Inhibition of interferon γ signaling by the short chain fatty acid butyrate

Lidija Klampfer, Jie Huang, Takehiko Sasazuki, Senji Shirasawa, Leonard H. Augenlicht

Research output: Contribution to journalArticle

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Abstract

The short chain fatty acid butyrate promotes proliferation and survival of normal epithelial cells, but induces G1 or G2-M arrest in transformed cells, which is coupled to differentiation and apoptosis. Local administration of butyrate has been shown to ameliorate inflammation in ulcerative colitis; however, the precise mechanism of its anti-inflammatory activity is not known. IFN-γ is one of the principle cytokines secreted by lamina propria cells in inflamed mucosa and elevated levels of the transcription factor required for IFN-γ signaling, STAT1 (signal transducer and activator of transcription 1), are present in the colonic mucosa of patients with ulcerative colitis and Crohn's disease. Here we report that butyrate is a strong inhibitor of signaling by IFN-γ. We demonstrated that this short chain fatty acid inhibits IFN-γ-induced tyrosine and serine phosphorylation of STAT1. IFN-γ-induced JAK2 activation was inhibited by butyrate, implicating JAK2 as a target of butyrate action. Accordingly, STAT1 nuclear translocation and its DNA binding were completely inhibited in butyrate-treated cells. Transient transfection experiments using a reporter gene construct containing eight GAS sites (γ-activated sites) revealed that butyrate inhibits IFN-γ induced, STAT1-dependent, transcriptional activation. Proinflammatory cytokines, including IFN-γ, play an important role in the pathogenesis of inflammatory bowel disease, and abnormal activity of STAT1 is associated with human malignancies and intestinal inflammatory diseases. Thus, our data suggest that butyrate negatively regulates mucosal inflammation through the inhibition of IFN-γ/STAT1 signaling.

Original languageEnglish (US)
Pages (from-to)855-862
Number of pages8
JournalMolecular Cancer Research
Volume1
Issue number11
StatePublished - Sep 1 2003

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Volatile Fatty Acids
Butyrates
STAT1 Transcription Factor
Interferons
Mucous Membrane
Ulcerative Colitis
Cytokines
Inflammation
Intestinal Diseases
Reporter Genes
Inflammatory Bowel Diseases
Crohn Disease
Serine
Transcriptional Activation
Transfection
Tyrosine
Anti-Inflammatory Agents
Transcription Factors
Epithelial Cells
Phosphorylation

ASJC Scopus subject areas

  • Molecular Biology
  • Cancer Research

Cite this

Inhibition of interferon γ signaling by the short chain fatty acid butyrate. / Klampfer, Lidija; Huang, Jie; Sasazuki, Takehiko; Shirasawa, Senji; Augenlicht, Leonard H.

In: Molecular Cancer Research, Vol. 1, No. 11, 01.09.2003, p. 855-862.

Research output: Contribution to journalArticle

Klampfer, L, Huang, J, Sasazuki, T, Shirasawa, S & Augenlicht, LH 2003, 'Inhibition of interferon γ signaling by the short chain fatty acid butyrate', Molecular Cancer Research, vol. 1, no. 11, pp. 855-862.
Klampfer, Lidija ; Huang, Jie ; Sasazuki, Takehiko ; Shirasawa, Senji ; Augenlicht, Leonard H. / Inhibition of interferon γ signaling by the short chain fatty acid butyrate. In: Molecular Cancer Research. 2003 ; Vol. 1, No. 11. pp. 855-862.
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