Infection of organotypic cultures of spinal cord and dorsal root ganglia with Trypanosoma cruzi

H. B. Tanowitz, C. Brosnan, D. Guastamacchio, G. Baron, C. Raventos-Suarez, M. Bornstein, M. Wittner

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Although the involvement of the nervous system in Chagas' disease is well described, the mechanism of the neuronal destruction is unclear. Immunologic, toxic mechanisms and direct invasion have been advocated. Organotypic cultures of spinal cord and dorsal root ganglion derived from Swiss outbred mice were infected with the Brazil strain of Trypanosoma cruzi. Light microscopic and ultrastructural studies were performed at regular intervals. It was found that trypomastigotes were rapidly taken up by glial and other supporting cells. Neurons were rarely parasitized and demyelination was not evident. Loss of several cytoskeletal components was seen. Dendrites were swollen and axons lost their normal filamentous structures but synaptic membranes remained intact. Mitochondrial swelling was evident even in nonparasitized neurons from infected cultures. By 7-10 days of infection the majority of neurons lost their typical morphology and were eventually destroyed by mechanisms other than direct parasite invasion. Organotypic cultures exposed to T. cruzi-conditioned medium exhibited no change in morphology. Since neurons were found only rarely to be parasitized, it is suggested that neuronal destruction is an indirect result of the parasitism of supporting cells such as glial cells and macrophages.

Original languageEnglish (US)
Pages (from-to)1090-1097
Number of pages8
JournalAmerican Journal of Tropical Medicine and Hygiene
Volume31
Issue number6
StatePublished - 1982

Fingerprint

Spinal Nerve Roots
Trypanosoma cruzi
Spinal Ganglia
Spinal Cord
Neurons
Infection
Neuroglia
Mitochondrial Swelling
Synaptic Membranes
Chagas Disease
Poisons
Demyelinating Diseases
Conditioned Culture Medium
Dendrites
Nervous System
Brazil
Axons
Parasites
Macrophages
Light

ASJC Scopus subject areas

  • Parasitology
  • Infectious Diseases

Cite this

Tanowitz, H. B., Brosnan, C., Guastamacchio, D., Baron, G., Raventos-Suarez, C., Bornstein, M., & Wittner, M. (1982). Infection of organotypic cultures of spinal cord and dorsal root ganglia with Trypanosoma cruzi. American Journal of Tropical Medicine and Hygiene, 31(6), 1090-1097.

Infection of organotypic cultures of spinal cord and dorsal root ganglia with Trypanosoma cruzi. / Tanowitz, H. B.; Brosnan, C.; Guastamacchio, D.; Baron, G.; Raventos-Suarez, C.; Bornstein, M.; Wittner, M.

In: American Journal of Tropical Medicine and Hygiene, Vol. 31, No. 6, 1982, p. 1090-1097.

Research output: Contribution to journalArticle

Tanowitz, HB, Brosnan, C, Guastamacchio, D, Baron, G, Raventos-Suarez, C, Bornstein, M & Wittner, M 1982, 'Infection of organotypic cultures of spinal cord and dorsal root ganglia with Trypanosoma cruzi', American Journal of Tropical Medicine and Hygiene, vol. 31, no. 6, pp. 1090-1097.
Tanowitz HB, Brosnan C, Guastamacchio D, Baron G, Raventos-Suarez C, Bornstein M et al. Infection of organotypic cultures of spinal cord and dorsal root ganglia with Trypanosoma cruzi. American Journal of Tropical Medicine and Hygiene. 1982;31(6):1090-1097.
Tanowitz, H. B. ; Brosnan, C. ; Guastamacchio, D. ; Baron, G. ; Raventos-Suarez, C. ; Bornstein, M. ; Wittner, M. / Infection of organotypic cultures of spinal cord and dorsal root ganglia with Trypanosoma cruzi. In: American Journal of Tropical Medicine and Hygiene. 1982 ; Vol. 31, No. 6. pp. 1090-1097.
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