Influence of rheologic changes and platelet-neutrophil interactions on cell filtration in sepsis

Linda A. Kirschenbaum, Mohammed Aziz, Mark E. Astiz, Dhanonjoy C. Saha, Eric C. Rackow

Research output: Contribution to journalArticle

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Abstract

We examined the role of erythrocyte (red blood cell; RBC) aggregation and deformability, neutrophil (polymorphonuclear neutrophil; PMN) deformability, whole-blood viscosity, and platelet-neutrophil interactions on cell filtration in subjects who were critically ill with sepsis (CIS), critically ill noninfected subjects (CINS), and healthy controls (C). We assessed cell deformability by filtration through filters of 5-μm pore size. Whole blood, RBC, PMN, and combinations of PMN and RBC were studied. Viscometry was done on isolated RBC. Platelet-PMN interactions were assessed with monoclonal antibodies to CD41 and activated CD53 platelet receptors, and to CD66b PMN receptors. Filtration pressure (Pi) for CIS was significantly greater than for C and CINS at both high and low PMN and RBC concentrations. Viscometry confirmed decreases in RBC deformability and demonstrated significant increases in RBC aggregation in CIS. Increments in Pi were significantly greater with PMN and PMN-RBC combinations suspended in platelet rich plasma (PRP) than in platelet poor plasma (PPP) for CIS as compared with CINS or C. Flow cytometry confirmed significantly greater platelet activation in CIS than in CINS or C (mean fluorescence: 39 ± 9 Ifu versus 18.7 ± 4.0 Ifu and 17.1 ± 2.3 Ifu, respectively) and greater platelet-PMN aggregation (mean fluorescence: 44.7 ± 3.6 Ifu versus 23 ± 4.1 Ifu, respectively) in CIS than in C. We conclude that decreased filtration of whole blood in CIS is related to decreases in RBC and PMN deformability, increases in RBC aggregation, and increased platelet-PMN interactions. Of these, the formation of platelet-PMN aggregates appeares to have the greatest effect in impairing cell filtration. These rheologic abnormalities may contribute to impaired microvascular blood flow in patients with sepsis.

Original languageEnglish (US)
Pages (from-to)1602-1607
Number of pages6
JournalAmerican Journal of Respiratory and Critical Care Medicine
Volume161
Issue number5
StatePublished - 2000
Externally publishedYes

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Critical Illness
Cell Communication
Sepsis
Neutrophils
Blood Platelets
Platelet Aggregation
Erythrocytes
Fluorescence
Cell Aggregation
Blood Viscosity
Platelet-Rich Plasma
Platelet Activation
Healthy Volunteers
Flow Cytometry
Monoclonal Antibodies
Pressure

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine

Cite this

Influence of rheologic changes and platelet-neutrophil interactions on cell filtration in sepsis. / Kirschenbaum, Linda A.; Aziz, Mohammed; Astiz, Mark E.; Saha, Dhanonjoy C.; Rackow, Eric C.

In: American Journal of Respiratory and Critical Care Medicine, Vol. 161, No. 5, 2000, p. 1602-1607.

Research output: Contribution to journalArticle

Kirschenbaum, Linda A. ; Aziz, Mohammed ; Astiz, Mark E. ; Saha, Dhanonjoy C. ; Rackow, Eric C. / Influence of rheologic changes and platelet-neutrophil interactions on cell filtration in sepsis. In: American Journal of Respiratory and Critical Care Medicine. 2000 ; Vol. 161, No. 5. pp. 1602-1607.
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