Influence of neutropenia on the course of serotype 8 pneumococcal pneumonia in mice

Matthew Marks, Tamika Burns, Maria Abadi, Beza Seyoum, Justin Thornton, Elaine Tuomanen, Liise-anne Pirofski

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Abstract

Polymorphoneutrophils (PMNs) are important effector cells in host defense against pneumonia. However, PMNs can also induce inflammation and tissue damage. To investigate the contribution of PMNs to host defense against pneumococcal pneumonia, we determined the effect of the PMN-depleting rat monoclonal antibody MB6-8C5 (RB6) on survival and inflammatory and cellular response in the lungs to a lethal intranasal infection with a serotype 8 pneumococcus in BALB/c mice. Control mice received rat immunoglobulin G (rIgG). Strikingly, the survival of RB6-treatcd mice was significantly prolonged compared to that of rIgG-treated mice. Although the numbers of CFU in the lungs were statistically similar in both groups 4, 24, and 32 h after infection, rIgG-treated mice developed higher levels of bacteremia, and histopathological examination of the longs of infected mice revealed marked differences between MB6- and rIgG-treated mice. RB6-treated mice had focal, perivascular lesions without accompanying parenchymal inflammation, and rIgG-treated mice had diffuse, interstitial parenchymal inflammation. Lung homogenates from the rIgG-treated mice had more leukocytes and significantly more total and apoptotic PMNs as determined by fluorescence-activated cell sorter analysis with Annexin V and terminal deoxynucleotidyltransferase-mediated dUTP-biotin nick end labeling staining of lung tissue samples. Studies with a pneumolysin-deficient mutant of the serotype 8 strain we used also demonstrated the prolonged survival of RB6- compared to rIgG-treated mice. Taken together, our findings suggest that PMNs enhance the likelihood of early death and alter the pathological response to pneumococcal lung infection in BALB/c mice with serotype 8 pneumonia without significantly affecting bacterial clearance or the cytokine response.

Original languageEnglish (US)
Pages (from-to)1586-1597
Number of pages12
JournalInfection and Immunity
Volume75
Issue number4
DOIs
StatePublished - Apr 2007

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Pneumococcal Pneumonia
Neutropenia
Immunoglobulin G
Lung
Inflammation
Serogroup
Pneumonia
Pneumococcal Infections
DNA Nucleotidylexotransferase
Annexin A5
Biotin
Bacteremia
Streptococcus pneumoniae
Infection

ASJC Scopus subject areas

  • Immunology

Cite this

Influence of neutropenia on the course of serotype 8 pneumococcal pneumonia in mice. / Marks, Matthew; Burns, Tamika; Abadi, Maria; Seyoum, Beza; Thornton, Justin; Tuomanen, Elaine; Pirofski, Liise-anne.

In: Infection and Immunity, Vol. 75, No. 4, 04.2007, p. 1586-1597.

Research output: Contribution to journalArticle

Marks, M, Burns, T, Abadi, M, Seyoum, B, Thornton, J, Tuomanen, E & Pirofski, L 2007, 'Influence of neutropenia on the course of serotype 8 pneumococcal pneumonia in mice', Infection and Immunity, vol. 75, no. 4, pp. 1586-1597. https://doi.org/10.1128/IAI.01579-06
Marks M, Burns T, Abadi M, Seyoum B, Thornton J, Tuomanen E et al. Influence of neutropenia on the course of serotype 8 pneumococcal pneumonia in mice. Infection and Immunity. 2007 Apr;75(4):1586-1597. https://doi.org/10.1128/IAI.01579-06
Marks, Matthew ; Burns, Tamika ; Abadi, Maria ; Seyoum, Beza ; Thornton, Justin ; Tuomanen, Elaine ; Pirofski, Liise-anne. / Influence of neutropenia on the course of serotype 8 pneumococcal pneumonia in mice. In: Infection and Immunity. 2007 ; Vol. 75, No. 4. pp. 1586-1597.
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