Inflammatory monocytes and neutrophils are licensed to kill during memory responses in vivo

Emilie Narni-Mancinelli, Saidi M Homa Soudja, Karine Crozat, Marc Dalod, Pierre Gounon, Frédéric Geissmann, Gregoire Lauvau

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

Immunological memory is a hallmark of B and T lymphocytes that have undergone a previous encounter with a given antigen. It is assumed that memory cells mediate better protection of the host upon re-infection because of improved effector functions such as antibody production, cytotoxic activity and cytokine secretion. In contrast to cells of the adaptive immune system, innate immune cells are believed to exhibit a comparable functional effector response each time the same pathogen is encountered. Here, using mice infected by the intracellular bacterium Listeria monocytogenes, we show that during a recall bacterial infection, the chemokine CCL3 secreted by memory CD8+ T cells drives drastic modifications of the functional properties of several populations of phagocytes. We found that inflammatory ly6C+ monocytes and neutrophils largely mediated memory CD8+ T cell bacteriocidal activity by producing increased levels of reactive oxygen species (ROS), augmenting the pH of their phagosomes and inducing antimicrobial autophagy. These events allowed an extremely rapid control of bacterial growth in vivo and accounted for protective immunity. Therefore, our results provide evidence that cytotoxic memory CD8+ T cells can license distinct antimicrobial effector mechanisms of innate cells to efficiently clear pathogens.

Original languageEnglish (US)
Article numbere1002457
JournalPLoS Pathogens
Volume7
Issue number12
DOIs
StatePublished - Dec 2011

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Monocytes
Neutrophils
T-Lymphocytes
Immunologic Memory
Chemokine CCL3
Phagosomes
Autophagy
Listeria monocytogenes
Licensure
Phagocytes
Bacterial Infections
Antibody Formation
Immune System
Immunity
Reactive Oxygen Species
B-Lymphocytes
Cytokines
Bacteria
Antigens
Growth

ASJC Scopus subject areas

  • Microbiology
  • Parasitology
  • Virology
  • Immunology
  • Genetics
  • Molecular Biology

Cite this

Narni-Mancinelli, E., Soudja, S. M. H., Crozat, K., Dalod, M., Gounon, P., Geissmann, F., & Lauvau, G. (2011). Inflammatory monocytes and neutrophils are licensed to kill during memory responses in vivo. PLoS Pathogens, 7(12), [e1002457]. https://doi.org/10.1371/journal.ppat.1002457

Inflammatory monocytes and neutrophils are licensed to kill during memory responses in vivo. / Narni-Mancinelli, Emilie; Soudja, Saidi M Homa; Crozat, Karine; Dalod, Marc; Gounon, Pierre; Geissmann, Frédéric; Lauvau, Gregoire.

In: PLoS Pathogens, Vol. 7, No. 12, e1002457, 12.2011.

Research output: Contribution to journalArticle

Narni-Mancinelli, E, Soudja, SMH, Crozat, K, Dalod, M, Gounon, P, Geissmann, F & Lauvau, G 2011, 'Inflammatory monocytes and neutrophils are licensed to kill during memory responses in vivo', PLoS Pathogens, vol. 7, no. 12, e1002457. https://doi.org/10.1371/journal.ppat.1002457
Narni-Mancinelli E, Soudja SMH, Crozat K, Dalod M, Gounon P, Geissmann F et al. Inflammatory monocytes and neutrophils are licensed to kill during memory responses in vivo. PLoS Pathogens. 2011 Dec;7(12). e1002457. https://doi.org/10.1371/journal.ppat.1002457
Narni-Mancinelli, Emilie ; Soudja, Saidi M Homa ; Crozat, Karine ; Dalod, Marc ; Gounon, Pierre ; Geissmann, Frédéric ; Lauvau, Gregoire. / Inflammatory monocytes and neutrophils are licensed to kill during memory responses in vivo. In: PLoS Pathogens. 2011 ; Vol. 7, No. 12.
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