Induction of stringency by hyperoxia in Escherichia coli

Richard Seither, O. R. Brown

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

The effects of exposure to 4.2 atm. of oxygen on induction of the stringent genetic response (production of guanosine tetraphosphate) and the consequences for growth and survival were determined using E. coli strains NF161 stringent and NF162 relaxed. The stringent response was induced by hyperoxia, and guanosine tetraphosphate began to accumulate within 1 min in the stringent but not in the relaxed strain. Within 10 min ppGpp reached [447 ± 96] x 103 molecules/cell, approximately 6 times control values. This apparently results from the previously reported inhibition of amino acid biosynthesis by hyperoxia. During recovery with air as the gas phase, resumption of growth was correlated with the decay of ppGpp and concentrations of ppGpp and GTP were inversely correlated both during hyperoxia and recovery. Exposure to hyperoxia resulted in rapid growth cessation of both strains and to some death of the relaxed strain during 5 hr in hypoxia. After 5 hr in hyperoxia, growth of the stringent strain recovered in about 2 hr, but the relaxed strain required almost 16 hr. Thus, stringency is involved in the mechanism by which hyperoxia inhibits growth in minimal medium, and also provides some protection.

Original languageEnglish (US)
Pages (from-to)285-291
Number of pages7
JournalCellular and Molecular Biology
Volume28
Issue number3
StatePublished - 1982
Externally publishedYes

Fingerprint

Hyperoxia
Escherichia coli
Guanosine Tetraphosphate
Growth
Recovery
Biosynthesis
Guanosine Triphosphate
Transcriptional Activation
Gases
Oxygen
Amino Acids
Air
Molecules

ASJC Scopus subject areas

  • Cell Biology
  • Clinical Biochemistry
  • Molecular Biology

Cite this

Induction of stringency by hyperoxia in Escherichia coli. / Seither, Richard; Brown, O. R.

In: Cellular and Molecular Biology, Vol. 28, No. 3, 1982, p. 285-291.

Research output: Contribution to journalArticle

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