is observed when the same protein is expressed from the lacZpo promoter-operator. This promoter specificity is also true for the secretion of Nuc itself.

Original language	English (US)
Pages (from-to)	9-16
Number of pages	8
Journal	Gene
Volume	172
Issue number	1
DOIs	https://doi.org/10.1016/0378-1119(96)00187-4
State	Published - Jun 12 1996
Externally published	Yes

Keywords

Dominant mutation
Extracellular proteins
Fusion protein
Gene deletion

ASJC Scopus subject areas

Genetics

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10.1016/0378-1119(96)00187-4

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title = "Inhibition of Serratia marcescens nuclease secretion by a truncated nuclease peptide",

abstract = "The production of extracellular nuclease (Nuc) from the Serratia marcescens nucA chromosomal locus is inhibited in cells producing the N-terminal portion of Nuc from a multicopy plasmid. This inhibition in trans is not at the level of nucA expression, but rather at the level of secretion of the Nuc protein. Production of the periplasmic protein β-lactamase (B1a) does not inhibit Nuc production unless fused to the nucA signal peptide and expressed from nucAp. Inhibition by either the truncated Nuc peptide (ΔNuc) or a B1a fusion protein is promoter specific and observed when expressed from nucAp; little inhibition is observed when the same protein is expressed from the lacZpo promoter-operator. This promoter specificity is also true for the secretion of Nuc itself.",

keywords = "Dominant mutation, Extracellular proteins, Fusion protein, Gene deletion",

author = "Chen, {Yi Chi} and Yousin Suh and Erik Riise and Bo Kartman and Shida Jin and Benedik, {Michael J.}",

note = "Funding Information: This work was supported by NIH grant GM36891 and #36521178 from the Texas Advanced Research Program to MJB. National Science Foundation grant DIR 9109294 provided some equipment used in this work. We would like to thank Soren Molin and Michael Givskov for helpful discussions and hospitality.",

year = "1996",

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doi = "10.1016/0378-1119(96)00187-4",

language = "English (US)",

volume = "172",

pages = "9--16",

journal = "Gene",

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T1 - Inhibition of Serratia marcescens nuclease secretion by a truncated nuclease peptide

AU - Chen, Yi Chi

AU - Suh, Yousin

AU - Riise, Erik

AU - Kartman, Bo

AU - Jin, Shida

AU - Benedik, Michael J.

N1 - Funding Information: This work was supported by NIH grant GM36891 and #36521178 from the Texas Advanced Research Program to MJB. National Science Foundation grant DIR 9109294 provided some equipment used in this work. We would like to thank Soren Molin and Michael Givskov for helpful discussions and hospitality.

PY - 1996/6/12

Y1 - 1996/6/12

N2 - The production of extracellular nuclease (Nuc) from the Serratia marcescens nucA chromosomal locus is inhibited in cells producing the N-terminal portion of Nuc from a multicopy plasmid. This inhibition in trans is not at the level of nucA expression, but rather at the level of secretion of the Nuc protein. Production of the periplasmic protein β-lactamase (B1a) does not inhibit Nuc production unless fused to the nucA signal peptide and expressed from nucAp. Inhibition by either the truncated Nuc peptide (ΔNuc) or a B1a fusion protein is promoter specific and observed when expressed from nucAp; little inhibition is observed when the same protein is expressed from the lacZpo promoter-operator. This promoter specificity is also true for the secretion of Nuc itself.

AB - The production of extracellular nuclease (Nuc) from the Serratia marcescens nucA chromosomal locus is inhibited in cells producing the N-terminal portion of Nuc from a multicopy plasmid. This inhibition in trans is not at the level of nucA expression, but rather at the level of secretion of the Nuc protein. Production of the periplasmic protein β-lactamase (B1a) does not inhibit Nuc production unless fused to the nucA signal peptide and expressed from nucAp. Inhibition by either the truncated Nuc peptide (ΔNuc) or a B1a fusion protein is promoter specific and observed when expressed from nucAp; little inhibition is observed when the same protein is expressed from the lacZpo promoter-operator. This promoter specificity is also true for the secretion of Nuc itself.

KW - Dominant mutation

KW - Extracellular proteins

KW - Fusion protein

KW - Gene deletion

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SN - 0378-1119

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JO - Gene

JF - Gene

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Inhibition of Serratia marcescens nuclease secretion by a truncated nuclease peptide

Abstract

Keywords

ASJC Scopus subject areas

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