Inferring transient particle transport dynamics in live cells

Nilah Monnier; Zachary Barry; Hye Yoon Park; Kuan Chung Su; Zachary Katz; Brian P. English; Arkajit Dey; Keyao Pan; Iain M. Cheeseman; Robert H. Singer; Mark Bathe

doi:10.1038/nmeth.3483

Inferring transient particle transport dynamics in live cells

Nilah Monnier, Zachary Barry, Hye Yoon Park, Kuan Chung Su, Zachary Katz, Brian P. English, Arkajit Dey, Keyao Pan, Iain M. Cheeseman, Robert H. Singer, Mark Bathe

Cell Biology

Research output: Contribution to journal › Article › peer-review

113 Scopus citations

Abstract

Live-cell imaging and particle tracking provide rich information on mechanisms of intracellular transport. However, trajectory analysis procedures to infer complex transport dynamics involving stochastic switching between active transport and diffusive motion are lacking. We applied Bayesian model selection to hidden Markov modeling to infer transient transport states from trajectories of mRNA-protein complexes in live mouse hippocampal neurons and metaphase kinetochores in dividing human cells.

Original language	English (US)
Pages (from-to)	838-840
Number of pages	3
Journal	Nature Methods
Volume	12
Issue number	9
DOIs	https://doi.org/10.1038/nmeth.3483
State	Published - Aug 28 2015

ASJC Scopus subject areas

Biotechnology
Biochemistry
Molecular Biology
Cell Biology

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10.1038/nmeth.3483

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abstract = "Live-cell imaging and particle tracking provide rich information on mechanisms of intracellular transport. However, trajectory analysis procedures to infer complex transport dynamics involving stochastic switching between active transport and diffusive motion are lacking. We applied Bayesian model selection to hidden Markov modeling to infer transient transport states from trajectories of mRNA-protein complexes in live mouse hippocampal neurons and metaphase kinetochores in dividing human cells.",

author = "Nilah Monnier and Zachary Barry and Park, {Hye Yoon} and Su, {Kuan Chung} and Zachary Katz and English, {Brian P.} and Arkajit Dey and Keyao Pan and Cheeseman, {Iain M.} and Singer, {Robert H.} and Mark Bathe",

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AU - Monnier, Nilah

AU - Barry, Zachary

AU - Park, Hye Yoon

AU - Su, Kuan Chung

AU - Katz, Zachary

AU - English, Brian P.

AU - Dey, Arkajit

AU - Pan, Keyao

AU - Cheeseman, Iain M.

AU - Singer, Robert H.

AU - Bathe, Mark

PY - 2015/8/28

Y1 - 2015/8/28

N2 - Live-cell imaging and particle tracking provide rich information on mechanisms of intracellular transport. However, trajectory analysis procedures to infer complex transport dynamics involving stochastic switching between active transport and diffusive motion are lacking. We applied Bayesian model selection to hidden Markov modeling to infer transient transport states from trajectories of mRNA-protein complexes in live mouse hippocampal neurons and metaphase kinetochores in dividing human cells.

AB - Live-cell imaging and particle tracking provide rich information on mechanisms of intracellular transport. However, trajectory analysis procedures to infer complex transport dynamics involving stochastic switching between active transport and diffusive motion are lacking. We applied Bayesian model selection to hidden Markov modeling to infer transient transport states from trajectories of mRNA-protein complexes in live mouse hippocampal neurons and metaphase kinetochores in dividing human cells.

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JO - Nature Methods

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Inferring transient particle transport dynamics in live cells

Abstract

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