Induction of size-dependent breakdown of blood-milk barrier in lactating mice by TiO₂ nanoparticles

This study aims to investigate the potential nanotoxic effects of TiO₂ nanoparticles (TNPs) to dams and pups during lactation period. TiO₂ nanoparticles are accumulated in mammary glands of lactating mice after i.v. administration. This accumulation of TiO₂ NP likely causes a ROS-induced disruption of tight junction of the blood-milk barrier as indicated by the loss of tight junction proteins and the shedding of alveolar epithelial cells. Compared to larger TNPs (50 nm), smaller ones (8 nm) exhibit a higher accumulation in mammary glands and are more potent in causing perturbations to blood-milk barrier. An alarming finding is that the smaller TNPs (8 nm) are transferred from dams to pups through breastfeeding, likely through the disrupted blood-milk barrier. However, during the lactation period, the nutrient quality of milk from dams and the early developmental landmarks of the pups are not affected by above perturbations.