Inflammatory Flt3l is essential to mobilize dendritic cells and for T cell responses during Plasmodium infection

Pierre Guermonprez, Julie Helft, Carla Claser, Stephanie Deroubaix, Henry Karanje, Anna Gazumyan, Guillaume Darasse-Jèze, Stephanie B. Telerman, Gaëlle Breton, Heidi A. Schreiber, Natalia Frias-Staheli, Eva K. Billerbeck, Marcus Dorner, Charles M. Rice, Alexander Ploss, Florian Klein, Melissa Swiecki, Marco Colonna, Alice O. Kamphorst, Matthew Meredith & 12 others Rachel Niec, Constantin Takacs, Fadi Mikhail, Aswin Hari, David Bosque, Tom Eisenreich, Miriam Merad, Yan Shi, Florent Ginhoux, Laurent Rénia, Britta C. Urban, Michel C. Nussenzweig

Research output: Contribution to journalArticle

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Abstract

Innate sensing mechanisms trigger a variety of humoral and cellular events that are essential to adaptive immune responses. Here we describe an innate sensing pathway triggered by Plasmodium infection that regulates dendritic cell homeostasis and adaptive immunity through Flt3 ligand (Flt3l) release. Plasmodium-induced Flt3l release in mice requires Toll-like receptor (TLR) activation and type I interferon (IFN) production. We found that type I IFN supports the upregulation of xanthine dehydrogenase, which metabolizes the xanthine accumulating in infected erythrocytes to uric acid. Uric acid crystals trigger mast cells to release soluble Flt3l from a pre-synthesized membrane-associated precursor. During infection, Flt3l preferentially stimulates expansion of the CD8-α+ dendritic cell subset or its BDCA3+ human dendritic cell equivalent and has a substantial impact on the magnitude of T cell activation, mostly in the CD8+ compartment. Our findings highlight a new mechanism that regulates dendritic cell homeostasis and T cell responses to infection.

Original languageEnglish (US)
Pages (from-to)730-738
Number of pages9
JournalNature Medicine
Volume19
Issue number6
DOIs
StatePublished - Jun 1 2013
Externally publishedYes

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T-cells
Dendritic Cells
Malaria
T-Lymphocytes
Interferon Type I
Adaptive Immunity
Uric Acid
Homeostasis
Chemical activation
Xanthine Dehydrogenase
Xanthine
Plasmodium
Toll-Like Receptors
Infection
Mast Cells
Up-Regulation
Erythrocytes
Membranes
Crystals
flt3 ligand protein

ASJC Scopus subject areas

  • Medicine(all)
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Guermonprez, P., Helft, J., Claser, C., Deroubaix, S., Karanje, H., Gazumyan, A., ... Nussenzweig, M. C. (2013). Inflammatory Flt3l is essential to mobilize dendritic cells and for T cell responses during Plasmodium infection. Nature Medicine, 19(6), 730-738. https://doi.org/10.1038/nm.3197

Inflammatory Flt3l is essential to mobilize dendritic cells and for T cell responses during Plasmodium infection. / Guermonprez, Pierre; Helft, Julie; Claser, Carla; Deroubaix, Stephanie; Karanje, Henry; Gazumyan, Anna; Darasse-Jèze, Guillaume; Telerman, Stephanie B.; Breton, Gaëlle; Schreiber, Heidi A.; Frias-Staheli, Natalia; Billerbeck, Eva K.; Dorner, Marcus; Rice, Charles M.; Ploss, Alexander; Klein, Florian; Swiecki, Melissa; Colonna, Marco; Kamphorst, Alice O.; Meredith, Matthew; Niec, Rachel; Takacs, Constantin; Mikhail, Fadi; Hari, Aswin; Bosque, David; Eisenreich, Tom; Merad, Miriam; Shi, Yan; Ginhoux, Florent; Rénia, Laurent; Urban, Britta C.; Nussenzweig, Michel C.

In: Nature Medicine, Vol. 19, No. 6, 01.06.2013, p. 730-738.

Research output: Contribution to journalArticle

Guermonprez, P, Helft, J, Claser, C, Deroubaix, S, Karanje, H, Gazumyan, A, Darasse-Jèze, G, Telerman, SB, Breton, G, Schreiber, HA, Frias-Staheli, N, Billerbeck, EK, Dorner, M, Rice, CM, Ploss, A, Klein, F, Swiecki, M, Colonna, M, Kamphorst, AO, Meredith, M, Niec, R, Takacs, C, Mikhail, F, Hari, A, Bosque, D, Eisenreich, T, Merad, M, Shi, Y, Ginhoux, F, Rénia, L, Urban, BC & Nussenzweig, MC 2013, 'Inflammatory Flt3l is essential to mobilize dendritic cells and for T cell responses during Plasmodium infection', Nature Medicine, vol. 19, no. 6, pp. 730-738. https://doi.org/10.1038/nm.3197
Guermonprez P, Helft J, Claser C, Deroubaix S, Karanje H, Gazumyan A et al. Inflammatory Flt3l is essential to mobilize dendritic cells and for T cell responses during Plasmodium infection. Nature Medicine. 2013 Jun 1;19(6):730-738. https://doi.org/10.1038/nm.3197
Guermonprez, Pierre ; Helft, Julie ; Claser, Carla ; Deroubaix, Stephanie ; Karanje, Henry ; Gazumyan, Anna ; Darasse-Jèze, Guillaume ; Telerman, Stephanie B. ; Breton, Gaëlle ; Schreiber, Heidi A. ; Frias-Staheli, Natalia ; Billerbeck, Eva K. ; Dorner, Marcus ; Rice, Charles M. ; Ploss, Alexander ; Klein, Florian ; Swiecki, Melissa ; Colonna, Marco ; Kamphorst, Alice O. ; Meredith, Matthew ; Niec, Rachel ; Takacs, Constantin ; Mikhail, Fadi ; Hari, Aswin ; Bosque, David ; Eisenreich, Tom ; Merad, Miriam ; Shi, Yan ; Ginhoux, Florent ; Rénia, Laurent ; Urban, Britta C. ; Nussenzweig, Michel C. / Inflammatory Flt3l is essential to mobilize dendritic cells and for T cell responses during Plasmodium infection. In: Nature Medicine. 2013 ; Vol. 19, No. 6. pp. 730-738.
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