Inhibition of development of characteristic indices of myeloid differentiation by a factor released from a tetraploid variant of the human promyelocytic cell line, HL-60

Naomi Mendelsohn, Tina M. Calderon, George Acs, Judith K. Christman

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

HL-60TR, a tetraploid variant of the human promyeloid cell line HL-60, was obtained by culturing HL-60 cells for one week with the tumor promoter 12-O-tetradecanoylphorbol-13-acetate (TPA) at 400 pM. HL-60TR cells have lost the ability to develop several common markers of maturation in response to compounds that induce monocytoid or myelocytoid differentiation of HL-60 cells. In addition, they release a factor which inhibits induction of the same markers in HL-60 cells. Medium conditioned by HL-60TR cells also inhibits colony formation by normal mouse bone marrow cells. These properties have been maintained by HL-60TR cells through more than one year of constant subculture in the absence of TPA, a finding which suggests the possibility that TPA may promote tumor formation not only through direct effects on the phenotype of initiated cells but also through induction of continued production of factors that affect differentiation of normal stem cells.

Original languageEnglish (US)
Pages (from-to)514-519
Number of pages6
JournalExperimental Cell Research
Volume148
Issue number2
DOIs
StatePublished - Oct 15 1983
Externally publishedYes

Fingerprint

Tetraploidy
HL-60 Cells
Tetradecanoylphorbol Acetate
Cell Line
Conditioned Culture Medium
Bone Marrow Cells
Carcinogens
Stem Cells
Phenotype
Neoplasms

ASJC Scopus subject areas

  • Cell Biology

Cite this

Inhibition of development of characteristic indices of myeloid differentiation by a factor released from a tetraploid variant of the human promyelocytic cell line, HL-60. / Mendelsohn, Naomi; Calderon, Tina M.; Acs, George; Christman, Judith K.

In: Experimental Cell Research, Vol. 148, No. 2, 15.10.1983, p. 514-519.

Research output: Contribution to journalArticle

@article{00e298d1cd7a43178c32dd6c2947f852,
title = "Inhibition of development of characteristic indices of myeloid differentiation by a factor released from a tetraploid variant of the human promyelocytic cell line, HL-60",
abstract = "HL-60TR, a tetraploid variant of the human promyeloid cell line HL-60, was obtained by culturing HL-60 cells for one week with the tumor promoter 12-O-tetradecanoylphorbol-13-acetate (TPA) at 400 pM. HL-60TR cells have lost the ability to develop several common markers of maturation in response to compounds that induce monocytoid or myelocytoid differentiation of HL-60 cells. In addition, they release a factor which inhibits induction of the same markers in HL-60 cells. Medium conditioned by HL-60TR cells also inhibits colony formation by normal mouse bone marrow cells. These properties have been maintained by HL-60TR cells through more than one year of constant subculture in the absence of TPA, a finding which suggests the possibility that TPA may promote tumor formation not only through direct effects on the phenotype of initiated cells but also through induction of continued production of factors that affect differentiation of normal stem cells.",
author = "Naomi Mendelsohn and Calderon, {Tina M.} and George Acs and Christman, {Judith K.}",
year = "1983",
month = "10",
day = "15",
doi = "10.1016/0014-4827(83)90173-8",
language = "English (US)",
volume = "148",
pages = "514--519",
journal = "Experimental Cell Research",
issn = "0014-4827",
publisher = "Academic Press Inc.",
number = "2",

}

TY - JOUR

T1 - Inhibition of development of characteristic indices of myeloid differentiation by a factor released from a tetraploid variant of the human promyelocytic cell line, HL-60

AU - Mendelsohn, Naomi

AU - Calderon, Tina M.

AU - Acs, George

AU - Christman, Judith K.

PY - 1983/10/15

Y1 - 1983/10/15

N2 - HL-60TR, a tetraploid variant of the human promyeloid cell line HL-60, was obtained by culturing HL-60 cells for one week with the tumor promoter 12-O-tetradecanoylphorbol-13-acetate (TPA) at 400 pM. HL-60TR cells have lost the ability to develop several common markers of maturation in response to compounds that induce monocytoid or myelocytoid differentiation of HL-60 cells. In addition, they release a factor which inhibits induction of the same markers in HL-60 cells. Medium conditioned by HL-60TR cells also inhibits colony formation by normal mouse bone marrow cells. These properties have been maintained by HL-60TR cells through more than one year of constant subculture in the absence of TPA, a finding which suggests the possibility that TPA may promote tumor formation not only through direct effects on the phenotype of initiated cells but also through induction of continued production of factors that affect differentiation of normal stem cells.

AB - HL-60TR, a tetraploid variant of the human promyeloid cell line HL-60, was obtained by culturing HL-60 cells for one week with the tumor promoter 12-O-tetradecanoylphorbol-13-acetate (TPA) at 400 pM. HL-60TR cells have lost the ability to develop several common markers of maturation in response to compounds that induce monocytoid or myelocytoid differentiation of HL-60 cells. In addition, they release a factor which inhibits induction of the same markers in HL-60 cells. Medium conditioned by HL-60TR cells also inhibits colony formation by normal mouse bone marrow cells. These properties have been maintained by HL-60TR cells through more than one year of constant subculture in the absence of TPA, a finding which suggests the possibility that TPA may promote tumor formation not only through direct effects on the phenotype of initiated cells but also through induction of continued production of factors that affect differentiation of normal stem cells.

UR - http://www.scopus.com/inward/record.url?scp=0021052248&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0021052248&partnerID=8YFLogxK

U2 - 10.1016/0014-4827(83)90173-8

DO - 10.1016/0014-4827(83)90173-8

M3 - Article

C2 - 6605257

AN - SCOPUS:0021052248

VL - 148

SP - 514

EP - 519

JO - Experimental Cell Research

JF - Experimental Cell Research

SN - 0014-4827

IS - 2

ER -