Infection of mice with aerosolized Mycobacterium tuberculosis: Use of a nose-only apparatus for delivery of low doses of inocula and design of an ultrasafe facility

J. Reid Schwebach, Bing Chen, Aharona Glatman-Freedman, Arturo Casadevall, John D. McKinney, John L. Harb, Patrick J. McGuire, W. Emmett Barkley, Barry R. Bloom, William R. Jacobs

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

Aerosolized delivery of virulent or hypervirulent Mycobacterium tuberculosis requires careful consideration of methodology and safety. To maximize safety, we installed a nose-only aerosol apparatus that can reproducibly deliver a low dose (<100 CFU per mouse) of M. tuberculosis in a carefully designed biohazard facility.

Original languageEnglish (US)
Pages (from-to)4646-4649
Number of pages4
JournalApplied and Environmental Microbiology
Volume68
Issue number9
DOIs
StatePublished - Sep 1 2002

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ASJC Scopus subject areas

  • Biotechnology
  • Food Science
  • Applied Microbiology and Biotechnology
  • Ecology

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