Influence of Cl- on organic anion transport in short-term cultured rat hepatocytes and isolated perfused rat liver

Allan W. Wolkoff, A. C. Samuelson, K. L. Johansen, R. Nakata, D. M. Withers, A. Sosiak

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Abstract

Transport of 35S-labeled sulfobromophthalein [35S]BSP was studied in short-term cultured rat hepatocytes incubated in bovine serum albumin. At 37°C, initial uptake of [35S]BSP was 5-10-fold that at 4°C, linear for at least 15 min, saturable, inhibited by bilirubin, and reduced by > 70% after ATP depletion or isosmotic substitution of sucrose for NaCl in medium. Replacement of Na+ by K+ or Li+ did not alter uptake, whereas replacement of Cl- by HCO3- or gluconate- reduced uptake by ~ 40%. Substitution of Cl- by the more permeant NO3- enhanced initial BSP uptake by 30%. Efflux of [35S]BSP from cells to media was inhibited by 40% after ATP depletion or sucrose substitution. To confirm these results in a more physiologic system, transport of [3H]bilirubin was studied in isolated livers perfused with control medium or medium in which Cl- was replaced by gluconate-. Perfusion data analyzed by the model of Goresky, revealed 40-50% reductions in influx and efflux with gluconate- substitution. These results are consistent with existence of a Cl-/organic anion-exchange mechanism similar to that described by others in renal tubules.

Original languageEnglish (US)
Pages (from-to)1259-1268
Number of pages10
JournalJournal of Clinical Investigation
Volume79
Issue number4
StatePublished - 1987

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Anions
Hepatocytes
Bilirubin
Sucrose
Liver
Adenosine Triphosphate
Sulfobromophthalein
Bovine Serum Albumin
Perfusion
Kidney
gluconic acid

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Wolkoff, A. W., Samuelson, A. C., Johansen, K. L., Nakata, R., Withers, D. M., & Sosiak, A. (1987). Influence of Cl- on organic anion transport in short-term cultured rat hepatocytes and isolated perfused rat liver. Journal of Clinical Investigation, 79(4), 1259-1268.

Influence of Cl- on organic anion transport in short-term cultured rat hepatocytes and isolated perfused rat liver. / Wolkoff, Allan W.; Samuelson, A. C.; Johansen, K. L.; Nakata, R.; Withers, D. M.; Sosiak, A.

In: Journal of Clinical Investigation, Vol. 79, No. 4, 1987, p. 1259-1268.

Research output: Contribution to journalArticle

Wolkoff, AW, Samuelson, AC, Johansen, KL, Nakata, R, Withers, DM & Sosiak, A 1987, 'Influence of Cl- on organic anion transport in short-term cultured rat hepatocytes and isolated perfused rat liver', Journal of Clinical Investigation, vol. 79, no. 4, pp. 1259-1268.
Wolkoff, Allan W. ; Samuelson, A. C. ; Johansen, K. L. ; Nakata, R. ; Withers, D. M. ; Sosiak, A. / Influence of Cl- on organic anion transport in short-term cultured rat hepatocytes and isolated perfused rat liver. In: Journal of Clinical Investigation. 1987 ; Vol. 79, No. 4. pp. 1259-1268.
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