Inhibition of proliferation and stimulation of migration of endothelial cells by secretoneurin in vitro

Christian M. Kähler, Rudolf Kirchmair, Gerhard Kaufmann, Stefan T. Kähler, Norbert Reinisch, Reiner Fischer-Colbrie, Ruth Hogue-Angeletti, Hans Winkler, Christian J. Wiedermann

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

Vascular cell responses in inflammation are affected by several neuropeptides of perivascular nerve fibers. Secretoneurin is a 33-amino acid peptide that is coreleased from these nerve endings with other proinflammatory neuropeptides, eg, substance P and calcitonin gene-related peptide. Furthermore, secretoneurin has been shown to be chemotactic for human skin fibroblasts and human blood monocytes in vitro and in vivo. An action on cellular components of the vascular wall is not yet reported. We therefore investigated in vitro effects of this novel sensory neuropeptide on endothelial cells. Secretoneurin exerted a potent and reversible inhibitory effect both on endothelial cell growth under low serum conditions (1% fetal calf serum) and endothelial cell growth factor-activated endothelial cell proliferation. We show in the present study that secretoneurin exerts this effect on aortic (rat) and pulmonary artery (bovine) endothelial cells, as well as venous (human umbilical vein) endothelium. Endothelial cell chemotaxis was tested by means of three different migration assays employing nitrocellulose and polycarbonate micropore filters. Secretoneurin consistently exhibited potent chemoattractant activity. The effective concentrations for the observed effects were in the picomolar range. The combination of chemotactic and antiproliferative effects on endothelial cells suggests that secretoneurin may act as a regulatory factor of vascular cell functions.

Original languageEnglish (US)
Pages (from-to)932-939
Number of pages8
JournalArteriosclerosis, Thrombosis, and Vascular Biology
Volume17
Issue number5
StatePublished - 1997

Fingerprint

Endothelial Cells
Neuropeptides
polycarbonate
Blood Vessels
Endothelial Growth Factors
Micropore Filters
Umbilical Veins
Collodion
Nerve Endings
Calcitonin Gene-Related Peptide
Chemotactic Factors
Chemotaxis
Substance P
In Vitro Techniques
secretoneurin
Inhibition (Psychology)
Serum
Nerve Fibers
Pulmonary Artery
Endothelium

Keywords

  • endothelial cells
  • migration
  • neuropeptide
  • proliferation
  • secretoneurin

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

Cite this

Kähler, C. M., Kirchmair, R., Kaufmann, G., Kähler, S. T., Reinisch, N., Fischer-Colbrie, R., ... Wiedermann, C. J. (1997). Inhibition of proliferation and stimulation of migration of endothelial cells by secretoneurin in vitro. Arteriosclerosis, Thrombosis, and Vascular Biology, 17(5), 932-939.

Inhibition of proliferation and stimulation of migration of endothelial cells by secretoneurin in vitro. / Kähler, Christian M.; Kirchmair, Rudolf; Kaufmann, Gerhard; Kähler, Stefan T.; Reinisch, Norbert; Fischer-Colbrie, Reiner; Hogue-Angeletti, Ruth; Winkler, Hans; Wiedermann, Christian J.

In: Arteriosclerosis, Thrombosis, and Vascular Biology, Vol. 17, No. 5, 1997, p. 932-939.

Research output: Contribution to journalArticle

Kähler, CM, Kirchmair, R, Kaufmann, G, Kähler, ST, Reinisch, N, Fischer-Colbrie, R, Hogue-Angeletti, R, Winkler, H & Wiedermann, CJ 1997, 'Inhibition of proliferation and stimulation of migration of endothelial cells by secretoneurin in vitro', Arteriosclerosis, Thrombosis, and Vascular Biology, vol. 17, no. 5, pp. 932-939.
Kähler CM, Kirchmair R, Kaufmann G, Kähler ST, Reinisch N, Fischer-Colbrie R et al. Inhibition of proliferation and stimulation of migration of endothelial cells by secretoneurin in vitro. Arteriosclerosis, Thrombosis, and Vascular Biology. 1997;17(5):932-939.
Kähler, Christian M. ; Kirchmair, Rudolf ; Kaufmann, Gerhard ; Kähler, Stefan T. ; Reinisch, Norbert ; Fischer-Colbrie, Reiner ; Hogue-Angeletti, Ruth ; Winkler, Hans ; Wiedermann, Christian J. / Inhibition of proliferation and stimulation of migration of endothelial cells by secretoneurin in vitro. In: Arteriosclerosis, Thrombosis, and Vascular Biology. 1997 ; Vol. 17, No. 5. pp. 932-939.
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