Infection of mice with aerosolized Mycobacterium tuberculosis: Use of a nose-only apparatus for delivery of low doses of inocula and design of an ultrasafe facility

J. Reid Schwebach; Bing Chen; Aharona Glatman-Freedman; Arturo Casadevall; John D. McKinney; John L. Harb; Patrick J. McGuire; W. Emmett Barkley; Barry R. Bloom; William R. Jacobs

doi:10.1128/AEM.68.9.4646-4649.2002

Infection of mice with aerosolized Mycobacterium tuberculosis: Use of a nose-only apparatus for delivery of low doses of inocula and design of an ultrasafe facility

J. Reid Schwebach, Bing Chen, Aharona Glatman-Freedman, Arturo Casadevall, John D. McKinney, John L. Harb, Patrick J. McGuire, W. Emmett Barkley, Barry R. Bloom, William R. Jacobs

Microbiology & Immunology

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

Aerosolized delivery of virulent or hypervirulent Mycobacterium tuberculosis requires careful consideration of methodology and safety. To maximize safety, we installed a nose-only aerosol apparatus that can reproducibly deliver a low dose (<100 CFU per mouse) of M. tuberculosis in a carefully designed biohazard facility.

Original language	English (US)
Pages (from-to)	4646-4649
Number of pages	4
Journal	Applied and Environmental Microbiology
Volume	68
Issue number	9
DOIs	https://doi.org/10.1128/AEM.68.9.4646-4649.2002
State	Published - Sep 2002

ASJC Scopus subject areas

Biotechnology
Food Science
Ecology
Applied Microbiology and Biotechnology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1128/AEM.68.9.4646-4649.2002

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Schwebach, J. R., Chen, B., Glatman-Freedman, A., Casadevall, A., McKinney, J. D., Harb, J. L., McGuire, P. J., Barkley, W. E., Bloom, B. R., & Jacobs, W. R. (2002). Infection of mice with aerosolized Mycobacterium tuberculosis: Use of a nose-only apparatus for delivery of low doses of inocula and design of an ultrasafe facility. Applied and Environmental Microbiology, 68(9), 4646-4649. https://doi.org/10.1128/AEM.68.9.4646-4649.2002

Infection of mice with aerosolized Mycobacterium tuberculosis: Use of a nose-only apparatus for delivery of low doses of inocula and design of an ultrasafe facility. / Schwebach, J. Reid; Chen, Bing; Glatman-Freedman, Aharona et al.
In: Applied and Environmental Microbiology, Vol. 68, No. 9, 09.2002, p. 4646-4649.

Research output: Contribution to journal › Article › peer-review

Schwebach, JR, Chen, B, Glatman-Freedman, A, Casadevall, A, McKinney, JD, Harb, JL, McGuire, PJ, Barkley, WE, Bloom, BR & Jacobs, WR 2002, 'Infection of mice with aerosolized Mycobacterium tuberculosis: Use of a nose-only apparatus for delivery of low doses of inocula and design of an ultrasafe facility', Applied and Environmental Microbiology, vol. 68, no. 9, pp. 4646-4649. https://doi.org/10.1128/AEM.68.9.4646-4649.2002

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Infection of mice with aerosolized Mycobacterium tuberculosis: Use of a nose-only apparatus for delivery of low doses of inocula and design of an ultrasafe facility

Abstract

ASJC Scopus subject areas

UN SDGs

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