Influence of trifluoroacetic acid on retention times of histidine‐containing tryptic peptides in reverse phase HPLC

A. SEETHARAMA ACHARYA; ALBERTO DI DONATO; BELUR N. MANJULA; VINCENT A. FISCHETTI; JAMES M. MANNING

doi:10.1111/j.1399-3011.1983.tb02071.x

Influence of trifluoroacetic acid on retention times of histidine‐containing tryptic peptides in reverse phase HPLC

A. SEETHARAMA ACHARYA, ALBERTO DI DONATO, BELUR N. MANJULA, VINCENT A. FISCHETTI, JAMES M. MANNING

Medicine

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Abstract

The tryptic peptides of the aminoethylated α‐and β‐chain of hemoglobin have been separated on a Partisil‐10 ODS‐2 column with a linear gradient of acetonitrile containing 0.1% trifluoroacetic acid. The elution profile of the tryptic peptides of the chains obtained with this acetonitrile trifluoroacetate system has been compared with that obtained using phosphoric acid as the ion‐pairing reagent. This comparison demonstrated that trifluoroacetate influences the retention times of the histidine‐containing tryptic peptides much more than it affects those peptides that do not contain histidine residues. This behavior has been rationalized on the basis of ion‐pairing of trifluoroacetate with the histidine residues of the tryptic peptides.

Original language	English (US)
Pages (from-to)	78-82
Number of pages	5
Journal	International Journal of Peptide and Protein Research
Volume	22
Issue number	1
DOIs	https://doi.org/10.1111/j.1399-3011.1983.tb02071.x
State	Published - Jul 1983

Keywords

hemoglobin
ion‐pairing
trifluoroacetate

ASJC Scopus subject areas

Biochemistry

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10.1111/j.1399-3011.1983.tb02071.x

Cite this

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title = "Influence of trifluoroacetic acid on retention times of histidine‐containing tryptic peptides in reverse phase HPLC",

abstract = "The tryptic peptides of the aminoethylated α‐and β‐chain of hemoglobin have been separated on a Partisil‐10 ODS‐2 column with a linear gradient of acetonitrile containing 0.1% trifluoroacetic acid. The elution profile of the tryptic peptides of the chains obtained with this acetonitrile trifluoroacetate system has been compared with that obtained using phosphoric acid as the ion‐pairing reagent. This comparison demonstrated that trifluoroacetate influences the retention times of the histidine‐containing tryptic peptides much more than it affects those peptides that do not contain histidine residues. This behavior has been rationalized on the basis of ion‐pairing of trifluoroacetate with the histidine residues of the tryptic peptides.",

keywords = "hemoglobin, ion‐pairing, trifluoroacetate",

author = "ACHARYA, {A. SEETHARAMA} and {DI DONATO}, ALBERTO and MANJULA, {BELUR N.} and FISCHETTI, {VINCENT A.} and MANNING, {JAMES M.}",

year = "1983",

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T1 - Influence of trifluoroacetic acid on retention times of histidine‐containing tryptic peptides in reverse phase HPLC

AU - ACHARYA, A. SEETHARAMA

AU - DI DONATO, ALBERTO

AU - MANJULA, BELUR N.

AU - FISCHETTI, VINCENT A.

AU - MANNING, JAMES M.

PY - 1983/7

Y1 - 1983/7

N2 - The tryptic peptides of the aminoethylated α‐and β‐chain of hemoglobin have been separated on a Partisil‐10 ODS‐2 column with a linear gradient of acetonitrile containing 0.1% trifluoroacetic acid. The elution profile of the tryptic peptides of the chains obtained with this acetonitrile trifluoroacetate system has been compared with that obtained using phosphoric acid as the ion‐pairing reagent. This comparison demonstrated that trifluoroacetate influences the retention times of the histidine‐containing tryptic peptides much more than it affects those peptides that do not contain histidine residues. This behavior has been rationalized on the basis of ion‐pairing of trifluoroacetate with the histidine residues of the tryptic peptides.

AB - The tryptic peptides of the aminoethylated α‐and β‐chain of hemoglobin have been separated on a Partisil‐10 ODS‐2 column with a linear gradient of acetonitrile containing 0.1% trifluoroacetic acid. The elution profile of the tryptic peptides of the chains obtained with this acetonitrile trifluoroacetate system has been compared with that obtained using phosphoric acid as the ion‐pairing reagent. This comparison demonstrated that trifluoroacetate influences the retention times of the histidine‐containing tryptic peptides much more than it affects those peptides that do not contain histidine residues. This behavior has been rationalized on the basis of ion‐pairing of trifluoroacetate with the histidine residues of the tryptic peptides.

KW - hemoglobin

KW - ion‐pairing

KW - trifluoroacetate

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Influence of trifluoroacetic acid on retention times of histidine‐containing tryptic peptides in reverse phase HPLC

Abstract

Keywords

ASJC Scopus subject areas

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