Inhibition by SK and F 96365 of Ca2+ current, IL-2 production and activation in T lymphocytes

S. C. Chung, T. V. McDonald, P. Gardner

Research output: Contribution to journalArticle

89 Citations (Scopus)

Abstract

1. By use of whole cell patch-c]amp and Indo-1 fluorescence studies of the Jurkat T leukaemic cell line, we show that the new organic antagonist of receptor-mediated Ca2+ entry, SK and F 96365, inhibits the T cell Ca2+ current in a dose-dependent fashion, with an IC50 of 12 μM. 2. SK and F 96365 also inhibits [3H]-thymidine incorporation and interleukin-2 (IL-2) synthesis in peripheral blood lymphocytes. 3. SK and F 96365 has no effect on Ca2+ stores release or K+ channels. 4. This is the first account of an organic inhibitor of the T cell Ca2+ current. The ability of SK and F 96365 to inhibit IL-2 synthesis and cell proliferation suggests that a new class of related Ca2+ channel blockers can be developed as immunosuppressive agents.

Original languageEnglish (US)
Pages (from-to)861-868
Number of pages8
JournalBritish Journal of Pharmacology
Volume113
Issue number3
StatePublished - 1994
Externally publishedYes

Fingerprint

1-(2-(3-(4-methoxyphenyl)propoxy)-4-methoxyphenylethyl)-1H-imidazole
Interleukin-2
T-Lymphocytes
Immunosuppressive Agents
Thymidine
Inhibitory Concentration 50
Fluorescence
Cell Proliferation
Lymphocytes
Cell Line

Keywords

  • Ca channels
  • SK and F 96365
  • T lymphocytes

ASJC Scopus subject areas

  • Pharmacology

Cite this

Inhibition by SK and F 96365 of Ca2+ current, IL-2 production and activation in T lymphocytes. / Chung, S. C.; McDonald, T. V.; Gardner, P.

In: British Journal of Pharmacology, Vol. 113, No. 3, 1994, p. 861-868.

Research output: Contribution to journalArticle

Chung, S. C. ; McDonald, T. V. ; Gardner, P. / Inhibition by SK and F 96365 of Ca2+ current, IL-2 production and activation in T lymphocytes. In: British Journal of Pharmacology. 1994 ; Vol. 113, No. 3. pp. 861-868.
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