Abstract
32DCl3(G) is an interleukin-3-dependent murine hematopoietic precursor cell line which differentiates into neutrophilic granulocytes upon exposure to granulocyte colony-stimulating factor (G-CSF) but ceases to proliferate and dies when exposed to granulocyte-macrophage (GM)-CSF. Surface receptors for GM-CSF are undetectable on 32DCl3(G) cells but can be induced by priming the cells with G-CSF. Exposure of the G-CSF-primed cells to GM-CSF then results in the generation of monocytes as well as granulocytes. The acquired competence to respond to GM-CSF remains irreversibly encoded in the primed cells, although the GM-CSF receptor can be down regulated by interleukin-3. This phenomenon suggests a mechanism by which hematopoietic precursors may obtain additional receptors, thereby increasing their differentiative potential.
Original language | English (US) |
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Pages (from-to) | 4846-4853 |
Number of pages | 8 |
Journal | Molecular and cellular biology |
Volume | 10 |
Issue number | 9 |
DOIs | |
State | Published - Sep 1990 |
Externally published | Yes |
ASJC Scopus subject areas
- Molecular Biology
- Cell Biology