Inferring transient particle transport dynamics in live cells

Nilah Monnier, Zachary Barry, Hye Yoon Park, Kuan Chung Su, Zachary Katz, Brian P. English, Arkajit Dey, Keyao Pan, Iain M. Cheeseman, Robert H. Singer, Mark Bathe

Research output: Contribution to journalArticle

60 Citations (Scopus)

Abstract

Live-cell imaging and particle tracking provide rich information on mechanisms of intracellular transport. However, trajectory analysis procedures to infer complex transport dynamics involving stochastic switching between active transport and diffusive motion are lacking. We applied Bayesian model selection to hidden Markov modeling to infer transient transport states from trajectories of mRNA-protein complexes in live mouse hippocampal neurons and metaphase kinetochores in dividing human cells.

Original languageEnglish (US)
Pages (from-to)838-840
Number of pages3
JournalNature Methods
Volume12
Issue number9
DOIs
StatePublished - Aug 28 2015

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Trajectories
Kinetochores
Active Biological Transport
Metaphase
Neurons
Cells
Imaging techniques
Messenger RNA
Proteins

ASJC Scopus subject areas

  • Biotechnology
  • Molecular Biology
  • Biochemistry
  • Cell Biology

Cite this

Monnier, N., Barry, Z., Park, H. Y., Su, K. C., Katz, Z., English, B. P., ... Bathe, M. (2015). Inferring transient particle transport dynamics in live cells. Nature Methods, 12(9), 838-840. https://doi.org/10.1038/nmeth.3483

Inferring transient particle transport dynamics in live cells. / Monnier, Nilah; Barry, Zachary; Park, Hye Yoon; Su, Kuan Chung; Katz, Zachary; English, Brian P.; Dey, Arkajit; Pan, Keyao; Cheeseman, Iain M.; Singer, Robert H.; Bathe, Mark.

In: Nature Methods, Vol. 12, No. 9, 28.08.2015, p. 838-840.

Research output: Contribution to journalArticle

Monnier, N, Barry, Z, Park, HY, Su, KC, Katz, Z, English, BP, Dey, A, Pan, K, Cheeseman, IM, Singer, RH & Bathe, M 2015, 'Inferring transient particle transport dynamics in live cells', Nature Methods, vol. 12, no. 9, pp. 838-840. https://doi.org/10.1038/nmeth.3483
Monnier N, Barry Z, Park HY, Su KC, Katz Z, English BP et al. Inferring transient particle transport dynamics in live cells. Nature Methods. 2015 Aug 28;12(9):838-840. https://doi.org/10.1038/nmeth.3483
Monnier, Nilah ; Barry, Zachary ; Park, Hye Yoon ; Su, Kuan Chung ; Katz, Zachary ; English, Brian P. ; Dey, Arkajit ; Pan, Keyao ; Cheeseman, Iain M. ; Singer, Robert H. ; Bathe, Mark. / Inferring transient particle transport dynamics in live cells. In: Nature Methods. 2015 ; Vol. 12, No. 9. pp. 838-840.
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