Inflammatory mediators are induced by dietary glycotoxins, a major risk factor for diabetic angiopathy

Helen Vlassara, Weijing Cai, Jill P. Crandall, Teresia Goldberg, Robert Oberstein, Veronique Dardaine, Melpomeni Peppa, Elliot J. Rayfield

Research output: Contribution to journalArticle

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Abstract

Diet is a major environmental source of proinflammatory AGEs (heat-generated advanced glycation end products); its impact in humans remains unclear. We explored the effects of two equivalent diets, one regular (high AGE, H-AGE) and the other with 5-fold lower AGE (L-AGE) content on inflammatory mediators of 24 diabetic subjects: 11 in a 2-week crossover and 13 in a 6-week study. After 2 weeks on H-AGE, serum AGEs increased by 64.5% (P = 0.02) and on L-AGE decreased by 30% (P = 0.02). The mononuclear cell tumor necrosis factor-α/β-actin mRNA ratio was 1.4 ± 0.5 on H-AGE and 0.9 ± 0.5 on L-AGE (P = 0.05), whereas serum vascular adhesion molecule-1 was 1,108 ± 429 and 698 ± 347 ng/ml (P = 0.01) on L- and H-AGE, respectively. After 6 weeks, peripheral blood mononuclear cell tumor necrosis factor-α rose by 86.3% (P = 0.006) and declined by 20% (P, not significant) on H- or L-AGE diet, respectively; C-reactive protein increased by 35% on H-AGE and decreased by 20% on L-AGE (P = 0.014), and vascular adhesion molecule-1 declined by 20% on L-AGE (P < 0.01) and increased by 4% on H-AGE. Serum AGEs were increased by 28.2% on H-AGE (P = 0.06) and reduced by 40% on L-AGE (P = 0.02), whereas AGE low density lipoprotein was increased by 32% on H-AGE and reduced by 33% on L-AGE diet (P < 0.05). Thus in diabetes, environmental (dietary) AGEs promote inflammatory mediators, leading to tissue injury. Restriction of dietary AGEs suppresses these effects.

Original languageEnglish (US)
Pages (from-to)15596-15601
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume99
Issue number24
DOIs
StatePublished - Nov 26 2002
Externally publishedYes

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Diabetic Angiopathies
Diet
Blood Vessels
Tumor Necrosis Factor-alpha
Serum
Advanced Glycosylation End Products
LDL Lipoproteins
C-Reactive Protein
Actins
Blood Cells
Hot Temperature
Messenger RNA
Wounds and Injuries

ASJC Scopus subject areas

  • Genetics
  • General

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Inflammatory mediators are induced by dietary glycotoxins, a major risk factor for diabetic angiopathy. / Vlassara, Helen; Cai, Weijing; Crandall, Jill P.; Goldberg, Teresia; Oberstein, Robert; Dardaine, Veronique; Peppa, Melpomeni; Rayfield, Elliot J.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, No. 24, 26.11.2002, p. 15596-15601.

Research output: Contribution to journalArticle

Vlassara, Helen ; Cai, Weijing ; Crandall, Jill P. ; Goldberg, Teresia ; Oberstein, Robert ; Dardaine, Veronique ; Peppa, Melpomeni ; Rayfield, Elliot J. / Inflammatory mediators are induced by dietary glycotoxins, a major risk factor for diabetic angiopathy. In: Proceedings of the National Academy of Sciences of the United States of America. 2002 ; Vol. 99, No. 24. pp. 15596-15601.
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AU - Vlassara, Helen

AU - Cai, Weijing

AU - Crandall, Jill P.

AU - Goldberg, Teresia

AU - Oberstein, Robert

AU - Dardaine, Veronique

AU - Peppa, Melpomeni

AU - Rayfield, Elliot J.

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