Inhibition of deoxyhemoglobin S polymerization by glyceraldehyde

A. Seetharama Acharya, Leslie G. Sussman, Wanda M. Jones, James M. Manning

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Glyceraldehyde reacts with hemoglobin S in the intact erythrocyte to reduce the degree of polymerization, thereby inhibiting sickling of the erythrocyte. Only five of the 24 amino groups per αβ dimer react with glyceraldehyde; the adducts are present as ketoamine structures, formed by Amadori rearrangement of the initial Schiff base adducts on the protein. The reactive amino groups are the ε{lunate}-amino group of Lys-16 of the α-chain, and the α-amino group of Val-1 as well as the ε{lunate}-amino groups Lys-82, Lys-59, and Lys-120 of the β-chain. Hybrid tetramers were prepared with the modification only on Lys-16 of the α-chain or on the reactive lysine residues of the β-chain. The former derivative gels at a much higher hemoglobin concentration (23 g/dl) than either the latter derivative (16 g/dl) or unmodified deoxyhemoglobin S (15 g/dl). Thus, the modification at Lys-16 of the α-chain is a major factor in the inhibition of sickling by glyceraldehyde.

Original languageEnglish (US)
Pages (from-to)101-109
Number of pages9
JournalAnalytical Biochemistry
Volume136
Issue number1
DOIs
StatePublished - 1984
Externally publishedYes

Fingerprint

Glyceraldehyde
Sickle Hemoglobin
Polymerization
Erythrocytes
Derivatives
Schiff Bases
Dimers
Lysine
Hemoglobins
Gels
Proteins

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Acharya, A. S., Sussman, L. G., Jones, W. M., & Manning, J. M. (1984). Inhibition of deoxyhemoglobin S polymerization by glyceraldehyde. Analytical Biochemistry, 136(1), 101-109. https://doi.org/10.1016/0003-2697(84)90311-7

Inhibition of deoxyhemoglobin S polymerization by glyceraldehyde. / Acharya, A. Seetharama; Sussman, Leslie G.; Jones, Wanda M.; Manning, James M.

In: Analytical Biochemistry, Vol. 136, No. 1, 1984, p. 101-109.

Research output: Contribution to journalArticle

Acharya, AS, Sussman, LG, Jones, WM & Manning, JM 1984, 'Inhibition of deoxyhemoglobin S polymerization by glyceraldehyde', Analytical Biochemistry, vol. 136, no. 1, pp. 101-109. https://doi.org/10.1016/0003-2697(84)90311-7
Acharya, A. Seetharama ; Sussman, Leslie G. ; Jones, Wanda M. ; Manning, James M. / Inhibition of deoxyhemoglobin S polymerization by glyceraldehyde. In: Analytical Biochemistry. 1984 ; Vol. 136, No. 1. pp. 101-109.
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