Induction of the granulocyte-macrophage colony-stimulating factor (CSF) receptor by granulocyte CSF increases the differentiative options of a murine hematopoietic progenitor cell

Brent L. Kreider, Paul D. Phillips, Michael B. Prystowsky, Neelam Shirsat, Jacalyn H. Pierce, Robert Tushinski, Giovanni Rovera

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

32DCl3(G) is an interleukin-3-dependent murine hematopoietic precursor cell line which differentiates into neutrophilic granulocytes upon exposure to granulocyte colony-stimulating factor (G-CSF) but ceases to proliferate and dies when exposed to granulocyte-macrophage (GM)-CSF. Surface receptors for GM-CSF are undetectable on 32DCl3(G) cells but can be induced by priming the cells with G-CSF. Exposure of the G-CSF-primed cells to GM-CSF then results in the generation of monocytes as well as granulocytes. The acquired competence to respond to GM-CSF remains irreversibly encoded in the primed cells, although the GM-CSF receptor can be down regulated by interleukin-3. This phenomenon suggests a mechanism by which hematopoietic precursors may obtain additional receptors, thereby increasing their differentiative potential.

Original languageEnglish (US)
Pages (from-to)4846-4853
Number of pages8
JournalMolecular and Cellular Biology
Volume10
Issue number9
StatePublished - Sep 1990
Externally publishedYes

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Granulocyte-Macrophage Colony-Stimulating Factor Receptors
Granulocyte Colony-Stimulating Factor
Granulocyte-Macrophage Colony-Stimulating Factor
Hematopoietic Stem Cells
Interleukin-3
Granulocytes
Gastrin-Secreting Cells
Mental Competency
Monocytes
Cell Line

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cell Biology

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Induction of the granulocyte-macrophage colony-stimulating factor (CSF) receptor by granulocyte CSF increases the differentiative options of a murine hematopoietic progenitor cell. / Kreider, Brent L.; Phillips, Paul D.; Prystowsky, Michael B.; Shirsat, Neelam; Pierce, Jacalyn H.; Tushinski, Robert; Rovera, Giovanni.

In: Molecular and Cellular Biology, Vol. 10, No. 9, 09.1990, p. 4846-4853.

Research output: Contribution to journalArticle

Kreider, Brent L. ; Phillips, Paul D. ; Prystowsky, Michael B. ; Shirsat, Neelam ; Pierce, Jacalyn H. ; Tushinski, Robert ; Rovera, Giovanni. / Induction of the granulocyte-macrophage colony-stimulating factor (CSF) receptor by granulocyte CSF increases the differentiative options of a murine hematopoietic progenitor cell. In: Molecular and Cellular Biology. 1990 ; Vol. 10, No. 9. pp. 4846-4853.
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abstract = "32DCl3(G) is an interleukin-3-dependent murine hematopoietic precursor cell line which differentiates into neutrophilic granulocytes upon exposure to granulocyte colony-stimulating factor (G-CSF) but ceases to proliferate and dies when exposed to granulocyte-macrophage (GM)-CSF. Surface receptors for GM-CSF are undetectable on 32DCl3(G) cells but can be induced by priming the cells with G-CSF. Exposure of the G-CSF-primed cells to GM-CSF then results in the generation of monocytes as well as granulocytes. The acquired competence to respond to GM-CSF remains irreversibly encoded in the primed cells, although the GM-CSF receptor can be down regulated by interleukin-3. This phenomenon suggests a mechanism by which hematopoietic precursors may obtain additional receptors, thereby increasing their differentiative potential.",
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