Induction of rat thyroid cell MHC class II antigen by thyrotropin and γ-interferon

M. Platzer, David S. Neufeld, L. A. Piccinini, T. F. Davies

Research output: Contribution to journalArticle

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Abstract

Major histocompatibility (MHC) class II antigen expression by thyroid epithelial cells has been widely implicated in the pathogenesis of autoimmune thyroid disease. We have examined rat MHC (RT1) class II antigen gene regulation in 1B-6 cells, cloned in our laboratory from the Fisher rat thyroid line FRT-L5. 1B-6 cells are TSH dependent for growth and proliferation, and are responsive to more than 5 μU/ml bovine TSH (bTSH) in terms of extracellular cAMP accumulation. Recombinant rat γ interferon (γIF; 1-1000 U/ml) treatment for 5 days together with bTSH (103 μU/ml) was able to induce class II antigen expression in up to 90% of 1B-6 cells, as detected by a murine monoclonal antibody to rat MHC class II antigen (FITC-OX-6). Total cellular RNA was examined in Northern blot analyses using an HLA-DR α-chain-specific RNA probe, which shares 78% sequence homology with the rat RT1.D α-chain gene. A 1.4-kilobase mRNA transcript was detected in γIF-stimulated cells, but not in untreated cells. Dose-response studies of MHC class II gene expression, using a cytoplasmic RNA slot blot technique for α-chain mRNA levels and FITC-OX 6 monoclonal antibody for detection of MHC class II antigen expression, indicated 1B-6 sensitivity to γIF in the range of 10-100 U/ml (in the presence of 103 μU/ml bTSH) and to bTSH in the range of 10-100 μU/ml (in the presence of 102 U/ml γIF). These data demonstrate dual control of MHC class II antigen gene expression by γIF and bTSH in a differentiated rat thyroid cell. Clone 1B-6 represents a powerful means of analyzing reproducibly and in long term studies the regulation of thyroid cell MHC class II gene expression.

Original languageEnglish (US)
Pages (from-to)2087-2092
Number of pages6
JournalEndocrinology
Volume121
Issue number6
StatePublished - 1987
Externally publishedYes

Fingerprint

Histocompatibility Antigens Class II
Thyrotropin
Interferons
MHC Class II Genes
Thyroid Gland
Histocompatibility
Fluorescein-5-isothiocyanate
Gene Expression
Thyrotrophs
RNA
RNA Probes
Messenger RNA
Thyroid Diseases
HLA-DR Antigens
Sequence Homology
Northern Blotting
Autoimmune Diseases
Clone Cells
Monoclonal Antibodies
Growth

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

Platzer, M., Neufeld, D. S., Piccinini, L. A., & Davies, T. F. (1987). Induction of rat thyroid cell MHC class II antigen by thyrotropin and γ-interferon. Endocrinology, 121(6), 2087-2092.

Induction of rat thyroid cell MHC class II antigen by thyrotropin and γ-interferon. / Platzer, M.; Neufeld, David S.; Piccinini, L. A.; Davies, T. F.

In: Endocrinology, Vol. 121, No. 6, 1987, p. 2087-2092.

Research output: Contribution to journalArticle

Platzer, M, Neufeld, DS, Piccinini, LA & Davies, TF 1987, 'Induction of rat thyroid cell MHC class II antigen by thyrotropin and γ-interferon', Endocrinology, vol. 121, no. 6, pp. 2087-2092.
Platzer, M. ; Neufeld, David S. ; Piccinini, L. A. ; Davies, T. F. / Induction of rat thyroid cell MHC class II antigen by thyrotropin and γ-interferon. In: Endocrinology. 1987 ; Vol. 121, No. 6. pp. 2087-2092.
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