Infectious viral DNA of murine leukemia virus

D. Smotkin; A. M. Gianni; S. Rozenblatt; R. A. Weinberg

doi:10.1073/pnas.72.12.4910

Infectious viral DNA of murine leukemia virus

D. Smotkin, A. M. Gianni, S. Rozenblatt, R. A. Weinberg

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35 Scopus citations

Abstract

A fraction of the unintegrated viral DNA that appears early after infection of mouse cells by Moloney leukemia virus is infectious. The infectivity could be demonstrated by an XC plaque assay of the cells exposed to DNA co precipitated with calcium phosphate. The number of plaques deriving from closed circular, supercoiled DNA was proportional to the concentration of added DNA, indicating that a single DNA molecule of about 5.5 x 10⁶ daltons carries all the viral information. Nonsupercoiled viral DNA is also infectious; these molecules appear to be largely double stranded and 5 to 6 x 10⁶ daltons in mass.

Original language	English (US)
Pages (from-to)	4910-4913
Number of pages	4
Journal	Proceedings of the National Academy of Sciences of the United States of America
Volume	72
Issue number	12
DOIs	https://doi.org/10.1073/pnas.72.12.4910
State	Published - 1975
Externally published	Yes

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AU - Smotkin, D.

AU - Gianni, A. M.

AU - Rozenblatt, S.

AU - Weinberg, R. A.

PY - 1975

Y1 - 1975

N2 - A fraction of the unintegrated viral DNA that appears early after infection of mouse cells by Moloney leukemia virus is infectious. The infectivity could be demonstrated by an XC plaque assay of the cells exposed to DNA co precipitated with calcium phosphate. The number of plaques deriving from closed circular, supercoiled DNA was proportional to the concentration of added DNA, indicating that a single DNA molecule of about 5.5 x 106 daltons carries all the viral information. Nonsupercoiled viral DNA is also infectious; these molecules appear to be largely double stranded and 5 to 6 x 106 daltons in mass.

AB - A fraction of the unintegrated viral DNA that appears early after infection of mouse cells by Moloney leukemia virus is infectious. The infectivity could be demonstrated by an XC plaque assay of the cells exposed to DNA co precipitated with calcium phosphate. The number of plaques deriving from closed circular, supercoiled DNA was proportional to the concentration of added DNA, indicating that a single DNA molecule of about 5.5 x 106 daltons carries all the viral information. Nonsupercoiled viral DNA is also infectious; these molecules appear to be largely double stranded and 5 to 6 x 106 daltons in mass.

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Infectious viral DNA of murine leukemia virus

Abstract

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