TY - JOUR
T1 - Induction of pluripotent stem cells by defined factors is greatly improved by small-molecule compounds
AU - Huangfu, Danwei
AU - Maehr, René
AU - Guo, Wenjun
AU - Eijkelenboom, Astrid
AU - Snitow, Melinda
AU - Chen, Alice E.
AU - Melton, Douglas A.
N1 - Funding Information:
D.A.M. is a Howard Hughes Medical Institute Investigator. D.H. is funded by the Helen Hay Whitney Foundation and Novartis Institutes for BioMedical Research. W.G. is funded by the Susan G. Komen Breast Cancer Foundation. A.E.C. is supported by the Jane Coffin Childs Memorial Fund for Medical Research and Merck Research Laboratories. The authors would like to thank J. Lamenzo and A. Kweudjeu for assistance with micoarray analysis, S. Chen for insightful discussions and critical review of the manuscript. We would also like to thank R. Weinberg for support of this study. Some monoclonal antibodies were obtained from the Developmental Studies Hybridoma Bank, which was developed under the auspices of the National Institute of Child Health and Human Development and is maintained by The University of Iowa, Department of Biological Sciences.
PY - 2008/7
Y1 - 2008/7
N2 - Reprogramming of mouse and human somatic cells can be achieved by ectopic expression of transcription factors, but with low efficiencies. We report that DNA methyltransferase and histone deacetylase (HDAC) inhibitors improve reprogramming efficiency. In particular, valproic acid (VPA), an HDAC inhibitor, improves reprogramming efficiency by more than 100-fold, using Oct4-GFP as a reporter. VPA also enables efficient induction of pluripotent stem cells without introduction of the oncogene c-Myc.
AB - Reprogramming of mouse and human somatic cells can be achieved by ectopic expression of transcription factors, but with low efficiencies. We report that DNA methyltransferase and histone deacetylase (HDAC) inhibitors improve reprogramming efficiency. In particular, valproic acid (VPA), an HDAC inhibitor, improves reprogramming efficiency by more than 100-fold, using Oct4-GFP as a reporter. VPA also enables efficient induction of pluripotent stem cells without introduction of the oncogene c-Myc.
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U2 - 10.1038/nbt1418
DO - 10.1038/nbt1418
M3 - Article
C2 - 18568017
AN - SCOPUS:46949085597
SN - 1087-0156
VL - 26
SP - 795
EP - 797
JO - Nature biotechnology
JF - Nature biotechnology
IS - 7
ER -