Abstract
By use of whole cell patch‐clamp and Indo‐1 fluorescence studies of the Jurkat T leukaemic cell line, we show that the new organic antagonist of receptor‐mediated Ca2+ entry, SK&F 96365, inhibits the T cell Ca2+ current in a dose‐dependent fashion, with an IC50 of 12 μm. SK&F 96365 also inhibits [3H]‐thymidine incorporation and interleukin‐2 (IL‐2) synthesis in peripheral blood lymphocytes. SK&F 96365 has no effect on Ca2+ stores release or K+ channels. This is the first account of an organic inhibitor of the T cell Ca2+ current. The ability of SK&F 96365 to inhibit IL‐2 synthesis and cell proliferation suggests that a new class of related Ca2+ channel blockers can be developed as immunosuppressive agents. 1994 British Pharmacological Society
Original language | English (US) |
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Pages (from-to) | 861-868 |
Number of pages | 8 |
Journal | British Journal of Pharmacology |
Volume | 113 |
Issue number | 3 |
DOIs | |
State | Published - Nov 1994 |
Externally published | Yes |
Keywords
- Ca channels
- SK&F 96365
- T lymphocytes
ASJC Scopus subject areas
- Pharmacology