Inhibition by SK&F 96365 of Ca2+ current, IL‐2 production and activation in T lymphocytes

S. Clare Chung, Thomas V. McDonald, Phyllis Gardner

Research output: Contribution to journalArticle

90 Scopus citations

Abstract

By use of whole cell patch‐clamp and Indo‐1 fluorescence studies of the Jurkat T leukaemic cell line, we show that the new organic antagonist of receptor‐mediated Ca2+ entry, SK&F 96365, inhibits the T cell Ca2+ current in a dose‐dependent fashion, with an IC50 of 12 μm. SK&F 96365 also inhibits [3H]‐thymidine incorporation and interleukin‐2 (IL‐2) synthesis in peripheral blood lymphocytes. SK&F 96365 has no effect on Ca2+ stores release or K+ channels. This is the first account of an organic inhibitor of the T cell Ca2+ current. The ability of SK&F 96365 to inhibit IL‐2 synthesis and cell proliferation suggests that a new class of related Ca2+ channel blockers can be developed as immunosuppressive agents. 1994 British Pharmacological Society

Original languageEnglish (US)
Pages (from-to)861-868
Number of pages8
JournalBritish Journal of Pharmacology
Volume113
Issue number3
DOIs
StatePublished - Nov 1994

Keywords

  • Ca channels
  • SK&F 96365
  • T lymphocytes

ASJC Scopus subject areas

  • Pharmacology

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