Induction of pluripotent stem cells by defined factors is greatly improved by small-molecule compounds

Danwei Huangfu, René Maehr, Wenjun Guo, Astrid Eijkelenboom, Melinda Snitow, Alice E. Chen, Douglas A. Melton

Research output: Contribution to journalArticle

Abstract

Reprogramming of mouse and human somatic cells can be achieved by ectopic expression of transcription factors, but with low efficiencies. We report that DNA methyltransferase and histone deacetylase (HDAC) inhibitors improve reprogramming efficiency. In particular, valproic acid (VPA), an HDAC inhibitor, improves reprogramming efficiency by more than 100-fold, using Oct4-GFP as a reporter. VPA also enables efficient induction of pluripotent stem cells without introduction of the oncogene c-Myc.

LanguageEnglish (US)
Pages795-797
Number of pages3
JournalNature Biotechnology
Volume26
Issue number7
DOIs
StatePublished - Jul 2008
Externally publishedYes

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Pluripotent Stem Cells
Stem Cell Factor
Histone Deacetylase Inhibitors
Valproic Acid
Stem cells
Molecules
Methyltransferases
Oncogenes
Transcription factors
Transcription Factors
Acids
DNA
Histone Deacetylases
Ectopic Gene Expression

ASJC Scopus subject areas

  • Microbiology

Cite this

Huangfu, D., Maehr, R., Guo, W., Eijkelenboom, A., Snitow, M., Chen, A. E., & Melton, D. A. (2008). Induction of pluripotent stem cells by defined factors is greatly improved by small-molecule compounds. Nature Biotechnology, 26(7), 795-797. https://doi.org/10.1038/nbt1418

Induction of pluripotent stem cells by defined factors is greatly improved by small-molecule compounds. / Huangfu, Danwei; Maehr, René; Guo, Wenjun; Eijkelenboom, Astrid; Snitow, Melinda; Chen, Alice E.; Melton, Douglas A.

In: Nature Biotechnology, Vol. 26, No. 7, 07.2008, p. 795-797.

Research output: Contribution to journalArticle

Huangfu, D, Maehr, R, Guo, W, Eijkelenboom, A, Snitow, M, Chen, AE & Melton, DA 2008, 'Induction of pluripotent stem cells by defined factors is greatly improved by small-molecule compounds' Nature Biotechnology, vol. 26, no. 7, pp. 795-797. https://doi.org/10.1038/nbt1418
Huangfu, Danwei ; Maehr, René ; Guo, Wenjun ; Eijkelenboom, Astrid ; Snitow, Melinda ; Chen, Alice E. ; Melton, Douglas A. / Induction of pluripotent stem cells by defined factors is greatly improved by small-molecule compounds. In: Nature Biotechnology. 2008 ; Vol. 26, No. 7. pp. 795-797.
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