Influence of trifluoroacetic acid on retention times of histidine-containing tryptic peptides in reverse phase HPLC.

A. S. Acharya, A. Di Donato, B. N. Manjula, V. A. Fischetti, J. M. Manning

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

The tryptic peptides of the aminoethylated alpha- and beta-chain of hemoglobin have been separated on a Partisil-10 ODS-2 column with a linear gradient of acetonitrile containing 0.1% trifluoroacetic acid. The elution profile of the tryptic peptides of the chains obtained with this acetonitrile trifluoroacetate system has been compared with that obtained using phosphoric acid as the ion-pairing reagent. This comparison demonstrated that trifluoroacetate influences the retention times of the histidine-containing tryptic peptides much more than it affects those peptides that do not contain histidine residues. This behavior has been rationalized on the basis of ion-pairing of trifluoroacetate with the histidine residues of the tryptic peptides.

Original languageEnglish (US)
Pages (from-to)78-82
Number of pages5
JournalInternational Journal of Peptide and Protein Research
Volume22
Issue number1
StatePublished - Jul 1983
Externally publishedYes

Fingerprint

Trifluoroacetic Acid
Histidine
High Pressure Liquid Chromatography
Peptides
Ions
Hemoglobins

ASJC Scopus subject areas

  • Biochemistry

Cite this

Influence of trifluoroacetic acid on retention times of histidine-containing tryptic peptides in reverse phase HPLC. / Acharya, A. S.; Di Donato, A.; Manjula, B. N.; Fischetti, V. A.; Manning, J. M.

In: International Journal of Peptide and Protein Research, Vol. 22, No. 1, 07.1983, p. 78-82.

Research output: Contribution to journalArticle

Acharya, A. S. ; Di Donato, A. ; Manjula, B. N. ; Fischetti, V. A. ; Manning, J. M. / Influence of trifluoroacetic acid on retention times of histidine-containing tryptic peptides in reverse phase HPLC. In: International Journal of Peptide and Protein Research. 1983 ; Vol. 22, No. 1. pp. 78-82.
@article{0004c31314a7490db877e4ce7b07d177,
title = "Influence of trifluoroacetic acid on retention times of histidine-containing tryptic peptides in reverse phase HPLC.",
abstract = "The tryptic peptides of the aminoethylated alpha- and beta-chain of hemoglobin have been separated on a Partisil-10 ODS-2 column with a linear gradient of acetonitrile containing 0.1{\%} trifluoroacetic acid. The elution profile of the tryptic peptides of the chains obtained with this acetonitrile trifluoroacetate system has been compared with that obtained using phosphoric acid as the ion-pairing reagent. This comparison demonstrated that trifluoroacetate influences the retention times of the histidine-containing tryptic peptides much more than it affects those peptides that do not contain histidine residues. This behavior has been rationalized on the basis of ion-pairing of trifluoroacetate with the histidine residues of the tryptic peptides.",
author = "Acharya, {A. S.} and {Di Donato}, A. and Manjula, {B. N.} and Fischetti, {V. A.} and Manning, {J. M.}",
year = "1983",
month = "7",
language = "English (US)",
volume = "22",
pages = "78--82",
journal = "International Journal of Peptide and Protein Research",
issn = "0367-8377",
publisher = "Wiley-Blackwell",
number = "1",

}

TY - JOUR

T1 - Influence of trifluoroacetic acid on retention times of histidine-containing tryptic peptides in reverse phase HPLC.

AU - Acharya, A. S.

AU - Di Donato, A.

AU - Manjula, B. N.

AU - Fischetti, V. A.

AU - Manning, J. M.

PY - 1983/7

Y1 - 1983/7

N2 - The tryptic peptides of the aminoethylated alpha- and beta-chain of hemoglobin have been separated on a Partisil-10 ODS-2 column with a linear gradient of acetonitrile containing 0.1% trifluoroacetic acid. The elution profile of the tryptic peptides of the chains obtained with this acetonitrile trifluoroacetate system has been compared with that obtained using phosphoric acid as the ion-pairing reagent. This comparison demonstrated that trifluoroacetate influences the retention times of the histidine-containing tryptic peptides much more than it affects those peptides that do not contain histidine residues. This behavior has been rationalized on the basis of ion-pairing of trifluoroacetate with the histidine residues of the tryptic peptides.

AB - The tryptic peptides of the aminoethylated alpha- and beta-chain of hemoglobin have been separated on a Partisil-10 ODS-2 column with a linear gradient of acetonitrile containing 0.1% trifluoroacetic acid. The elution profile of the tryptic peptides of the chains obtained with this acetonitrile trifluoroacetate system has been compared with that obtained using phosphoric acid as the ion-pairing reagent. This comparison demonstrated that trifluoroacetate influences the retention times of the histidine-containing tryptic peptides much more than it affects those peptides that do not contain histidine residues. This behavior has been rationalized on the basis of ion-pairing of trifluoroacetate with the histidine residues of the tryptic peptides.

UR - http://www.scopus.com/inward/record.url?scp=0020787358&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0020787358&partnerID=8YFLogxK

M3 - Article

C2 - 6885251

AN - SCOPUS:0020787358

VL - 22

SP - 78

EP - 82

JO - International Journal of Peptide and Protein Research

JF - International Journal of Peptide and Protein Research

SN - 0367-8377

IS - 1

ER -