Induction of the granulocyte-macrophage colony-stimulating factor (CSF) receptor by granulocyte CSF increases the differentiative options of a murine hematopoietic progenitor cell

Brent L. Kreider, Paul D. Phillips, Michael B. Prystowsky, Neelam Shirsat, Jacalyn H. Pierce, Robert Tushinski, Giovanni Rovera

Research output: Contribution to journalArticle

41 Scopus citations

Abstract

32DCl3(G) is an interleukin-3-dependent murine hematopoietic precursor cell line which differentiates into neutrophilic granulocytes upon exposure to granulocyte colony-stimulating factor (G-CSF) but ceases to proliferate and dies when exposed to granulocyte-macrophage (GM)-CSF. Surface receptors for GM-CSF are undetectable on 32DCl3(G) cells but can be induced by priming the cells with G-CSF. Exposure of the G-CSF-primed cells to GM-CSF then results in the generation of monocytes as well as granulocytes. The acquired competence to respond to GM-CSF remains irreversibly encoded in the primed cells, although the GM-CSF receptor can be down regulated by interleukin-3. This phenomenon suggests a mechanism by which hematopoietic precursors may obtain additional receptors, thereby increasing their differentiative potential.

Original languageEnglish (US)
Pages (from-to)4846-4853
Number of pages8
JournalMolecular and cellular biology
Volume10
Issue number9
DOIs
StatePublished - Sep 1990
Externally publishedYes

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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